The following primary antibodies were used: anti-GFP (Rabbit, 1:250, 2956, Cell Signaling), anti-GFP (mouse, 1:250, 2955, Cell Signaling), anti-GFP (Chicken, 1:2000, ab13970, Abcam), anti-K5 (rabbit, 1:2400, Biolegend), anti-K8 (mouse, 1:2000, MMS-162P, Biolegend), anti-p63 (rabbit, 1:2000, Biolegend), anti-PCNA (mouse, 1:200, sc-15, Santa Cruz), anti-E-cadherin (mouse, 1:300, c20820, BD Transduction Laboratories), anti-androgen receptor (rabbit, 1:500, PA-15780, Thermo Fisher), Biotinylated anti-rabbit or anti-mouse secondary antibodies (BA-1000 or BA-9200, Vector Laboratories) were used for immunohistochemistry experiments. For immunofluorescence studies, AlexaFluor-conjugated anti-rabbit, anti-mouse or anti-chicken antibodies were used (Cell Signaling).
Mms 162p
Manufactured by BioLegend
The MMS-162P is a high-performance microplate sealer designed for reliable and secure sealing of microplates. It features an automatic sealing process and can accommodate various sealing materials such as films, foils, and tapes. The device is suitable for use in a wide range of laboratory applications.
Automatically generated - may contain errors
Lab products found in correlation
Lsm 510 meta confocal microscope, by Zeiss (2 mentions)
C20820, by BD (1 mentions)
Sc 15, by Santa Cruz Biotechnology (1 mentions)
Ba 1000, by Vector Laboratories (1 mentions)
Ba 9200, by Vector Laboratories (1 mentions)
Ab13970, by Abcam (1 mentions)
Prb 160p, by BioLegend (1 mentions)
Axioplan 2, by Zeiss (1 mentions)
Alexa fluor 594 phalloidin, by Thermo Fisher Scientific (1 mentions)
Prb 155p, by BioLegend (1 mentions)
2 protocols using mms 162p
1
Comprehensive Immunohistochemical Profiling
2
Immunofluorescence Analysis of Epithelial Cell Markers
Check if the same lab product or an alternative is used in the 5 most similar protocols
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Tissues sections were obtained and analysed as previously described [27 (link)]. The following antibodies were used: cytokeratin 5 (1:500, PRB-160P; BioLegend, San Diego, CA), cytokeratin 14 (1:500, PRB-155P; BioLegend), cytokeratin 8 (1:500, MMS-162P; Biolegend), p63 (1:100, sc-8431, clone 4A4; Santa Cruz Biotechnologies, Santa Cruz, CA), integrin β1 (10 mg/ml, MAB1997, clone MB1.2, Chemicon, Temecula, CA), Alexa Fluor 594 phalloidin (1:100, Molecular Probes, Eugene, OR), alpha smooth muscle actin–cy3 (1:400, C6198, Sigma), Wif1 (1:100, ab33281, abcam, Paris, France). Secondary antibodies were from Molecular Probe: Alexa Fluor 594 F(ab’)2 fragment of goat anti-rabbit IgG (1:1000, A11072), Alexa Fluor 594 F(ab’)2 fragment of goat anti-mouse IgG (1:1000, A11020), Alexa Fluor 594 F(ab’)2 fragment of goat anti-Rat IgG (1:1000, A11007). Pictures were taken either on a LSM 510 META Confocal Microscope or an Axioplan2 with ApoTome equipment (Carl Zeiss, Germany).
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