We used the following primary antibodies: mouse monoclonal Bassoon (1:400, immunocytochemistry [ICC]; 1:1,000, Western blot [WB]; ADI-VAM-PS003; Enzo Life Sciences), rabbit monoclonal K48 polyUb (Apu2; 1:500, ICC; 1:1,000, WB; 05-1307; EMD Millipore), chicken polyclonal MAP2 (1:5,000, ICC; AB5543; EMD Millipore), chicken polyclonal tau (1:1,000, ICC; ab75714; Abcam), mouse monoclonal tubulin (1:300,000, WB; T7816; Sigma-Aldrich), mouse monoclonal tuj1 (1:1,000, ICC; MMS-435P; Covance), rabbit polyclonal Ub (1:200, ICC; 1:1,000, WB; Z0458; Dako), guinea pig polyclonal VGluT1 (1:1,500, ICC; AB5905; EMD Millipore), and rabbit polyclonal VGluT1 (1:5,000, WB; 135503; Synaptic Systems). As for secondary antibodies, alkaline phosphatase–conjugated antibodies (Jackson ImmunoResearch Laboratories, Inc.) were used for blotting, and Alexa Fluor 350–, 488–, 568–, and 647–conjugated antibodies (1:1,000; Thermo Fisher Scientific) were used for immunocytochemistry.
Alkaline phosphatase conjugated antibodies
Manufactured by Jackson ImmunoResearch
Sourced in United States
Alkaline phosphatase–conjugated antibodies are laboratory reagents used for various immunochemical techniques. They consist of antibodies that have been chemically linked to the enzyme alkaline phosphatase. This enzyme can be detected through colorimetric or chemiluminescent reactions, allowing for the visualization and quantification of target analytes in samples.
Automatically generated - may contain errors
Lab products found in correlation
Ab75714, by Abcam (1 mentions)
Z0458, by Agilent Technologies (1 mentions)
Adi vam ps003, by Enzo Life Sciences (1 mentions)
Ab5543, by Merck Group (1 mentions)
T7816, by Merck Group (1 mentions)
Ab5905, by Merck Group (1 mentions)
Mms 435p, by Fortrea (1 mentions)
Polyvinylidene difluoride membrane, by Bio-Rad (1 mentions)
2 protocols using alkaline phosphatase conjugated antibodies
1
Antibody Labeling Protocols for Neurodegenerative Research
2
Western Blot Analysis of CgA and CgB
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Samples from whole brain and whole adrenal glands from P1 mice were separated by sodium dodecyl sulfate–polyacrylamide gel electrophoresis in 8% acrylamide gels and electroblotted onto 0.22 μm polyvinylidene difluoride membranes (Bio‐Rad Laboratories, Veenendaal, The Netherlands). Membranes were probed with the following primary antibodies: rabbit polyclonal anti‐CgA (Cat# 259 003, RRID: AB_2619972; Synaptic Systems, Goettingen, Germany), rabbit polyclonal anti‐CgB (Cat# 259 103, RRID: AB_2619973; Synaptic Systems), and mouse monoclonal anti‐actin (Cat# MAB1501, RRID: AB_2223041; Millipore Corporation, Bedford, MA, USA). Alkaline phosphatase‐conjugated antibodies (Jackson Immuno‐Research, West Grove, PA, USA) were used to visualize the protein bands using the AttoPhos® AP Fluoresent Substrate System (Promega, Madison, WI, USA). Membranes were scanned with a FLA‐5000 Fujifilm device and analyzed with the software ImageJ (NIH, Bethesda, Maryland, USA).
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