Oxaliplatin

Manufactured by Targetmol

Sourced in China, United States

Oxaliplatin is a platinum-based chemotherapeutic agent used for the treatment of various types of cancer. It functions as a DNA-damaging agent, interfering with the replication and transcription of genetic material, leading to cell death.

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Lab products found in correlation

Glutamax, by Thermo Fisher Scientific (2 mentions) Crystal violet, by Solarbio (1 mentions) Sodium pyruvate, by Thermo Fisher Scientific (1 mentions) β cateninmut plasmid, by Addgene (1 mentions) Penicillin g and streptomycin, by Thermo Fisher Scientific (1 mentions) Dulbecco s modified eagle medium dmem, by Thermo Fisher Scientific (1 mentions) Puromycin, by Solarbio (1 mentions) Doxorubicin, by Targetmol (1 mentions) Docetaxel, by Targetmol (1 mentions) Gabapentin, by Targetmol (1 mentions) Ionomycin, by Cayman Chemical (1 mentions) Anti cd8 antibody, by Miltenyi Biotec (1 mentions) Ficoll, by GE Healthcare (1 mentions) Ultra low attachment microplate 7007, by Corning (1 mentions)

6 protocols using oxaliplatin

Comprehensive Chemicals for Cell Culture

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Fludarabine phosphate (T6501), oxaliplatin (T0164), docetaxel (T1034), capecitabine (T1408), doxorubicin (T1456), irinotecan hydrochloride (T0486L), carboplatin (T1058), and calcium folinate (T0148) were purchased from TargetMol Chemicals (Shanghai, China). 5-Fluorouracil (5-FU, S1209), sorafenib (S7397), gemcitabine (S1149), cisplatin (S1166), and MTX (S5097) were purchased from Selleck Biotechnology (Shanghai, China). Dulbecco's Modified Eagle Medium (DMEM, #11995-065), Roswell Park Memorial Institute (RPMI) 1640 (#72400047), fetal bovine serum (#10100147C), non-essential amino acid solution (#11140050), penicillin G and streptomycin (#15140163), glutamax (#35050061), and sodium pyruvate (#11360070) were purchased from Thermo Fisher (Carlsbad, CA, USA). Horse serum was purchased from Procell (#164215, Wuhan, Hubei, China). Puromycin (#P8230), polybrene (#H8761), and 0.1% crystal violet (#G1063) were purchased from Solarbio (Beijing, China). The β-catenin^mut plasmid was purchased from Addgene (#24204, Watertown, MA, USA).

Liu F., Wu Y., Zhang B., Yang S., Shang K., Li J., Zhang P., Deng W., Chen L., Zheng L., Gai X, & Zhang H. (2023). Oncogenic β-catenin-driven liver cancer is susceptible to methotrexate-mediated disruption of nucleotide synthesis. Chinese Medical Journal, 137(2), 181-189.

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Oxaliplatin and PFTα Dissolution Protocol

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Oxaliplatin and PFTα were purchased from TargetMol, America. Oxaliplatin and PFTα were dissolved in DMSO and stored in -20 °C before used.

Zhou Y., Yang L., Xiong L., Wang K., Hou X., Li Q., Kong F., Liu X, & He J. (2021). KIF11 is upregulated in colorectal cancer and silencing of it impairs tumor growth and sensitizes colorectal cancer cells to oxaliplatin via p53/GSK3β signaling. Journal of Cancer, 12(12), 3741-3753.

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Synthesis and Purification of αO-conotoxin GeXIVA

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Synthesis of αO-conotoxin GeXIVA[1,2] was performed as previously described. Gabapentin (T0702, TargetMol) and oxaliplatin (T0164, TargetMol) were purchased from Target molecule Corp.

Wang H., Li X., Zhangsun D., Yu G., Su R, & Luo S. (2019). The α9α10 Nicotinic Acetylcholine Receptor Antagonist αO-Conotoxin GeXIVA[1,2] Alleviates and Reverses Chemotherapy-Induced Neuropathic Pain. Marine Drugs, 17(5), 265.

