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1 1 1 3 3 3 hexafluoroisopropanol hfip

Manufactured by Merck Group
Sourced in United States

1,1,1,3,3,3-hexafluoroisopropanol (HFIP) is a fluorinated organic compound with the chemical formula (CF3)2CHOH. It is a colorless, dense liquid with a high boiling point. HFIP is commonly used as a solvent in various laboratory applications due to its unique chemical properties.

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12 protocols using 1 1 1 3 3 3 hexafluoroisopropanol hfip

1

Bioinspired Composite Scaffold Fabrication

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Poly(l-lactic-co-glycolic acid), with an LA/GA ratio of 75 : 25 and a molecular weight of 150 000 g mol−1, was purchased from SinoBiomaterials Co.,Ltd. GO was purchased from Chengdu Organic Chemicals Co. Ltd, China (thickness: 0.55–1.2 nm, diameter: 0.5–3 μm) and HA powder was obtained from the Changchun Institute of Applied Chemistry, Chinese Academy of Sciences (CIAC, China). bFGF was purchased from Peprotech, USA. BMP-2 was purchased from UB Biotech. HA powder was obtained from Nanjing Emperor Nano Material Co., Ltd. 1,1,1,3,3,3-hexafluoroisopropanol (HFIP) and 3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2-H-tetrazolium bromide (MTT) were purchased from Sigma-Aldrich (USA).
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2

PLGA-IGF1-GO Nanocomposite Protocol

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Poly(lactide-co-glycolide) (PLGA, Mw = 70 000 g mol−1) with a lactic acid–glycolic acid ratio of 80 : 20 was purchased from Changchun Sino Biomaterials Co., Ltd, China. IGF-1 was purchased from PeproTech (USA). GO was purchased from Chengdu Organic Chemicals Co., Ltd., China (thickness: 0.55–1.2 nm; diameter: 0.5–3 μm). 3-(4,5-Dimethyl-2-thiazolyl)-2,5-diphenyl-2-H-tetrazolium bromide (MTT) and 1,1,1,3,3,3-hexafluoroisopropanol (HFIP) were purchased from Sigma-Aldrich (USA). Cetylpyridinium chloride (CPC) was purchased from Aladdin Chemistry Co., Ltd, China. NSC culture media components were purchased from Gibco (USA). Reagents for cell experiments were purchased from Gibco (USA).
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3

Curcumin Nanoparticles for Alzheimer's

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Curcumin (Cur, ~ 65% pure), Aβ42, 1,1,1,3,3,3-Hexafluoro isopropanol (HFIP) and other accessory chemicals were procured from Sigma (St. Louis, MO). The glial fibrillary acidic protein (GFAP) and Iba-1 (ionized calcium-binding adapter molecule 1) antibodies were purchased from Abcam (Cambridge, MA) and Wako (Richmond, VA), respectively. The Aβ oligomer specific (A11) and fibril specific antibodies (OC) were purchased from Chemicon International (Billerica, Massachusetts), and 6E10 was purchased from BioLegend (San Diego, CA). Solid lipid Curcumin particles (SLCP, which contains 26% Cur) was gifted from the Verdure Sciences (Noblesville, IN). This SLCP consists of high-purity, long-chain phospholipid bilayer and a long-chain fatty acid solid lipid core, which coats the Cur. The SLCP has been well characterized by us [32 (link)] and others in vitro, animal models [30 (link), 33 (link)–35 (link)] and in clinical trials of AD [26 (link)].
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4

Silk-based Biomaterial Fabrication

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kappa-carrageenan, lithium bromide (LiBr), sodium carbonate (Na2CO3), cellulose dialysis tube (12 KDa, MWCO), ninhydrin, 1,1,1,3,3,3-Hexafluoroisopropanol (HFIP), penicillin, chloroform (CHCl3), and [3-(4,-dimethylthiazol-2-y1)-2, 5-diphenyl tetrazolium bromide] were obtained from Sigma-Aldrich (USA), absolute ethanol 99.8%, GP, and isopropanol were provided from Merck Company (Germany). In addition, silk cocoons from Bombyx mori were purchased from the Golestan Silkworm Research Institute (Iran). ALP’s kit was obtained from Pars Azmun (Iran). Furthermore, fetal bovine serum (FBS) and other components of cell culture were purchased from Vasegh Company (Iran).
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5

