Brca2

Manufactured by Proteintech

BRCA2 is a recombinant protein produced in-house by Proteintech's experts. It is a DNA repair protein that plays a crucial role in homologous recombination, a process that helps maintain genomic stability.

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Lab products found in correlation

Lin37, by Santa Cruz Biotechnology (1 mentions) Mre11, by Novus Biologicals (1 mentions) Gapdh, by Merck Group (1 mentions) Phospho rb ser807 811, by Cell Signaling Technology (1 mentions) Brca1, by R&D Systems (1 mentions) Phospho rb ser780, by Cell Signaling Technology (1 mentions) Cyclin b1, by Abcam (1 mentions) Cyclin d1, by ABclonal (1 mentions) Ripa lysis buffer, by Beyotime (1 mentions) β actin, by ZenBio (1 mentions) Protease inhibitor cocktail, by Thermo Fisher Scientific (1 mentions) H3k9me2, by ABclonal (1 mentions) H3k9me3, by ABclonal (1 mentions) 3 3 diaminobenzidine (dab), by Agilent Technologies (1 mentions) Proliferating cell nuclear antigen (pcna), by Abcam (1 mentions) Rad18, by Abcam (1 mentions) Brca1, by Biocare Medical (1 mentions) Rad51, by Abcam (1 mentions)

3 protocols using brca2

Antibody Panel for DNA Damage Signaling

Cited 1 time

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The following antibodies were used for western blot analysis: 53BP1 (Bethyl Laboratories, A300-272A, 1:3000), LIN37 (Santa Cruz Biotechnology, sc-515686, 1:200), BLM (Bethyl Laboratories, A300-572A, 1:2000), BRCA1 for mouse (R and D Systems, gift from Dr. Andre Nussenzweig, NCI, 1:1000) (Zong et al., 2019 (link)), BRCA1 for human (Millipore Sigma, 07-434, 1:1000), RAD51 (Millipore Sigma, ABE257, 1:2000), BARD1 (Thermo Fisher Scientific, PA5-85707, 1:1000), CtIP (gift from Dr. Richard Baer, [Columbia University, New York], 1:1000), MRE11 (Novus Biologicals, NB100-142, 1:2000), RIF1 (Abcam, ab13422, 1:500), SHLD1/C20orf196 (Thermo Fisher Scientific, PA5-559280, 1:200), GAPDH (Sigma, G8795, 1:10,000), KAP1 (Genetex, GTX102226, 1:2000), FANCD2 (R and D Systems, MAB93691, 1:1000), BRCA2 for human (Proteintech, 19791-1-AP, 1:500), Rb1 (Thermo Fisher Scientific, LF-MA0173, 1:1000), Phospho-Rb (Ser780) (Cell Signaling Technology, 8180T, 1:1000), Phospho-Rb (Ser807/811) (Cell Signaling Technology, 8516T, 1:1000), PCNA (Bethyl Laboratories, A300-276A, 1:3000), CDK4: (Novus Biologicals, NBP1-31308, 1:1000), CDK4 (phosphor Thr 172) (GeneTex, GTX00778, 1:1000), and RPA (Cell Signaling Technology, 2208S, 1:1000).

Chen B.R., Wang Y., Tubbs A., Zong D., Fowler F.C., Zolnerowich N., Wu W., Bennett A., Chen C.C., Feng W., Nussenzweig A., Tyler J.K, & Sleckman B.P. (2021). LIN37-DREAM prevents DNA end resection and homologous recombination at DNA double-strand breaks in quiescent cells. eLife, 10, e68466.

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Protein Extraction and Western Blot Analysis

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Protein extraction was conducted using RIPA Lysis Buffer (Beyotime Biotechnology, Shanghai, China) containing a protease inhibitor cocktail (Thermo Fisher Scientific, USA). Sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) assay was performed as in previous reports.^{52 (link)} Primary antibodies used in this study were β-actin (cat. 200068-8F10; ZEN-bioscience), H3 (cat. ASO71; ABclonal), Cyclin D1 (cat. 55506 T; CST), CDK4 (cat. 12790 T; CST), Cyclin A2 (cat. ab181591; Abcam), Cyclin E1 (cat. 20808 S; CST), CDK1 (cat. ab32094; Abcam), Cyclin B1(cat. 55004-1-AP; Proteintech), H3K9me1 (cat. A2358; ABclonal), H3K9me2 (cat. A2359; ABclonal), H3K9me3 (cat. ab176916; Abcam), MDIG (cat. 12214-1-AP; Proteintech), OTX2 (cat. 13497-1-AP; Proteintech), Myc (cat. 10828-1-AP; Proteintech), BRCA2 (cat. AF7817; Affinity), Junb (cat. 10486-1AP; Proteintech), E2F2 (cat. AF4100), ATAD5 (cat. Bs-7656R; Bioss). Western blots were quantified using ImageJ software (Version 1.51n, National Institutes of Health, Bethesda, MD, https://imagej.net/ij/index.html), as previously described.^{53 (link)} The original and uncropped films of Western blots were provided as Supplementary Figs. S19–S21.

Du J., Liao W., Wang H., Hou G., Liao M., Xu L., Huang J., Yuan K., Chen X, & Zeng Y. (2023). MDIG-mediated H3K9me3 demethylation upregulates Myc by activating OTX2 and facilitates liver regeneration. Signal Transduction and Targeted Therapy, 8, 351.

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Immunohistochemical Analysis of DNA Repair Proteins

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Slides were baked at 60°C for one hour. After deparaffinization and rehydration, tissue sections were treated with either citrate buffer or Borg Decloaker for five minutes in a pressure cooker for antigen retrieval. Hydrogen peroxide (3%) was applied to the sections for 10 minutes. Sections were incubated with primary antibodies against BRCA1 (Biocare Medical), BRCA2 (Proteintech), PCNA, RAD51 (Abcam), RAD6, RAD18 or RPA1/RPA70 (Abcam) for 30 minutes. After buffer rinsing, sections were incubated with anti-mouse HRP-labeled polymer (EnVision) or anti-HRP-labeled polymer (Mach2) for 30 minutes and buffer rinsed twice. Finally, the staining was visualized by DAB+ (Dako). IHC staining was performed using the IntelliPATH FLX Automated Stainer at room temperature.
A light hematoxylin counterstain was performed, then slides were dehydrated, cleared, and mounted using permanent mounting media.

Holcomb A.J., Brown L., Tawfik O., Madan R., Shnayder Y., Thomas S.M, & Wallace N.A. (2020). DNA Repair Gene Expression is Increased in HPV Positive Head and Neck Squamous Cell Carcinomas.. Virology, 548, 174-181.

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