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Cytotoxic T Cell Activation and Tumor Assay

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T lymphocytes from healthy donors were isolated by Ficoll method (GE Healthcare, Little Chalfont, UK) followed by positive selection using magnetic beads coated with anti-CD8 antibody (Miltenyi Biotec, Bergisch Gladbach, Germany). Cytotoxic CD8⁺ T lymphocytes were stimulated with 500 ng/mL ionomycin (Cayman, Ann Arbor, MI, USA; #1932) and 20 ng/mL phorbol 12-myristate-13-acetate (PMA, Adipogen, San Diego, CA, USA; #AG-CN2-0010) for 24 h. Secretions of mouse and human IFN-γ (interferon gamma) were measured by the respective ELISA kits according to the protocols from the manufacturer (eBioscience, San Diego, CA, USA; #88-7314-88 and #88-7316-88).
B16-OVA tumor cells were exposed to cisplatin or oxaliplatin (TargetMol, Boston, MA, USA) for 24 h and then co-cultured with immune cells (B3Z and bone marrow-derived dendritic cells) as previously described [28] (link). Platins were not removed from the culture media in order to expose immune cells to the drugs. Interleukin-2 (IL-2) levels in the culture supernatants were measured by ELISA (eBioscience, San Diego, CA, USA; #88-7024-88).
Mouse CXCL10 (C-X-C Motif Chemokine Ligand 10) protein levels in culture medium were quantified by ELISA according to the protocol provided by the manufacturer (R&D systems, #DY466-05).

Glorieux C., Xia X., You X., Wang Z., Han Y., Yang J., Noppe G., Meester C.D., Ling J., Robert A., Zhang H., Li S.P., Wang H., Chiao P.J., Zhang L., Li X, & Huang P. (2021). Cisplatin and gemcitabine exert opposite effects on immunotherapy with PD-1 antibody in K-ras-driven cancer. Journal of Advanced Research, 40, 109-124.

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Oxaliplatin and SDZ285-428 Acquisition

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Oxaliplatin and SDZ285-428 were purchased from TargetMol (USA).

Lin W., Zou H., Mo J., Jin C., Jiang H., Yu C., Jiang Z., Yang Y., He B, & Wang K. (2021). Micro1278 Leads to Tumor Growth Arrest, Enhanced Sensitivity to Oxaliplatin and Vitamin D and Inhibits Metastasis via KIF5B, CYP24A1, and BTG2, Respectively. Frontiers in Oncology, 11, 637878.

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Evaluating Oxaliplatin's Impact on 2D and 3D Cancer Cell Growth

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For 3 days, the medium was changed after 600 cells had been seeded in a 6-well plate. Cells were seeded at a density of 2 × 10⁴ per well on a 6-well plate, and after 24 h, the medium was changed with or without Oxaliplatin (OXA, TargetMol, Shanghai, China). After being cultured for 10–14 days, the cells were fixed in 4% paraformaldehyde and stained with crystal violet. Images of the cells taken using a microscope were processed in Image J using an Olympus camera (Tokyo, Japan). For 3D colony formation, 2000 single cells were seeded in 200 µL of culture medium in an ultra-low attachment microplate (7007; Corning, USA) and cultured for 12 days, with medium changes occurring every 3 days with or without OXA. During this time, the tumor spheres were photographed every 4 h using an Incucyte Zoom, and their volumes were calculated (Volume = 4/3πR3).

Jiang Y., Feng Y., Huang J., Huang Z., Tan R., Li T., Chen Z., Tang X., Qiu J., Li C., Chen H, & Yang Z. (2023). LAD1 promotes malignant progression by diminishing ubiquitin-dependent degradation of vimentin in gastric cancer. Journal of Translational Medicine, 21, 632.

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