Amyloid-β Peptide Modification and Conjugation

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Peptides [CHQKLVFFAED and CHQKLVFFAED-YNGK-HQKLVFFAED] were purchased from Peptide 2.0 Inc. (VA, USA). N-hydroxysuccinimide (NHS) and maleimide (MAL) functionalized Cyanine3 dyes (Cyanine3-NHS and Cyanine3-MAL) were purchased from Lumiprobe Corporation (Florida, USA). MAL-PEG-SVA (M.W 3400 g/mol) and mPEG-SVA (M.W 2000 g/mol) were from Laysan Bio (Arab, AL), Tris-(2-Carboxyethyl) phosphine, Hydrochloride (TCEP) was from Hampton Research Inc. (CA, USA), β-mercaptoethanol and 1,1,1,3,3,3 Hexafluoroisopropanol (HFIP) were purchased from Sigma-Aldrich, USA. The synthesis of maleimide-polyethylene glycol-silatrane (MAS) and 1-(3-aminopropyl) silatrane (APS) was described in our previous publication40 (link).
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6

Polycaprolactone Gelatin Antimicrobial Scaffold

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Autoclaved phosphate-buffered saline (1× PBS) was used for all in vitro experiments. PCL (Mn = 80,000), gelatin from bovine skin, DAPI, hydrochloric acid, sodium nitrite, copper(II) chloride, l-ascorbic acid sodium salt, acetone, Cell Counting Kit-8 (CCK-8), and 1,1,1,3,3,3-hexafluoroisopropanol (HFIP) were purchased from Sigma-Aldrich (St. Louis, MO). l-Glutathione (reduced 98+%) was obtained from Alfa Aesar (Ward Hill, MA). Alexa Fluor 488 Phalloidin was purchased from Thermo Fisher Scientific (Waltham, MA). Dulbecco’s modified Eagle’s medium (DMEM) and trypsin–EDTA were purchased from Corning (Manassas, VA). The antibiotic penicillin–streptomycin (Pen–Strep) and fetal bovine serum were purchased from Gibco-Life Technologies (Grand Island NY 14072). The mouse fibroblast cell line (ATCC 1658) and Staphylococcus aureus (ATCC 5538) were originally obtained from American Tissue Culture Collection (ATCC).
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7

Amyloid-β Inhibition by Curcumin and Boronic Acids

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Trans 2-phenyl vinylboronic acid (TPVA) MIDA ester (catalog no: 699292-5G), Aβ42 peptide, curcumin (Cur, ~65% pure), 1,1,1,3,3,3-hexafluoroisopropanol (HFIP), penicillin/streptomycin, fluoro-mount media and other accessory chemicals were procured from Sigma (St. Louis, MO, USA). Trans-beta-styryl-boronic acid (TBSA) was purchased from Alfa Aesar (97% pure, catalog no: H53227, Haverhill, MA). Aβ antibodies, such as 6E10 were purchased from Bio-Legend (San Diego, CA, USA). Mouse neuronal cell line and were purchased from American type culture collection (ATCC, Manassas, VA, USA). DePeX mounting media was purchased from BDH (Radnor, PA, USA). Minimum essential medium (MEM), fetal bovine serum (FBS) were purchased from GIBCO (Grand Island, NY, USA). Ninety-six-well plate was purchased from COSTAR Corning, (Millipore-Sigma, St. Louis, MO, USA). Horseradish peroxidase-conjugated secondary antibody was purchased from Santa Cruz Biotech (Santacruz, CA, USA). The GFAP antibody was procured from Cell Signaling Technology (Danvers, MA, USA) and Iba-1 was purchased from FUJIFILM Wako Chemicals U.S.A. Corporation (Richmond, VA, USA).
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8

