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Anti chicken

Manufactured by Jackson ImmunoResearch
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Anti-chicken is a laboratory reagent produced by Jackson ImmunoResearch. It is designed for use in various immunological and biochemical applications that require the detection or identification of chicken-derived proteins or other analytes.

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Lab products found in correlation

Anti mouse, by Jackson ImmunoResearch (3 mentions) Anti rabbit, by Jackson ImmunoResearch (3 mentions) Ab13822, by BD (2 mentions) Alexa fluor 488, by Thermo Fisher Scientific (2 mentions) Hpa018425, by Merck Group (2 mentions) F3648, by Merck Group (2 mentions) Mab1378, by Merck Group (2 mentions) Nki goh3, by Merck Group (2 mentions) Twist2c1a, by Merck Group (2 mentions) T6451, by Merck Group (2 mentions) H 125, by Santa Cruz Biotechnology (2 mentions) Ab50887, by Merck Group (2 mentions) Anti e cadherin, by Abcam (2 mentions) Anti e cadherin, by BD (2 mentions) Mouse anti actin, by Merck Group (1 mentions) Rabbit anti prx2, by Abcam (1 mentions) Rabbit anti prx1, by Cell Signaling Technology (1 mentions) Rabbit anti myc, by Cell Signaling Technology (1 mentions) Irdye 680rd goat anti mouse, by LI COR (1 mentions) Mouse anti gfp, by Merck Group (1 mentions)

3 protocols using anti chicken

1

Antibody Panel for EMT Markers

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Primary antibodies include anti-β-actin (Abcam, ab13822, 1:3000), anti-E-cadherin (BD, 610182, 1:200 for immunostaining, 1:1000 for Western blotting), anti-E-cadherin (Abcam, ab11512, Decma-1, 1:200), anti-G3BP2 (Sigma-Aldrich , HPA018425, 1:200, 1:1000), anti-Fibronectin (Sigma-Aldrich, F3648, 1:200), anti-Integrin α6 (Millipore, MAB1378, NKI-GoH3, 1:200), anti-human Laminin V (Chemicon, D4B5, 1:200), anti-mouse Laminin V (kind gift from M. Aumailley, 1:1000), anti-Twist1 (Santa Cruz, ab50887, Twist2C1a, 1:100, 1:1000), Rabbit anti-Twist1 (Sigma-Aldrich, T6451, 1:1000), 5b7 mouse anti-Twist1 hybridoma cell line (1:1000), anti-YAP1 (Santa Cruz, H-125, 1:100). AIIB2 hybridoma supernatant was used for β1 integrin blocking experiments (Developmental Studies Hybridoma Bank, 1:1000). Secondary fluorescent antibodies used include anti-mouse, anti-rat, and anti-rabbit conjugated with Alexafluor 488, 546, and 647 (Life Technologies). Secondary horseradish peroxidase (HRP) conjugated antibodies used include anti-mouse, anti-rabbit, and anti-chicken (Jackson Immunoresearch).
Wei S.C., Fattet L., Tsai J.H., Guo Y., Pai V.H., Majeski H.E., Chen A.C., Sah R.L., Taylor S.S., Engler A.J, & Yang J. (2015). Matrix stiffness drives Epithelial-Mesenchymal Transition and tumour metastasis through a TWIST1-G3BP2 mechanotransduction pathway. Nature cell biology, 17(5), 678-688.
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2

Antibody Validation and Immunoblotting Protocol

Check if the same lab product or an alternative is used in the 5 most similar protocols
Antibodies used were chicken anti-APEX2 (Innovagen, 126-146), mouse anti-ß-Actin (Sigma, A5441), mouse anti-flag (Sigma, F3165), mouse anti-GFP (Sigma, G1546), rabbit anti-Prx1 (Cell Signaling, #8499), rabbit anti-Prx2 (Abcam, 109367) and rabbit anti-myc (Cell Signaling, 2278). All primary antibodies were used at a dilution of 1:1000, except for mouse anti-flag which was used at a dilution of 1:30,000. The HRP-conjugated secondary antibodies used for immunoblotting were anti-chicken (Jackson ImmunoResearch, 703-035-155), anti-mouse (Jackson ImmunoResearch, 115-035-146) and anti-rabbit (Jackson ImmunoResearch, 111-035-144), all used at a dilution of 1:10,000. The fluorescent secondary antibodies used were IRDye 800CW donkey anti-chicken (Li-Cor, 926-32218), used at 1:5000, and IRDye 680RD goat anti-mouse (Li-Cor, 926-68070), used at a dilution of 1:10,000.
Eid M., Barayeu U., Sulková K., Aranda-Vallejo C, & Dick T.P. (2024). Using the heme peroxidase APEX2 to probe intracellular H2O2 flux and diffusion. Nature Communications, 15, 1239.
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3

Antibody Panel for EMT Markers

Check if the same lab product or an alternative is used in the 5 most similar protocols
Primary antibodies include anti-β-actin (Abcam, ab13822, 1:3000), anti-E-cadherin (BD, 610182, 1:200 for immunostaining, 1:1000 for Western blotting), anti-E-cadherin (Abcam, ab11512, Decma-1, 1:200), anti-G3BP2 (Sigma-Aldrich , HPA018425, 1:200, 1:1000), anti-Fibronectin (Sigma-Aldrich, F3648, 1:200), anti-Integrin α6 (Millipore, MAB1378, NKI-GoH3, 1:200), anti-human Laminin V (Chemicon, D4B5, 1:200), anti-mouse Laminin V (kind gift from M. Aumailley, 1:1000), anti-Twist1 (Santa Cruz, ab50887, Twist2C1a, 1:100, 1:1000), Rabbit anti-Twist1 (Sigma-Aldrich, T6451, 1:1000), 5b7 mouse anti-Twist1 hybridoma cell line (1:1000), anti-YAP1 (Santa Cruz, H-125, 1:100). AIIB2 hybridoma supernatant was used for β1 integrin blocking experiments (Developmental Studies Hybridoma Bank, 1:1000). Secondary fluorescent antibodies used include anti-mouse, anti-rat, and anti-rabbit conjugated with Alexafluor 488, 546, and 647 (Life Technologies). Secondary horseradish peroxidase (HRP) conjugated antibodies used include anti-mouse, anti-rabbit, and anti-chicken (Jackson Immunoresearch).
Wei S.C., Fattet L., Tsai J.H., Guo Y., Pai V.H., Majeski H.E., Chen A.C., Sah R.L., Taylor S.S., Engler A.J, & Yang J. (2015). Matrix stiffness drives Epithelial-Mesenchymal Transition and tumour metastasis through a TWIST1-G3BP2 mechanotransduction pathway. Nature cell biology, 17(5), 678-688.
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