Olaparib, AZD-2461, A-966492, niraparib, veliparib, rucaparib, talazoparib, and iniparib were obtained from Selleck and stock solutions (2 mM) were prepared in DMSO. Fluorescent Olaparib (fl-Ola) was obtained from Tocris and a stock solution (10 µM) was prepared in DMSO. The p18mer* DNA oligonucleotides and its complementary strand were obtained from IDT: p18mer: 5′-phosphate-5′-fluorescein-GGGTTGCGGCCGCTTGGG-3′. Nucleosome with overhanging ends of 7 and 11 bp was prepared using the Widom601 sequence as previously described40 (link). Wild-type PARP1 and PARP2 were expressed and purified as previously described41 (link),42 . Purification of HPF1 to homogeneity was performed essentially as previously described25 (link), with the slight modification of using a gradient for the nickel affinity column from 0 to 40% over 15 CV. The stability of HPF1 was evaluated using the Thermo Fisher Protein Thermal Shift kit according to manufacturer’s instructions.
Iniparib
Manufactured by Selleck Chemicals
Sourced in Germany
Iniparib is a chemical compound used as a laboratory reagent. It is a poly(ADP-ribose) polymerase (PARP) inhibitor, primarily employed in research and scientific experiments.
Automatically generated - may contain errors
Lab products found in correlation
Veliparib, by Selleck Chemicals (2 mentions)
Olaparib, by Selleck Chemicals (2 mentions)
Niraparib, by Selleck Chemicals (1 mentions)
Rucaparib, by Selleck Chemicals (1 mentions)
Talazoparib, by Selleck Chemicals (1 mentions)
Protein thermal shift kit, by Thermo Fisher Scientific (1 mentions)
Azd2461, by Selleck Chemicals (1 mentions)
A 966492, by Selleck Chemicals (1 mentions)
Gsk343, by Selleck Chemicals (1 mentions)
Ldc000067, by Selleck Chemicals (1 mentions)
Rg108, by Selleck Chemicals (1 mentions)
Gsk j4, by Selleck Chemicals (1 mentions)
I bet151, by Selleck Chemicals (1 mentions)
Unc0638, by Selleck Chemicals (1 mentions)
Ab1838, by Abcam (1 mentions)
3 4 5 dimethylthiazol 2 yl 2 5 diphenyltetrazolium bromide mtt propidium iodide, by Merck Group (1 mentions)
Sc 20696, by Santa Cruz Biotechnology (1 mentions)
3 protocols using iniparib
1
PARP Inhibitor Characterization Protocol
2
Synthesis and Characterization of Small Molecule Inhibitors
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3
Investigating DNA Repair Modulator Effects
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Trabectedin (Yondelis®) was manufactured by PharmaMar S.A. (Madrid, Spain). Veliparib, olaparib, and iniparib were purchased from Selleck Chemicals (Munich, Germany). Stock solutions of drugs were prepared in pure DMSO at the appropriate concentrations and stored at -20℃ until use. Propidium iodide (PI), 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT), and antibodies against α-tubulin (T5168) were obtained from Sigma (St. Louis, USA). Antibodies against FEN1 (ab17993), DNA Pol β (ab26343), XRCC1 (ab1838), FANCD2 (ab2187), and ATM (Y170) were obtained from Abcam (Cambridge, UK). Antibodies against DNA-PK (#4602) and BRCA1 (#9010) were obtained from Cell Signaling Technologies (Danvers, USA). Antibodies against PARP-1 (sc-7150) and XPD (sc-20696) were obtained from Santa Cruz Biotechnology (Santa Cruz, USA). Antibodies against γ-H2AX (05-636), PAR (#551813), XPF (MS-1381), and XPG (A301-484A) were obtained from Merck Millipore (Billerica, USA), BD Pharmigen (San Jose, USA), NeoMarkers (Fremont, USA), and Bethyl Lab (Montgomery, USA), respectively.
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