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Dryslide nitrocefin

Manufactured by BD
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Sourced in Canada

The DrySlide Nitrocefin is a laboratory equipment product. It is a chromogenic cephalosporin that is used to detect the presence of beta-lactamase enzymes in bacterial samples.

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Lab products found in correlation

Antibiotic disk, by Thermo Fisher Scientific (1 mentions) Etest, by bioMérieux (1 mentions)

2 protocols using dryslide nitrocefin

1

Antibiotic Susceptibility Testing Protocol

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Antibiotic susceptibility disk diffusion testing was conducted according to CLSI guidelines (20 ) using the following antibiotic disks from Oxoid (Nepean, Ontario, Canada): ampicillin 10 µg; chloramphenicol 30 µg; ceftriaxone 30 µg; trimethoprim–sulfamethoxazole 25 µg; amoxicillin–clavulanic acid 30 µg; cefaclor 30 µg; ciprofloxacin 5 µg; moxifloxacin 5 µg; clarithromycin 15 µg; azithromycin 15 µg; tetracycline 30 µg; levofloxacin 5 µg and meropenem 10 µg. Production of β-lactamase was determined using DrySlide Nitrocefin (Becton Dickinson).
Tsang R.S., Li Y.A., Mullen A., Baikie M., Whyte K., Shuel M., Tyrrell G., Rotondo J.A., Desai S, & Spika J. (2016). Laboratory characterization of invasive Haemophilus influenzae isolates from Nunavut, Canada, 2000–2012. International Journal of Circumpolar Health, 75, 10.3402/ijch.v75.29798.
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2

Antibiotic Susceptibility Testing Protocol

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β-Lactamase production was determined using DrySlide Nitrocefin (BBL, Becton Dickinson, Oakville, ON, Canada). To determine the MICs of penicillin, ceftriaxone, chloramphenicol, rifampicin and ciprofloxacin, Etest (AB Biodisk, Solna, Sweden) was performed on Mueller–Hinton agar with 5% blood according to CLSI guidelines.19 Penicillin MICs were also determined by the agar dilution method.23 (link)
Tsang R.S., Ahmad T., Jamieson F.B, & Tyrrell G.J. (2018). WGS analysis of a penicillin-resistant Neisseria meningitidis strain containing a chromosomal ROB-1 β-lactamase gene. Journal of Antimicrobial Chemotherapy, 74(1), 22-28.
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