Animal experiments were approved by UCSF Institutional Animal Care and Use Committee. Animals were housed in communal cages in a temperature- and humidity-controlled environment with 12 hour light/dark cycle and provided standard rodent chow and water ad libitum. Wild-type female CD1 mice were bred in the UCSF Laboratory Animal Resource Center. Female C3H/HeJ mice, which have a spontaneous mutation in TLR4 (Tlr4lps-d) and their control background C3H/HeOuJ mice were purchased from Jackson Laboratories (Bar Harbor, Maine).
Female c3h hej mice
Manufactured by Jackson ImmunoResearch
Sourced in United States
Female C3H/HeJ mice are a laboratory mouse strain commonly used in biomedical research. These mice are genetically modified and have a specific genetic background that makes them suitable for various experimental applications.
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10 protocols using female c3h hej mice
1
Mouse Housing and Breeding Protocol
2
C3H/HeJ Mice in Pathogen-Free Facility
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Female C3H/HeJ mice 4–6 weeks of age were purchased from The Jackson Laboratory (Bar Harbor, ME, USA). C3H TLR2−/− mice at the N6 generation backcross were generously provided by Dr. Linda Bockenstedt (Yale University) and these were fully backcrossed onto the C3H/HeJ background (N10) in our colony. Animals were given sterile food and water ad libitum and housed in a specific pathogen-free facility. All works were done in accordance with the Animal Care and Use Committee of the University of Missouri.
3
C3H/HeJ Mice Procurement Protocol
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Female C3H/HeJ mice were purchased from The Jackson Laboratory, Bar Harbor, ME, USA.
4
Murine Experimental Protocols for UBC
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Female C3H/HeJ mice aged nine weeks were purchased from The Jackson Laboratory (Bar Harbor, Marine). The University of British Columbia Animal Care Committee approved all experimental procedures. The methods were carried out in accordance with the approved guidelines. Mice were socially housed under a 12 hour light/dark cycle.
5
Induction of Alopecia Areata in Female C3H/HeJ Mice
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Female C3H/HeJ mice were obtained from The Jackson Laboratory and were maintained under pathogen-free conditions and a 12-h light/dark cycle at an ambient temperature of 22 to 26 °C. Female mice were used because like numerous other autoimmune disorders (93 (link)), AA has a slight female predominance (94 (link), 95 (link)). This female predominance is observed in the skin-graft C3H/HeJ mouse model of AA (96 (link)), in which the literature reported that 95 to 100% of grafted Female C3H/HeJ mice developed AA, whereas the incidence was 30% in male C3H/HeJ mice (23 (link), 96 (link)). To induce AA in C3H/HeJ mice, we grafted full-thickness skin (approximately 1 cm in diameter) from donor mice with established disease onto 8- to 10-wk-old naive syngeneic recipients, as previously described (3 (link)). Animals were housed and all experiments were performed in compliance with institutional guidelines as approved by the Institutional Animal Care and Use Committee at Columbia University.
6
Female C3H/HeJ Mice Experiments
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Female C3H/HeJ mice (The Jackson Laboratory) were used in these experiments. Animals were 8–12 weeks of age and maintained/bred in The La Jolla Institute for Immunology vivarium under specific-pathogen-free conditions in accordance with guidelines of the Association for Assessment and Accreditation of Laboratory Animal Care International and animal studies were approved by The La Jolla Institute for Immunology Institutional Animal Care and Use Committee.
7
Standardized Housing for C3H/HeJ Mice
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Female C3H/HeJ mice (Jackson Laboratories, USA), 5 weeks old at arrival, were randomly allocated to groups (n = 11–13) and housed on Nestpack bedding (Datesand Ltd, Manchester, UK), 3–5 mice in each cage. The Harlan Teklad 2018 rodent diet and tap water were given ad libitum. The mice were exposed to a 12/12 h light/dark cycle, room temperature (RT) of 21 +/− 2 °C and 55 +/− 10 % humidity. The experiment was performed in conformity with the laws and regulations for live animals in Norway, and approved by the Norwegian Animal Research Authority under the Ministry of Agriculture (FOTS ID 4534).
8
Isolation and Differentiation of Bone Marrow-Derived Macrophages
9
Acclimation of C3H/HeJ Mice
10
Mouse Housing and Acclimation Protocol
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C3H/HeJ female mice, aged 6‐8 weeks, were purchased from Jackson laboratories. Before performing any experiment, all mice were housed in specific pathogen‐free conditions for at least 1 week for acclimatization at the AAALAC‐accredited animal facility at the University of South Carolina, School of Medicine (Columbia, SC). All experimental procedures performed were approved by the University of South Carolina Institutional Care and Use Committee (IACUC) according to the guidelines set by the Care and Use of Laboratory Animals of the National Research Council under AUP# 2169.
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