AHSA1, N-cadherin, 6-his, vimentin, HSP90, MEK1/2, E-cadherin, and CALD1 antibodies were obtained from Proteintech (Wuhan, China). ERK1/2, Phospho-MEK1/2, and Phospho-ERK1/2 antibodies were obtained from Cell Signaling Technology (Beverly, MA, USA). Ki67 antibody was obtained from Abcam (Cambridge, MA, USA). Phospho-CALD1 antibody was purchased from Thermo Fisher Scientific (Waltham, MA, USA). β-tubulin antibody was obtained from Bioworld Technology (Bloomington, MN, USA).
Cald1
Manufactured by Proteintech
Sourced in China
CALD1 is a protein-coding gene that is involved in the regulation of calcium-dependent processes in cells. The CALD1 gene encodes the caldesmon protein, which plays a role in the organization and dynamics of the cytoskeleton.
Automatically generated - may contain errors
Lab products found in correlation
E cadherin, by Proteintech (2 mentions)
Ki67 antibody, by Abcam (1 mentions)
N cadherin, by Proteintech (1 mentions)
Vimentin, by Proteintech (1 mentions)
Hsp90, by Proteintech (1 mentions)
Phospho mek1 2, by Cell Signaling Technology (1 mentions)
6 his, by Proteintech (1 mentions)
Mek1 2, by Proteintech (1 mentions)
Phospho erk1 2, by Cell Signaling Technology (1 mentions)
Erk1 2, by Cell Signaling Technology (1 mentions)
Fitc conjugated secondary antibody, by Thermo Fisher Scientific (1 mentions)
Snai1, by Proteintech (1 mentions)
Fetal bovine serum (fbs), by Thermo Fisher Scientific (1 mentions)
Lipofectamine 2000 lipo 2000 reagent, by Thermo Fisher Scientific (1 mentions)
Polyvinylidene difluoride pvdf membrane, by Merck Group (1 mentions)
Dulbecco s modified eagle medium dmem, by Thermo Fisher Scientific (1 mentions)
Rpmi 1640, by Thermo Fisher Scientific (1 mentions)
Enhanced chemiluminescence reagent, by Thermo Fisher Scientific (1 mentions)
Horseradish peroxidase conjugated secondary antibody, by Thermo Fisher Scientific (1 mentions)
3 protocols using cald1
1
Antibody Panel for Cell Signaling Analysis
2
Immunocytochemistry Analysis of EMT Markers
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As previously explained (Yang et al., 2017 (link)), immunocytochemistry was carried out following exposure of HCT116 cells to cisatracurium. In summary, HCT116 cells were incubated in PBS containing 4% para-formaldehyde at 25°C for 20 min. For permeabilization, HCT116 cells were further maintained in PBS containing 0.5% Triton X-100 at 4°C for 10 min. After blocking using 3% BSA reagent, cells were exposed to primary antibodies for SLUG, SNAI1, E-Cadherin, and CALD1 (Proteintech). FITC conjugated secondary antibody (Invitrogen) was added and then stained with 1 μg/ml of DAPI for 60 s. Images of samples were visualized and captured using a fluorescence microscope (Olympus BX83, Japan).
3
Molecular Pathway Profiling in Cells
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RPMI-1640, Dulbecco’s Modified Eagle Medium (DMEM), and fetal bovine serum (FBS) were obtained from GIBCO (Grand Island, NY). Lipofectamine 2000 (Lipo2000) reagent was obtained from Invitrogen (Life Technologies Corporation, Carlsbad, CA). The primers for miR-320a and U6 real-time PCR, miR-320a mimics, miR-320a inhibitor, and their controls were purchased from RiboBio (Guangzhou, China). Antibodies against MafF (Cat No: 12771-1-AP) and CALD1 (Cat No: 66693-1-lg) were procured from Proteintech (Chicago, MA). Antibodies against DDRGK1 (Cat No: bs-8252R) and KLHL36 (Cat No: bs-9628R) were purchased from Bioss (Beijing, China). Antibodies against AKT (Cat No: 4691T) and p-AKT (Cat No: 13038T) were purchased from CST (Cell Signaling Technology, Shanghai, China). Polyvinylidene difluoride (PVDF) membranes were purchased from Millipore (Merck KGaA, Darmstadt, Germany). Horseradish peroxidase-conjugated secondary antibodies and enhanced chemiluminescence reagents were obtained from Pierce Biotechnology (Thermo Fisher Scientific Inc., Rockford, IL). Other reagents were purchased from the Sigma-Aldrich Company, unless otherwise specified.
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