Preparation of Graphene Oxide-Embedded PLGA Scaffolds

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PLGA (molecular weight = 80000, LA/GA = 75/25) was purchased from SinoBiomaterials Co., Ltd. BMP-2 was purchased from UB Biotech. HA powder was obtained from Nanjing Emperor Nano Material Co., Ltd. GO was purchased from Chengdu Organic Chemicals Co. Ltd, China (thickness: 0.55–1.2 nm diameter: 0.5–3 μm). Bovine serum albumin (BSA) was obtained from Beijing Solarbio Science & Technology Co., Ltd. Dichloromethane (DCM) was obtained from Beijing Chemical Works. 1,1,1,3,3,3-hexafluoroisopropanol (HFIP), 3-(4,5-dimethyl-2-thiazolyl)-2, 5-diphenyl-2- H-tetrazolium bromide (MTT) and BCA protein assay kit were purchased from Sigma-Aldrich (USA). Cetylpyridinium chloride (CPC) was purchased from Aladdin Chemistry Co. Ltd. The reagents for cell experiments were purchased from Gibco (USA).
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9

Silk Cocoon-Based Biomaterials Characterization

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Bombyx mori (B. mori) silk cocoons were obtained from Paradise Fibers (USA). ZN from maize (Z3625), DAPI, hydrochloric acid, sodium nitrite, methanol (≥99.8%), lithium bromide, Cell Counting Kit-8 (CCK-8), and 1,1,1,3,3,3-hexafluoroisopropanol (HFIP) were purchased from Sigma-Aldrich (St. Louis, MO). Trypsin (1:250) for the degradation test was purchased from GoldBio. Acetone was purchased from VWR (Radnor, PA). l-glutathione (reduced 98+%) was obtained from Alfa Aesar (Ward Hill, MA). Spectra/Por 3 regenerated cellulose dialysis membrane tubings (3.5 kDa molecular weight cutoff) and Alexa Fluor 488 Phalloidin were purchased from Thermo Fisher Scientific (Waltham, MA). Dulbecco’s modified Eagle’s medium (DMEM) and Trypsin–EDTA were purchased from Corning (Manassas, VA). The penicillin–streptomycin (Pen–Strep) antibiotic and fetal bovine serum (FBS) were bought from Gibco-Life Technologies (Grand Island NY 14072). LB broth was obtained from Fisher Bioreagents. LB Agar was obtained from Difco Laboratories. The bacterial strains Staphylococcus aureus (ATCC 6538) and Escherichia coli (ATCC 25922) and 3T3 mouse fibroblast cells (ATCC 1658) were purchased from American Type Culture Collection (ATCC).
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10

Fabrication of Biomimetic Microfluidic Devices

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Silicon wafer with crystal phase of 100 was purchased from RDMICRO (Suzhou, China). Concentrated sulfuric acid, hydrogen peroxide, glutaraldehyde and PCL (80 000 Mw) were obtained from Chinese Medicine Group. SU-8 GM 1070 photoresist were from Gersteltec Sarl, CH. Polydimethylsiloxane (PDMS) was from Dow Corning Corp, USA. Diacetone alcohol and Propylene Glycol Monomethyl Ether Acetate (PGMEA) were purchased from Micro-Chem, USA. 1,1,1,3,3,3-Hexafluoroisopropanol (HFIP) was purchased from Sigma-Aldrich, USA. RPMI Medium 1640 basic (Gibco, USA), low glucose DMEM Medium (Gibco, USA), fetal bovine serum (HyClone, USA), penicillin–streptomycin solution (HyClone, USA), trypsin (HyClone, USA) and PBS (HyClone, USA) were used for cell culture. NCTC clone 929 (L-929) and bone mesenchymal stem cells (MSCs) were purchased from Cyagen.
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