Radioimmunoprecipitation assay (RIPA) buffer was used for protein extraction. The supernatants from cell lysates were run on 10% acrylamide gels by SDS-PAGE and then transferred to a polyvinylidene difluoride membrane (Millipore). Antibodies against ROCK2 (1:1000, #ab71598, Abcam), LC3 (1:1000, #12741, Cell Signaling Technology), p62 (1:1000, #ab56416, Abcam), and Beclin1 (1:1000, #ab210498, Abcam) and an HRP-conjugated secondary antibody (1:2000) were employed for western blotting. A chemiluminescence western blotting detection system (Bio-Rad) was used for protein detection.
Chemiluminescence western blotting detection system
Manufactured by Bio-Rad
The Chemiluminescence Western Blotting Detection System is a lab equipment product that enables the detection and visualization of proteins separated by Western blotting. The system utilizes chemiluminescent substrates to generate a light signal that is captured and quantified, allowing for the identification and analysis of target proteins.
Automatically generated - may contain errors
Lab products found in correlation
Ab71598, by Abcam (1 mentions)
Ab210498, by Abcam (1 mentions)
Ab56416, by Abcam (1 mentions)
Polyvinylidene difluoride membrane, by Merck Group (1 mentions)
Ab186738, by Abcam (1 mentions)
Ab45956, by Abcam (1 mentions)
Polyvinylidene fluoride membrane, by Merck Group (1 mentions)
Ab8245, by Abcam (1 mentions)
2 protocols using chemiluminescence western blotting detection system
1
Western Blot Analysis of Autophagy Markers
2
Western Blot Optimization for Protein Detection
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The protein was extracted by Radioimmunoprecipitation assay (RIPA) buffer. The supernatant of cell lysates was run on a 10% acrylamide gel by SDS-PAGE and then transferred to a polyvinylidene fluoride membrane (Millipore). Anti-NFIB (1:1,000, #ab186738, Abcam), Anti-ASS1 (1:1,000, #16210-1-AP, proteintech), Anti-CPS1 (1:1,000, #ab45956, Abcam) and Anti-GAPDH (1:1,000, #ab8245, Abcam) were combined with HRP-conjugated secondary antibody (1:1,000) for western blotting. A chemiluminescence western blotting detection system (Bio-Rad) was used for protein detection.
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