Log In Sign up
The largest database of trusted experimental protocols

Eclipse e800

Manufactured by Hamamatsu Photonics
Visit Supplier

The Eclipse E800 is a research-grade microscope system designed for a variety of applications. It features a modular design, allowing for the integration of various components and accessories to meet the specific needs of the user. The core function of the Eclipse E800 is to provide a stable and reliable platform for high-resolution imaging and analysis.

Automatically generated - may contain errors

Lab products found in correlation

Cytokeratin 7 ck7, by Abcam (1 mentions) Ndp view2, by Hamamatsu Photonics (1 mentions) Nanozoomer xr, by Hamamatsu Photonics (1 mentions) Lsm700 confocal microscope, by Adobe (1 mentions) Orca er, by Hamamatsu Photonics (1 mentions) Nis element, by Nikon (1 mentions) Orca er, by PerkinElmer (1 mentions)

4 protocols using eclipse e800

1

Histological Analysis of Recellularized Liver Grafts

Check if the same lab product or an alternative is used in the 5 most similar protocols
The native liver tissue and recellularized liver grafts were fixed with 10% formalin for 24 hours, embedded in paraffin, and processed at the Specialized Histopathology Services Core at MGH for staining with hematoxylin and eosin (H&E), 1:100 diluted cytokeratin-7 (CK7) (Abcam, Cambridge, UK) with 1:250 diluted biotinylated goat secondary anti-rabbit (Vector Laboratories, Inc., Burlingame, CA) and 1:25 diluted Ki-67 (Leica Biosystems, Wetzlar, Germany) with mouse HRP (DAKO Agilent, Santa Clara, CA). The dilutions of antibodies were selected following manufacturer’s recommendations for application in immunohistochemistry. The slides were imaged using Nikon Eclipse E800 and Hamamatsu NanoZoomer-XR, and subsequently processed with NDP.view 2 from Hamamatsu Photonics.
Chen Y., Devalliere J., Bulutoglu B., Yarmush M.L, & Uygun B.E. (2019). Repopulation of intrahepatic bile ducts in engineered rat liver grafts. Technology, 7(1-2), 46-55.
+ Open protocol
+ Expand
2

Fluorescence Microscopy Imaging Protocol

Check if the same lab product or an alternative is used in the 5 most similar protocols
Images were acquired on a Nikon Eclipse E800 with Hamamatsu Orca-ER digital camera using Openlab software and processed in Adobe Photoshop CS5. Images showing staining for Hck were acquired on an LSM-700 Confocal microscope using Zen software and processed in Adobe Photoshop CS5. For consistency, images depicting total bacteria, including bacteria labeled with CFSE, were false colored blue.
Johnson M.B., Ball L.M., Daily K.P., Martin J.N., Columbus L, & Criss A.K. (2014). Opa+ Neisseria gonorrhoeae Exhibits Reduced Survival in Human Neutrophils Via Src Family Kinase-Mediated Bacterial Trafficking Into Mature Phagolysosomes. Cellular microbiology, 17(5), 648-665.
+ Open protocol
+ Expand
3

Fluorescence Microscopy Image Acquisition

Check if the same lab product or an alternative is used in the 5 most similar protocols
Slides were imaged on a Nikon Eclipse E800 UV/visible fluorescence microscope with Hamamatsu Orca-ER digital camera using Openlab (Improvision) or NIS Elements (Nikon) software. Images were exported and processed using Adobe Photoshop CC2019.
Smirnov A., Daily K.P., Gray M.C., Ragland S.A., Werner L.M., Brittany Johnson M., Eby J.C., Hewlett E.L., Taylor R.P, & Criss A.K. (2023). Phagocytosis via complement receptor 3 enables microbes to evade killing by neutrophils. Journal of leukocyte biology, 114(1).
+ Open protocol
+ Expand
4

DAPI Staining for Microscopy

Check if the same lab product or an alternative is used in the 5 most similar protocols
Approximately 0.1 OD600 of cells was stained with 4′,6-diamidino-2-phenylindole (DAPI, final concentration 0.1 μg/mL) following a 2 min incubation in 70% ethanol at room temperature. Images were obtained with the epifluorescence Nikon Eclipse E800 microscope (100× objective) using the Hamamatsu digital CCD camera.
Skowronek E., Grzechnik P., Späth B., Marchfelder A, & Kufel J. (2014). tRNA 3′ processing in yeast involves tRNase Z, Rex1, and Rrp6. RNA, 20(1), 115-130.
+ Open protocol
+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required

Sign up now

Revolutionizing how scientists
search and build protocols!