Lacrimal glands, regional draining cervical lymph nodes (CLN) were harvested from the experimental murine groups at the end of the study, and single cell suspensions were prepared. In order to increase total cell counts multiple tissue samples were combined. Cells were stained with the following fluorescent conjugated antibodies: anti-CD4 eFluor450 (RM4–5), anti-CD25 APC-Cy7 (PC61), anti-FoxP3 PE (FJK-16s) (eBioscience, San Diego, CA and BD Bioscience, San Jose, CA). For intracellular cytokine staining, the cells were placed in a 96-well plate overnight in cell culture media with Cell Stimulation Cocktail (plus protein transport inhibitor, eBioscience) and stained with anti-IFN-γ FITC (XMG 1.2) (eBioscience, San Diego, CA). Absolute counting beads were used for the lacrimal gland (ThermoFisher Scientific, Waltham, MA). Stained cells were analyzed using FlowJO (Ashland, OR).
Anti ifn γ fitc xmg 1.2
Manufactured by Thermo Fisher Scientific
Anti-IFN-γ FITC (XMG 1.2) is a fluorescently-labeled antibody that binds to interferon-gamma (IFN-γ). This antibody can be used for the detection and measurement of IFN-γ in various experimental applications.
Automatically generated - may contain errors
Lab products found in correlation
2 protocols using anti ifn γ fitc xmg 1.2
1
Immune Cell Analysis in Murine Lacrimal Glands
2
Comprehensive Multiparameter Immune Cell Profiling
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
For analysis of surface markers, cells were stained in PBS containing 2% (wt/vol) BSA, with anti-CD4-PB (RM4-5) (Biolegend, 100531, 1:200), anti-CD8α-BV786 (53-6.7) (BD Horizon, 563332, 1:200), anti-TCRβ-APC Cy7 (H57-597) (BD Pharmingen, 560656, 1:200), anti-CD45.2-BV605 (104) (Biolegend, 109841, 1:200), anti-PD-1-APC (RMP1-30) (eBiosciences, 17-9981-82, 1:200), anti-PD-L1-BV711 (10F.9G2) (Biolegend, 124319, 1:100) and anti-CTLA-4-PE (UC10-4B9) (eBiosciences, 12-1522-82, 1:200) on ice for 30 min. Intracellular anti-Foxp3-AF532 (FJK-16 s) (eBiosciences, 58-5773-82, 1:100), anti-IL-2-PerCp Cy5.5 (PC61.5) (eBiosciences, 45-0251-82, 1:100), anti-Ki-67-PE-Cy7 (SolA15) (eBiosciences, 25-5698-82, 1:300), anti-IFN-γ-FITC (XMG1.2) (eBioscience, 11-7311-82, 1:200) and anti-CTLA-4-PE (UC10-4B9) (eBiosciences, 12-1522-82, 1:200), anti-TNF-α-BV650 (MP6-XT22) (Biolegend, 506333, 1:100) were analyzed by flow cytometry according to the manufacturer's instructions. Flow cytometry data were acquired on LSRII or BD FACS Canton II (BD Biosciences) and analyzed using Flowjo software (Tree Star).
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!