Ah6809

Manufactured by Bio-Techne

Sourced in United Kingdom, United States

The AH6809 is a laboratory equipment product developed by Bio-Techne. It is a device used for scientific research and analysis purposes. No further details about its core function or intended use can be provided in an unbiased and factual manner.

Automatically generated - may contain errors

Lab products found in correlation

Forskolin, by Bio-Techne (2 mentions) 16 16 dimethyl pge2, by Bio-Techne (2 mentions) Celecoxib, by Merck Group (2 mentions) Trizol, by Thermo Fisher Scientific (2 mentions) L161982, by Bio-Techne (1 mentions) Ly29004, by Cell Signaling Technology (1 mentions) Niflumic acid, by Cayman Chemical (1 mentions) Xav939, by Bio-Techne (1 mentions) Bromodeoxyuridine (brdu), by Merck Group (1 mentions) L 161 982, by Merck Group (1 mentions) Mouse anti brdu antibody, by Merck Group (1 mentions) Mustard oil, by Merck Group (1 mentions) Ionomycin, by Thermo Fisher Scientific (1 mentions) Pf 04418948, by Bio-Techne (1 mentions) Ox papc, by Hycult Biotech (1 mentions) Complete freund s adjuvant cfa, by Rockland Immunochemicals (1 mentions) Capsaicin, by Merck Group (1 mentions) Trypan blue, by Bio-Rad (1 mentions) Roswell park memorial institute (rpmi), by Thermo Fisher Scientific (1 mentions) Tc20 automated cell counter, by Bio-Rad (1 mentions)

6 protocols using ah6809

Pharmacological Inhibitors for Epac1 and Epac2

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16,16-dimethyl-PGE₂, L161,982, AH6809 and Forskolin were from Tocris Bioscience (Bristol, UK). Ly29004 was from Cell Signaling (Beverly, MA). The pharmacological inhibitor for Epac1, CE3F4 was developed by F. Lezoualc'h [37 (link)]. The pharmacological inhibitor for Epac2, ESI-05 was developed by X. Cheng [38 (link)]. TRIzol® was from Thermo Fisher Scientific (Waltham, MA). All other chemicals were of analytical grade.

Jansen S.R., Poppinga W.J., de Jager W., Lezoualc'h F., Cheng X., Wieland T., Yarwood S.J., Gosens R, & Schmidt M. (2016). Epac1 links prostaglandin E2 to β-catenin-dependent transcription during epithelial-to-mesenchymal transition. Oncotarget, 7(29), 46354-46370.

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Analyzing Cell Proliferation Signaling

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BrdU, mouse anti-BrdU antibody, PI, L-161,982 and celecoxib were purchased from Sigma-Aldrich. 16,16-dimethyl-PGE₂, XAV939, AH6809 and forskolin were from Tocris Bioscience (Bristol, UK). Niflumic acid was from Cayman Chemical (Ann Arbor, MI, USA). All other chemicals were of analytical grade.

Jansen S.R., Holman R., Hedemann I., Frankes E., Elzinga C.R., Timens W., Gosens R., de Bont E.S, & Schmidt M. (2014). Prostaglandin E2 promotes MYCN non-amplified neuroblastoma cell survival via β-catenin stabilization. Journal of Cellular and Molecular Medicine, 19(1), 210-226.

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Nociceptive Response Evaluation Protocol

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OxPAPC, PAPC and DMPC were purchased from Hycult Biotech (Plymouth Meeting, PA, USA); Complete Freund’s adjuvant (CFA) was purchased from Rockland Immunochemicals (Gilbertsville, PA, USA); Ionomycin was from Invitrogen (Grand Island, NY, USA). AH6809 and PF04418948 were obtained from Tocris (Minneapolis, MN, USA). Capsaicin, mustard oil and other reagents were obtained from Sigma-Aldrich (St. Louis, MO, USA)

Liu B., Tai Y., Caceres A.I., Achanta S., Balakrishna S., Shao X., Fang J, & Jordt S.E. (2016). Oxidized Phospholipid OxPAPC Activates TRPA1 and Contributes to Chronic Inflammatory Pain in Mice. PLoS ONE, 11(11), e0165200.

