Dulbecco s modified eagle medium dmem

Manufactured by PanEco

Dulbecco's Modified Eagle Medium (DMEM) is a cell culture medium commonly used to support the growth of a variety of cell types in vitro. It is a balanced salt solution that provides essential nutrients, vitamins, and other components required for cell maintenance and proliferation.

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Lab products found in correlation

Mtt reagent, by Merck Group (1 mentions) Dimethyl sulfoxide (dmso), by Merck Group (1 mentions) Phosphate buffered saline (pbs), by PanEco (1 mentions) Stearoyl chloride, by Merck Group (1 mentions) Bortezomib, by Santa Cruz Biotechnology (1 mentions) Prothionamide, by Merck Group (1 mentions) Trypsin versene solution, by PanEco (1 mentions) Phosphatidylcholine, by Avanti Polar Lipids (1 mentions) Doxorubicin, by Merck Group (1 mentions) Trypsin edta solution, by PanEco (1 mentions) Hanks solution, by PanEco (1 mentions) L glutamine, by PanEco (1 mentions) Gentamicin, by PanEco (1 mentions)

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DMEM medium (29 citations) Dulbecco’s modified (2 citations)

2 protocols using dulbecco s modified eagle medium dmem

Synthesis and Evaluation of Biotargeted Compounds

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AIBN (2,2'-azobisisobutyronitrile), 1,4-dioxane and VP (N-vinyl-2-pyrrolidone) were obtained from Acros (https://www.thermofisher.com/ru/en/home/chemicals/acros-organics.html). Stearoyl chloride, DMSO (dimethylsulfoxide), potassium tert-butylate, prothionamide and MTT reagent (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl-2H-tetrazolium bromide) were purchased from Sigma-Aldrich; Merck KGaA. Bortezomib was obtained from Santa Cruz Biotechnology, Inc. Dulbecco's Modified Eagle Medium (DMEM), trypsin-versene solution and PBS were purchased from PanEco. Fetal bovine serum (FBS) was purchased from HyClone; Cytiva. Cyclo-RGDfK(TPP) peptide was a kind gift of Professor S. Burov (Cytomed JSC, St-Petersburg, Russia).

Yagolovich A.V., Kuskov A.N., Kulikov P.P., Bagrov D.V., Petrova P.A., Kukovyakina E.V., Isakova A.A., Khan I.I., Pokrovsky V.S., Nosyrev A.E., Stamati P.C., Markvicheva E.A., Gasparian M.E., Spandidos D.A, & Tsatsakis A.M. (2024). Assessment of the effects of amphiphilic poly (N‑vinylpyrrolidone) nanoparticles loaded with bortezomib on glioblastoma cell lines and zebrafish embryos. Biomedical Reports, 20(3), 37.

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In Vitro Cytotoxicity Assay

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BA, propidium iodide (PI), fetal bovine serum (FBS), human insulin, and doxorubicin were purchased from Sigma-Aldrich (St. Louis, MO, USA). Dulbecco’s Modified Eagle Medium (DMEM) containing 1 g/L glucose, 25 mM HEPES, sodium bicarbonate; L-glutamine; gentamicin; Hank’s solution; and 0.25% trypsin-EDTA solution were purchased from Paneco (Moscow, Russia). Phosphatidylcholine was purchased from Avanti Polar Lipids (Avanti Polar Lipids Inc., Alabaster, AL, USA). The conjugate and BA used in this study were prepared in DMSO as a 5–10 mM stock solution.

Belosludtsev K.N., Ilzorkina A.I., Belosludtseva N.V., Sharapov V.A., Penkov N.V., Serov D.A., Karagyaur M.N., Nedopekina D.A., Davletshin E.V., Solovieva M.E., Spivak A.Y., Kuzmina U.S., Vakhitova Y.V., Akatov V.S, & Dubinin M.V. (2022). Comparative Study of Cytotoxic and Membranotropic Properties of Betulinic Acid-F16 Conjugate on Breast Adenocarcinoma Cells (MCF-7) and Primary Human Fibroblasts. Biomedicines, 10(11), 2903.

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