Human 8 ohdg elisa kit

Human Oxidized LDL (MDA-LDL Quantitation) (Oxiselect, cod. STA-369, Cell Biolabs, Inc), Human 3-nitrotyrosine (3-NT) ELISA kit (cod. CSB-E14324H, Cusabio Biotech Co), Human 8-OHdG ELISA Kit (cod. CSB-E10140H, Cusabio Biotech Co), Human advanced glycation end-products (AGE) kit (cod. CSB-E14324H, Cusabio Biotech Co) were used according to manual or data sheet.

Placental tissue, cell, and serum samples were collected. Total protein was extracted, and its content was determined using the BCA kit. The human 8-OHdG ELISA kit was obtained from CUSABIO (China). Levels of 8-OHdG were determined according to the manufacturer’s instructions. A microplate reader was operated to determine the OD value.

SH-SY5Y cells were purchased from the Institute of Basic Medical Sciences of Chinese Academy of Medical Sciences (Guangzhou, Guangdong, China). High-glucose Dulbecco’s modified Eagle’s medium (DMEM), fetal calf serum (FBS) and trypsin-EDTA solution were purchased from Gibco (Grand Island, NY, USA). Cell culture plates were purchased from Jet Biofil (Guangzhou, Guangdong, China). MDHB (No. L09552) was obtained from the Alfa Aesar Co. (Ward Hill, MA, USA). MTT, DMSO and DPPH-free radicals were purchased from Sigma (St. Louis, MO, USA). TBHP (No. B106035) was purchased from Aladdin (Shanghai, China). Malonyldialdehyde (MDA), LDH, GSH-Px and superoxide dismutase (SOD) assay kits were obtained from Nanjing Jiancheng Bioengineering Institute (Nanjing, Jiangsu, China). The Apoptosis Detection Kit, Reactive Oxygen Species Assay Kit and BCA Protein Assay Kit were obtained from the BiYunTian Biotech Company (Shanghai, China). The human 8-OHdG ELISA kit was obtained from Cusabio (Barksdale, DE, USA). Antibodies against Akt, phospho-Akt and Bcl-2, Bax, caspase-9, and caspase-3 were purchased from Cell Signaling Technology (Boston, MA, USA).