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73 protocols using 1 octadecene

1

Nanoparticle Synthesis and Characterization

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1-Octadecene, MTT reagent, 4′-6-diamidino-2-phenylindole (DAPI, Thermo Fisher Scientific), acetone (≥99.9%) and acetonitrile (ACN; 99.8%), hexane (95%), iron pentacarbonyl (Fe(CO)5), oleic acid (90%), PTX (Scinopharm), LST (Fluka), PBS (UniRegion Bio-tech), oleylamine (>70%), pluronic F-127 (Sigma-Aldrich Co., St Louis, MO, USA), sodium dodecyl sulfate gel, acrylamide/bis-acrylamide (40% solution, 29:1), N,N,N′,N′-tetramethylethylenediamine (~99%), trimethylamine N-oxide and ammonium persulfate (≥98%) were purchased from Sigma-Aldrich Co. Benzyl ether (99%), gelatin type A (from porcine skin), MMP-2 (≥98%), chloroform, N-hydroxysuccinimide (NHS) and 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide hydrochloride (EDC) were purchased from Sigma-Aldrich Co. Formaldehyde was purchased from Riedel de Haen and Tri-ton X-100 from J.T. Baker. CdSe/ZnS core/shell QDs were obtained from Ocean Nanotech (Springdale, AR, USA). All chemicals and solvents were of analytical reagent grade.
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2

Colloidal Rare-Earth Nanocrystal Synthesis

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Cesium acetate (CsOAc, 99.9%), 1-octadecene (ODE, 90%), oleic acid (OA, 90%), oleylamine (OAm, 70%), lead chloride (PbCl2, 99.99%), europium acetate hydrate (Eu(OAc)3.3H2O, 99.99%), cerium acetate hydrate (Ce(OAc)3.4H2O, 99.99%), erbium acetate hydrate (Er(OAc)3.4H2O, 99.99%), samarium acetate hydrate (Sm(OAc)3.6H2O, 99.99%), potassium acetate (KOAc, 99.9%, ACS grade, Fischer), ethanol (99%), zinc acetate (99.99%), sodium hydroxide (NaOH, 99.99%), toluene (99,95%), were all purchased from Sigma-Aldrich and were used as starting materials without further purification.
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3

Nanoparticle Synthesis and Characterization

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1-Octadecene and oleic acid were purchased from Thermo Fisher Scientific. YCl3 · 6H2O, HAuCl4, OVA, and trypsin were obtained from Sigma (St. Louis, MO, USA). NH4F, ALN, and TPDZ were purchased from Aladdin (Shanghai, China). Fetal bovine serum was provided by Invitrogen (Carlsbad, CA, USA). Sodium bicarbonate, dibasic sodium phosphate, potassium phosphate monobasic, and potassium chloride were purchased from Kemiou (Tianjin, China). Calf serum was obtained from Wisent Canada. Penicillin–streptomycin solution 100X was obtained from Solarbio (Beijing, China). Four to five weeks C57BL/6 J mice (no. 1100111911071516) were obtained from Vital River (Beijing, China).
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4

Synthesis and Characterization of Upconversion Nanoparticles

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Reagent or HPLC grade chemicals used were purchased from Sigma-Aldrich (St Louis, MO, USA): yttrium acetate hydrate, ytterbium acetate hydrate, erbium acetate hydrate, neodymium acetate hydrate, oleic acid, 1-octadecene, sodium hydroxide, ammounium fluoride, ammounium nitrate, ethyl acetate, tetraethyl orthosilicate, chloroform, IR780 iodide, 4-mercaptobenzoic acid, N,N-dimethylformamide, polyallylamine hydrochloride (mw 17000), dimethyl sulfoxide, and cell counting kit 8; from Thermo Fisher Scientific (Waltham, MA, USA): n-hexane, acetone, methanol, and ethanol; from Tokyo Chemical Industry (Tokyo, Japan): n-hexadecyltrimethylammonim chloride (CTAC) ; from Nanocs (New York, NY, USA): FA-PEG-NHS ester (mw 3400). All chemicals were used as received without further purification.
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5

Synthesis of Inorganic Nanomaterials

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All chemicals were used as purchased without further purification. Chromium potassium sulfate dodecahydrate, sodium chloride (99%), potassium hydrogen carbonate (99.7%), potassium ferrocyanide (99%), bismuth powder (99.5%, 100 mesh), copper powder (99.5%, 100 mesh), tin powder (99.5%, 100 mesh), acetone (99%), and 1-octadecene (90% technical grade) were purchased from Thermo Fisher Scientific (Alfa Aesar, Acros Organics). 1,3-Propanediamine-N,N,N′,N′-tetraacetic acid (99%), 1-octanethiol (98.5%), oleylamine (70% technical grade), bismuth neodecanoate, bismuth nitrate pentahydrate (99.99%), ethylene glycol (99%), and molybdenum carbide were purchased from Merck (Sigma Aldrich). Isopropanol (99.5%) was purchased from Honeywell. Potassium hydroxide (reagent grade) was purchased from VWR. Gold mesh (99.9%, 0.06 mm diameter, 20 × 20 mm area, 82 wires per inch) and bismuth foil (99.999%, 0.25 × 10 × 10 mm) were purchased from Goodfellow Cambridge Ltd.
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Synthesis of Cu(NO3)2·3H2O Nanoparticles

