Cells were fixed with 4% paraformaldehyde and then incubated in immunol staining blocking buffer (1× PBS/5% BSA/0.3% Triton X‐100) for 2 hours to permeabilize the cells and block non‐specific protein–protein interactions. Cells were then incubated with anti‐galectin‐3 antibody (Abcam) or anti‐HER2 antibody (Abcam) followed by Alexa Fluor 594‐secondary antibody (Proteintech). Nuclei were detected using 4′,6‐diamidino‐2‐phenylindole (DAPI) staining. Cells were then examined using a fluorescence microscope.
Anti galectin 3 antibody
Manufactured by Abcam
Sourced in United Kingdom
Anti‐galectin‐3 antibody is a laboratory reagent used to detect and study the galectin‐3 protein. Galectin‐3 is a member of the lectin family of proteins and is involved in various biological processes. This antibody can be used in techniques such as Western blotting, immunohistochemistry, and immunocytochemistry to identify and analyze the expression and localization of galectin‐3 in samples.
Automatically generated - may contain errors
Lab products found in correlation
Anti her2 antibody, by Abcam (1 mentions)
Alexa fluor 594 secondary antibody, by Proteintech (1 mentions)
Chloroquine, by Merck Group (1 mentions)
Anti cathepsin b antibody, by Abcam (1 mentions)
Normal rabbit igg antibody, by Cell Signaling Technology (1 mentions)
Alexa 488, by Thermo Fisher Scientific (1 mentions)
Dimethyl sulfoxide (dmso), by Merck Group (1 mentions)
2 protocols using anti galectin 3 antibody
1
Immunolabeling of Galectin-3 and HER2
2
Investigating Alzheimer's Pathways with Antibodies
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Anti-Aβ 6E10 and 4G8 antibodies were purchased from BioLegend (San Diego, CA, USA), anti-APP C-terminus antibody was from Sigma-Aldrich (St. Louis, MO, USA), anti-Cathepsin B antibody was from Abcam (Cambridge, UK), anti-Galectin 3 antibody was from Abcam, anti-LAMP1 antibodies were from Sigma-Aldrich and Abcam, anti-nicastrin antibody was from Novus Biologicals (Littleton, CO, USA), and anti-V-ATPase antibody was from Abcam. Normal rabbit IgG antibody was purchased from Cell Signaling Technology, Inc. (Danvers, MA, USA), whilst Alexa488 and Cy3-conjugated donkey anti-mouse/rabbit IgG were from Thermo Fisher Scientific (Waltham, MA, USA) and Jackson ImmunoResearch Inc. (West Grove, PA, USA), respectively. DAPT, γ-secretase inhibitor, was purchased from Abcam. DMSO and Chloroquine were purchased from Sigma-Aldrich.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!