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Pancreatic lipase

Manufactured by Yuanye Bio-Technology
Sourced in China

Pancreatic lipase is an enzyme produced by the pancreas that plays a key role in the digestion of dietary fats. It catalyzes the hydrolysis of triglycerides into monoglycerides and free fatty acids, facilitating their absorption by the body.

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4 protocols using pancreatic lipase

1

Tibetan Barley Compositional Analysis

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HB was obtained from Lhasa, Tibet. Acetic acid ≥98%, propionic acid ≥99%, butyric acid ≥99%, valeric acid ≥99%, and 2-ethylbutyric acid ≥98% were obtained from Aladdin Bio-Chem Technology Co., Ltd., (Shanghai, China). Total glucose oxidase/peroxidase (GOPOD) kit, β-glucan (mixed linkage) kit, total dietary fiber kit, and total starch kit were obtained via Megazyme International Ireland, Ltd. (Bray Co., Wicklow, Ireland). α-amylase from porcine pancreas type VI-B (A3176, 11 units/mg) and pepsin from porcine gastric mucosa (P7125, ≥400 units/mg) were obtained via Sigma Chemical Co. (St. Louis, Mo., USA). Pancreatic enzyme (S10031, trypsin ≥4000 units/g, pancreatic α-amylase ≥7000 units/g, and pancreatic lipase ≥4000 units/g) was obtained via the Shanghai yuanye Bio-Technology Co. (Shanghai, China). Other chemicals and reagents used were of analytical grade.
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2

Akebia trifoliata Fruit Phytochemicals

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Akebia trifoliata fruits were sourced from a local plantation in Zunyi city, Guizhou, China. Cellulase (15,000 μ/g), α-amylase (40,000 μ/g), papain (800,000 μ/g), α-glucosidase, pepsin, pancreatic lipase, 1,1-diphenyl-2-picrylhydrazine (DPPH), 2,2′-azinobis (3-ethylbenzothiazoline-6-sulfonic acid) (ABTS), 2,4,6-tripyridyltriazine (TPTZ), p-nitrophenyl-α-D-glucopyranoside (PNPG) and cholesterol were purchased from Shanghai Yuanye Bio-Technology Co., Ltd., Shanghai, China. Monosaccharide standards were obtained from Sigma Chemical Co., St. Louis, MO, USA. All other chemicals and reagents of analytical grade were obtained from Sinopharm Chemical Reagent, Shanghai, China.
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3

Fabrication of Zirconium-based Composite Materials

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Ferric chloride hexahydrate (FeCl3·6H2O; AR, 99%), sodium acrylate (CH2=CHCOONa; 95%), ZrCl4 (99.5%), aminoterephthalic acid (ATA; 98%), N, N-dimethyl-formamide (DMF; AR, 99.5%), ethylene glycol (EG; AR, 98%), anhydrous sodium acetate (CH3COONa; AR, 99%), coomassie brilliant blue G-250 (AR, 98%), p-nitrophenol (AR), and p-nitrophenyl butyrate (p-NPB; 98%) were purchased from Shanghai Macklin Biochemical Co., Ltd., (Shanghai, China). In addition, 50% glutaraldehyde (GA), acetic acid (HAc), ethanol absolute, and ammonium sulfate were of analytical grade and purchased from Shanghai Aladdin Biochemical Technology Co., Ltd., (Shanghai, China). Pancreatic lipase (EC.3.1.1.3 from porcine pancreas) and bovine albumin (BSA; 98%) were provided by Shanghai Yuanye Biotechnology Co., Ltd., (Shanghai, China). S. baicalensis extract was purchased from Beijing Kangrentang Pharmaceutical Co., Ltd., (Beijing, China). All chemicals and reagents were obtained from commercial sources and used without any further purification.
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4

Quinoa Cultivation and Phytochemical Analysis

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Dehulled quinoa cultivar ‘Jili 3’ was grown and harvested at Zhangjiakou Yuerwan Quinoa Planting Cooperative, which is located in Zhangjiakou City (115°36′ E, 415°27′ N), Hebei Province, China. The seeds were preserved in the dark at −20 °C under vacuum until germination. GABA, with a purity ≥ 99%, and 2,2-diphenyl1-picrylhydrazyl (DPPH) were purchased from Sigma-Aldrich (Shanghai, China). Threonine (99%), valine (99%), methionine (98%), isoleucine (98%), leucine (98%), phenylalanine (98%), lysine (98%), histidine (98%), arginine (98%), aspartate (98%), serine (98%), glutamate (98%), glycine (98%), alanine (98%), cysteine (98%), tyrosine (99%), proline (98%), gallic acid (99%), rutin (95%), saponin (98%), 2,2′-azino-bis (3-ethylbenzthiazoline-6-sulfonic acid) (ABTS) (98%), sodium cholate (65%), sodium taurocholate (98%), sodium deoxycholate (98%), and sodium glycocholate (97%), as well as α-amylase (100,000 U/g), α-glucosidase (50,000 U/g), and pancreatic lipase (30,000 U/g), were obtained from Shanghai Yuanye Bio-Technology Co., Ltd. (Shanghai, China). Acetonitrile (chromatographically pure) was purchased from Macklin Biochemical Co. Ltd. (Shanghai, China). All other chemicals were analytical reagent grade.
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