To measure the amount of secreted ALB, the medium was collected 24 h after changing and analyzed using a human ALB ELISA kit (catalog number: E80-129; Bethyl Laboratories), per the manufacturer’s instructions. The absorbance was measured using a microplate reader (S1LFA; BioTek Instruments, Inc., Winooski, VT, USA), and the data were adjusted for the number of cells.
S1lfa
Manufactured by Agilent Technologies
Sourced in United States
The S1LFA is a high-performance laboratory instrument designed for advanced liquid flow analysis. It provides precise and reliable measurements of liquid flow parameters, supporting a wide range of applications in research and development, as well as industrial settings. The core function of the S1LFA is to accurately measure and analyze the flow characteristics of liquids.
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Lab products found in correlation
3 protocols using s1lfa
1
Quantifying Secreted Albumin via ELISA
2
Quantifying Alpha-1 Antitrypsin Levels
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To quantify the level of alpha-1 antitrypsin (AAT), the culture medium was collected 24 h after replacement with fresh medium and analyzed using a human AAT ELISA quantitation kit (catalog number: EA5101-8; Assaypro, St. Charles, MO, USA), following the manufacturer’s instructions. The absorbance was measured using a microplate reader (S1LFA; BioTek Instruments, Inc.), and the results were normalized to the cell number.
3
Oleic Acid Uptake in RAW264.7 Cells
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Fatty acid accumulation in RAW264.7 cells was measured by exposing the cells to oleic acid (OA), following the previously described method (19 (link)). RAW264.7 cells were exposed to 0.1 mM OA in the presence or absence of GlcN, galactosamine (GalN), mannosamine (ManN) or LPS for 24 hrs. Cells were subsequently washed and stained with 0.3% ORO working solution at RT for 15-30 min.
The rate of fatty acid uptake was determined using green fluorescent fatty acid, 4,4-difluoro-5,7-dimethyl-4-bora-3α,4α-diaza-s-indacene-3-hexadecanoic acid (BODIPYTM FL C16, Thermo Fisher Scientific, MA, USA). Cells were treated with LPS and/or GlcN for 12 hrs, washed twice with PBS, and incubated with 1 μg/ml BODIPYTM FL C16 for 3 min at RT. After an four washes, the fluorescence/well was measured (S1LFA; Biotek, VT, USA).
The rate of fatty acid uptake was determined using green fluorescent fatty acid, 4,4-difluoro-5,7-dimethyl-4-bora-3α,4α-diaza-s-indacene-3-hexadecanoic acid (BODIPYTM FL C16, Thermo Fisher Scientific, MA, USA). Cells were treated with LPS and/or GlcN for 12 hrs, washed twice with PBS, and incubated with 1 μg/ml BODIPYTM FL C16 for 3 min at RT. After an four washes, the fluorescence/well was measured (S1LFA; Biotek, VT, USA).
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