Zetasizer nano series

DLS (Malvern-Zetasizer Nano ZS90)
was used to determine the average hydrodynamic diameter, Z-potential, and PDI of L-PDNPs; 100 μg/mL of L-PDNPs in Milli-Q
water was used for all the measurements using disposable polystyrene
cuvettes (Malvern Zetasizer Nano series) to measure the hydrodynamic
diameter and disposable folded capillary cells (Malvern Zetasizer
Nano series) for measuring surface Z-potential.

Conjugation of TEMPO and PEG₂₀₀₀-NH₂.HCl to the carboxylate group on the surface of the PY-LCNPs was confirmed by agarose (1%) gel electrophoresis under an applied voltage of 95 V for 30 min at room temperature in 1× tris-buffered saline (TBS). Particle size distribution and sample concentration were measured by dynamic light scattering (DLS) of LCNP solution (40× dilution of the original suspension) in water using a ZetaSizer NanoSeries equipped with a HeNe laser source (λ = 633 nm) (Malvern Instruments Ltd., Worcestershire, UK) and analyzed using Dispersion Technology Software (DTS, Malvern Instruments Ltd., Worcestershire, UK). Zeta-potential was measured on a ZetaSizer NanoSeries equipped with a HeNe laser source (λ = 633 nm) (Malvern Instruments Ltd., Worcestershire, UK) and an avalanche photodiode for detection. For each analysis, at least four measurements were performed, and the data were reported as average values ± standard error of the mean (SEM).

Dynamic light scattering (DLS) measurements were carried out using ZetaSizer NanoSeries equipped with a HeNe laser source (λ = 633 nm) (Malvern Instruments Ltd, Worcestershire, UK) and analyzed using Dispersion Technology Software (DTS, Malvern Instruments Ltd, Worcestershire, UK). MVs were loaded into disposable cells, and data were collected at 25 °C. All the samples were prepared in 0.1 x PBS buffer pH 7.4. For each sample, the autocorrelation function was the average of five runs of 10 seconds each and then repeated about three to six times. CONTIN analysis was then used to number versus hydrodynamic size profiles for the dispersions studied.
For Zeta-Potential (ζ-potential) measurement, Laser Doppler Velocimetry (LDV) measurements were performed using a ZetaSizer NanoSeries equipped with a HeNe laser source (λ = 633 nm) (Malvern Instruments Ltd, Worcestershire, UK) and an avalanche photodiode for detection, controlled with DTS software. MVs were loaded into disposable cells, and data were collected at 25 °C. Three runs of the measurements were performed for each sample to achieve the zeta potential. All the samples were prepared in 0.1 × PBS buffer pH 7.4. For both DLS and zeta-potential, MVs were used at a 10-fold dilution from stock concentration.