Phenol red free rpmi 1640 medium

Manufactured by Merck Group

Sourced in United States

Phenol Red-free RPMI-1640 medium is a cell culture medium that does not contain the pH indicator phenol red. It is formulated to support the growth and maintenance of a variety of cell types in vitro.

Automatically generated - may contain errors

Lab products found in correlation

Propidium iodide, by Merck Group (1 mentions) Calceinacetoxymethyl ester calcein am, by Dojindo Laboratories (1 mentions) Recombinant mouse il 2, by Roche (1 mentions) Rpmi 1640 medium, by Merck Group (1 mentions) Rpmi 1640 medium, by Thermo Fisher Scientific (1 mentions) 17β estradiol e2, by Merck Group (1 mentions) Charcoal dextran stripped fbs, by Thermo Fisher Scientific (1 mentions) Fetal bovine serum (fbs), by Harvard Bioscience (1 mentions)

Close spelling variants of this product

RPMI-1640 medium (3704 citations) RPMI 1640 (3389 citations) Phenol red (423 citations) Phenol (410 citations) RPMI medium (384 citations) 1640 medium (114 citations) Phenol red-free RPMI 1640 (4 citations) Phenol red free RPMI (3 citations)

2 protocols using phenol red free rpmi 1640 medium

Murine NK Cell Isolation and Activation

Check if the same lab product or an alternative is used in the 5 most similar protocols

RPMI-1640 medium, Phenol Red-free RPMI-1640 medium, propidium iodide, and
3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2H-tetrazolium
bromide (MTT) were obtained from Sigma-Aldrich, Inc (St Louis, MO). Calcein
acetoxymethyl ester (calcein-AM) was purchased from Dojindo Laboratories
(Kumamoto, Japan). Recombinant mouse IL-2 was obtained from Roche Applied
Science (Indianapolis, IN). Oligodeoxynucleotide (ODN) 1585 and its negative
control were purchased from InvivoGen (San Diego, CA). Mouse NK cell separation
set-DM, mouse NK cell enrichment set-DM, and phycoerythrin (PE)-conjugated rat
anti-mouse NK1.1, fluorescein isothiocyanate (FITC)-conjugated hamster
anti-mouse CD3 ϵ chain, and rat anti-mouse CD16/CD32 monoclonal antibodies
(mAbs) were obtained from BD Biosciences (San Jose, CA).

Sakaguchi K., Zhong M., Kawai S., Shimizu Y, & Gohda E. (2016). Augmentation of Cytolytic Activity in Murine Natural Killer Cells and Inhibition of Tumor Growth by the Ethanol Fraction of Oyster Extract. Integrative Cancer Therapies, 17(1), 31-40.

+ Open protocol

+ Expand

Culturing SK-N-SH cells for BPA/E2 treatment

Check if the same lab product or an alternative is used in the 5 most similar protocols

SK-N-SH human NB cells (obtained from Shanghai Institute for Biological Sciences, Chinese Academy of Sciences) and SK-N-SH subtype cells sorted by FCM were cultured in RPMI 1640 medium (Gibco, Scotland, UK) containing 10% fetal bovine serum (FBS; Biochrom, Berlin, Germany), 100 U/mL penicillin, and 100 mg/mL streptomycin. Cells were incubated at 37°C in a humidified atmosphere with 5% CO 2 . Before treatment with 20 μg/mL BPA (Shanghai Chemical Reagents Company, Shanghai, China) or 1 ng/mL 17β-estradiol (E 2 ; Sigma, St. Louis, MO, USA), the cells were seeded in phenol red-free RPMI 1640 medium (Sigma) supplemented with 10% charcoal-dextran-stripped FBS (Gibco) for 48 h to exhaust the endogenous estrogen.

Zheng J.C., Qi S.Q., Yang L., Ma Y.Y., Dong K.R., Zhu H.T., Yang S.B., Xu T., Zheng S, & Xiao X.M. (2015). Effects of bisphenol A on decreasing the percentage and promoting the growth of stem cell-like cells from SK-N-SH human neuroblastoma cells. Genetics and molecular research : GMR, 14(2).

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!