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Modulation of Pre-B-Cell Line Response to PGE2

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Human pre-B-cell lines 697 (ACC42 DSMZ collection) and SUP-B15 (ACC389 DSMZ collection) were cultivated in RPMI (Gibco) supplemented with 10% FBS. 5x10⁵ cells per ml were pre-incubated with either the EP1/EP2 receptor antagonist AH6809 (10 μM, Tocris) or the EP4 receptor antagonist GW627368 (10 μM, Tocris) for 2 h. PGE2 (10 μM, Sigma-Aldrich) was added and cells were harvested after 48 h in TRIzol, or stained with trypan blue (Bio-Rad laboratories) and counted for measuring live to dead ratio and cell numbers using the TC20 automated cell counter (Bio-Rad laboratories). Control cells were incubated with dissolvents (DMSO or ethanol (ETHO), 1 μL/ml media). Alternatively, 5x10⁵ per ml 697 and SUP-B15 cells were incubated with 10% human serum from older individuals (>60 y) prior to the commencement of the exercise program at baseline (BL) and after 12M (12M FU) for 48 h and harvested in TRIzol. SUP-B15 cells were incubated with 10% human serum from COVID-19 patients and from healthy controls. Cells were harvested after 48 h in TRIzol.

Ricke-Hoch M., Stelling E., Lasswitz L., Gunesch A.P., Kasten M., Zapatero-Belinchón F.J., Brogden G., Gerold G., Pietschmann T., Montiel V., Balligand J.L., Facciotti F., Hirsch E., Gausepohl T., Elbahesh H., Rimmelzwaan G.F., Höfer A., Kühnel M.P., Jonigk D., Eigendorf J., Tegtbur U., Mink L., Scherr M., Illig T., Schambach A., Pfeffer T.J., Hilfiker A., Haverich A, & Hilfiker-Kleiner D. (2021). Impaired immune response mediated by prostaglandin E2 promotes severe COVID-19 disease. PLoS ONE, 16(8), e0255335.

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Monocyte Modulation by Tumor-Derived Factors

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Human monocytes were isolated from peripheral blood as described previously (9) and cultured in RPMI1640 containing 10% FBS, 2 mmol/L glutamine, 100 U/mL penicillin-streptomycin. Culture of human pancreatic carcinoma cell line PANC1 and preparation of tumor-conditioned supernatant (TSN) are described in the Supplementary Materials and Methods.
The concentration for the different treatments were as follows: PANC1 supernatant (30%), human IFNg (PeproTech) 20 ng/mL, prostaglandin E2 (PGE2) receptor (EP2/EP1) antagonist AH6809 (Tocris) 10 À5 mol/L.

Porta C., Consonni F.M., Morlacchi S., Sangaletti S., Bleve A., Totaro M.G., Larghi P., Rimoldi M., Tripodo C., Strauss L., Banfi S., Storto M., Pressiani T., Rimassa L., Tartari S., Ippolito A., Doni A., Soldà G., Duga S., Piccolo V., Ostuni R., Natoli G., Bronte V., Balzac F., Turco E., Hirsch E., Colombo M.P, & Sica A. (2020). Tumor-Derived Prostaglandin E2 Promotes p50 NF-κB-Dependent Differentiation of Monocytic MDSCs. Cancer research, 80(13).

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Mechanistic Study of Targeted Cancer Therapies

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Arsenic trioxide (AS₂O₃) and COX2 selective inhibitor celecoxib were purchased from Sigma-Aldrich (St. Louis, USA). Prostaglandin E2 (PGE2) was purchased from Cayman Chemical (Ann Arbor, USA). EGFR small molecule inhibitor erlotinib was purchased from BioVision (Milpitas, USA). PGE2 receptor 4 (EP4) antagonist ONO-AE3-208, EP3 antagonist L-798,106, EP1 and EP2 antagonist AH 6809, and COX2 selective inhibitor etodolac were purchased from Tocris Bioscience (Ellisville, USA).

Ooki A., Begum A., Marchionni L., VandenBussche C.J., Mao S., Kates M, & Hoque M.O. (2018). Arsenic Promotes the COX2/PGE2-SOX2 Axis to Increase the Malignant Stemness Properties of Urothelial Cells. International journal of cancer, 143(1), 113-126.

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