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Copper (II) nitride trihydrate 99% (Cu(NO3)2·3H2O) was purchased from Acros Organics; 1-octadecene (90%), oleylamine (90%) and MB were purchased from Thermo Scientific; and 2-propanol was purchased from Honeywell. All chemicals were of analytical grade and used as received without further purification.
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Organic Materials Synthesis and Purification

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For this study, SubPc (99%), mCP (99%), TPBi (99%), C545T (99%), TAPC (99%), and PTB7 (Mw > 50,000) were obtained from Lumtec Inc., C60 (99%) and C70 (99%) were obtained from MER Corporation, MoO3 (99%) and BCP (98%) were obtained from Alfa Aesar, chloroform (99%), 3-mercaptopropionic acid (99%), octane (98%), oleic acid (tech. grade 90%) acetonitrile (99.5%), and methyl acetate (anhydrous) were obtained from Sigma–Aldrich, selenium dioxide (99.8%) and 1-octadecene (90%) were obtained from Acros Organics. All materials above were used as received. The NPD (99%) was synthesized by the Dow Chemical Company and purified once by temperature-gradient sublimation69 (link). Cadmium myristate was prepared on a multi-gram scale according to the method reported by Chen et al.70 (link).
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8

Iron Nanoparticle Synthesis Protocol

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Iron trichloride hexahydrate, hexane, ethanol, and tetrahydrofuran (THF) were purchased from Sinopharm Chemical Reagent Co. Ltd. (Shanghai, China). Sodium oleate was purchased from Beijing InnoChem Science & Technology Co., Ltd. (Beijing, China). Oleic acid was purchased from Alfa Aesar (Shanghai, China). The 1-Octadecene was purchased from Acros Organics (Shanghai, China). Catechol–PEG5000–NH2 was purchased from Ponsure Biotechnology (Shanghai, China). Other reagents were purchased from Sangon Biotech Co. Ltd. (Shanghai, China). All reagents were used without further purification. Ultrapure water (18 MΩ cm−1) was obtained from a Millipore Milli-Q Advantage water purification system (Burlington, MA, USA). E. coil BL21 (DE3) and XL1-Blue cells were purchased from TransGen Biotech Co. Ltd. (Beijing, China).
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9

Synthesis and Characterization of Cobalt Ferrite Nanoparticles

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Cobalt chloride (II) hexahydrate (ACS reagent, 98%), iron chloride (III) hexahydrate (ACS reagent, 97%), oleic acid (90%), hexane (ACS reagent, ≥98.5%), diethyl ether (anhydrous, ACS reagent, ≥99%), toluene (ACS reagent, ≥99.5%), and chloroform (for high-performance liquid chromatography, gas chromatography, and residue analysis, ≥99.9%) were purchased from Sigma-Aldrich. 1-Octadecene (90%) was purchased from Acros Organics, and sodium oleate (>97%) was purchased from TCI America. PEG, 5 kDa for ligand exchange, was procured from Sigma-Aldrich, and block copolymer polyethylene glycol-b-poly(lactic acid) PEG4.9kDa-b-PLA6.0kDa was purchased from Evonik Industries. Tetrahydrofuran (THF; 99.9% Acros Organics) was dried using sodium sulfate before use in Flash Nanoprecipitation (FNP). HA sodium salt from Streptococcus equi was purchased from Sigma-Aldrich. Bovine synovial fluid was obtained from Animal Technologies. Water was used from Milli-Q grade (Millipore, Bedford, MA).
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10

Synthesis and Characterization of Colloidal Nanoparticles

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Chemicals listed were purchased include Cadmium Oxide-99% (CdO), Zinc Acetate-99% (Zn(Act)2), Oleic Acid (OA), Sulfur powder-99% (S), Diphenylphosphine 98% (DPP), Trioctylphosphine 97% (TOP), Tetra-methyl-ammonium-hydroxide Solution (TMAH), N-hydroxy-succinimide-98% (NHS), and NH2-(PEG)8-Propionic acid from Sigma-Aldrich. 1-Octadecene-90% (ODE) from Acros Organics. Chloroform, Acetone, Methanol, and Isopropanol (ISP) from J.T.Baker. 3-Mercaptopropionic acid ≥ 99% (MPA) from Merckmillipore. 1-ethyl-3-[3-dimethylaminopropyl] carbodiimide-98% (EDC) from Tokyo Chemical Industry Co. And Streptavidin (SA) from Alfa Aesar, Cell culture reagents include Dulbecco’s modified Eagle’s Medium (DMEM), Roswell Park Memorial Institute-1640 medium (RPMI-1640), Fetal bovine serum (FBS), 1 mg/mL glutamine, and 100 μg/mL Streptomycin/Penicillin solution, from Gibco Bio Sciences. 3-(4,5-Dimethylthiazol-2-yl)-2,5-Diphenyl tetrazolium Bromide (MTT) and Sodium lauryl sulfate (SDS) from thermo scientific and other related chemicals are further mentioned. .
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