Goat anti human il 33

Manufactured by R&D Systems

Goat anti-human IL-33 is a polyclonal antibody produced in goats and purified from goat serum. It is reactive with human interleukin-33 (IL-33), a cytokine that belongs to the IL-1 family and plays a role in the activation of various immune cells.

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Lab products found in correlation

Goat anti mouse il 33, by R&D Systems (1 mentions) Goat anti mouse il 1α, by R&D Systems (1 mentions) Goat anti rabbit igg secondary antibody hrp, by Thermo Fisher Scientific (1 mentions) Westernsure premium reagent, by LI COR (1 mentions) Duoset kit, by R&D Systems (1 mentions) Ae1 ae3, by Thermo Fisher Scientific (1 mentions) Hc pl apo cs2 63 1.40 oil objective, by Leica Microsystems (1 mentions) Mouse anti human vimentin, by Cell Signaling Technology (1 mentions) Mouse anti pan cytokeratin, by Cell Signaling Technology (1 mentions) Tcs sp8 confocal microscope, by Leica camera (1 mentions) Las x software, by Leica Microsystems (1 mentions) Rat anti cd31, by BD (1 mentions)

3 protocols using goat anti human il 33

Quantification of IL-33 and IL-1α by ELISA and Western Blot

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R&D Systems Duoset kits were used to measure human and murine IL-33 by ELISA, while western blotting was carried out using polyclonal goat anti-mouse IL-33, goat anti-human IL-33 or goat anti-mouse IL-1α (R&D Systems) with a rabbit anti-goat IgG HRP secondary antibody (Thermo Fisher), and detected using WesternSure Premium reagent (Licor).

Osbourn M., Soares D.C., Vacca F., Cohen E.S., Scott I.C., Gregory W.F., Smyth D.J., Toivakka M., Kemter A.M., le Bihan T., Wear M., Hoving D., Filbey K.J., Hewitson J.P., Henderson H., Gonzàlez-Cìscar A., Errington C., Vermeren S., Astier A.L., Wallace W.A., Schwarze J., Ivens A.C., Maizels R.M, & McSorley H.J. (2017). HpARI Protein Secreted by a Helminth Parasite Suppresses Interleukin-33. Immunity, 47(4), 739-751.e5.

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Immunostaining of Endometriotic Lesions

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Frozen endometriotic lesions were embedded in optimal cutting temperature compound and sectioned at a 5 μm thickness. Then, the sections were washed with PBS, permeabilized with 0.2% Triton X-100, washed with PBS for 10 minutes, and blocked with 10% FBS for 1 hour. The sections were then incubated with primary antibodies overnight at 4°C, washed with PBST, and then, where required, stained with Alexa Fluor–conjugated secondary antibodies for 1 hour at room temperature. The following primary antibodies were used: Goat Anti-human IL-33 (1:40, R&D Systems, AF3625), Mouse Anti-human Vimentin (1:400, Cell Signaling Technology, clone D21H3), Mouse Anti-Pan cytokeratin (1:40, Cell Signaling Technology, clone AE1/AE3), Rabbit Anti-goat Alexa Fluor 647 (1:200; Invitrogen, A27018). Immunostained tissue sections were imaged using the Leica TCS SP8 confocal microscope. Images were taken in a Z-stack (1.20 μm; Z dimension) and tile scan (12 tiles, 580 μm × 435 μm each tile; x and y dimensions) acquisition mode. Two to 3 fields per preparation were imaged using HC PL APO CS2 ×63/1.40 oil objective and LAS-X Software (Leica Microsystems).

Miller J.E., Lingegowda H., Symons L.K., Bougie O., Young S.L., Lessey B.A., Koti M, & Tayade C. (2021). IL-33 activates group 2 innate lymphoid cell expansion and modulates endometriosis. JCI Insight, 6(23), e149699.

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Immunostaining Techniques for Neuronal and Immune Markers

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Primary antibodies used for immunostaining were rabbit anti-TH (Millipore catalog no. AB152, RRID: AB_390204), rabbit anti-STMN2 (Novus Biologicals catalog no. NBP1-49461, RRID: AB_10011568), mouse anti-Tuj1 (BioLegend catalog no. 801202, RRID: AB_10063408), rabbit anti-Tuj1 (Cell Signaling Technology catalog no. 5568, RRID: AB_10694505), rabbit anti-PGP9.5/UCHL1 (Proteintech catalog no. 14730–1-AP, RRID: AB_2210497), goat anti-VAChT (Millipore catalog no. ABN100, RRID: AB_2630394), rabbit anti-UCP1 (Abcam catalog no. ab10983, RRID: AB_2241462), goat anti-mouse IL-33 (R&D Systems catalog no. AF3626, RRID: AB_884269), goat anti-human IL-33 (R&D Systems catalog no. AF3625, RRID: AB_1151900), mouse anti-human Siglec-8 (BioLegend catalog no. 347102, RRID: AB_2239311), rat anti-Siglec-F-PE (BD Biosciences catalog no. 552126, RRID: AB_394341), rat anti-mouse CD45-PE (BioLegend catalog no. 103106, RRID: AB_312971), rat anti-human CD45-FITC (BioLegend catalog no. 368508, RRID: AB_2566368), rat anti-CD31 (BD Biosciences catalog no. 553370, RRID: AB_394816). In addition, Alexa Fluor dye–conjugated secondary antibodies were from Thermo Fisher Scientific. Anti–IL-5 neutralizing antibody was from BioXCell (Bio X Cell, catalog no. BE0198, RRID: AB_10950522), and the IgG isotype control was from BioXCell (Bio X Cell, catalog no. BE0083, RRID: AB_1107784).

Meng X., Qian X., Ding X., Wang W., Yin X., Zhuang G, & Zeng W. (2022). Eosinophils regulate intra-adipose axonal plasticity. Proceedings of the National Academy of Sciences of the United States of America, 119(3), e2112281119.

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