The TKCP solution was added to different concentrations of KO2 (0 μM, 50 μM and 100 μM) with or without ROS inhibitor (N-acetyl-l -cysteine, NAC) and then incubated at 37 °C for 0, 1, 2, 4, 8 and 24 h, respectively. The fluorescence signals were captured by using In-vivo Multispectral Imaging Systems (Bruker, Germany).
In vivo multispectral imaging system
Manufactured by Bruker
Sourced in Germany
The In-Vivo Multispectral Imaging System is a lab equipment product developed by Bruker. It is designed to capture multispectral images of small animals or tissues. The system uses multiple wavelengths of light to generate detailed images that can provide information about various biological processes or characteristics within the sample.
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Lab products found in correlation
6 protocols using in vivo multispectral imaging system
1
Quantifying KO2-Induced Oxidative Stress
2
In Vivo Bioimaging of Osteoarthritis Treatments
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For in vivo bioimaging, mice were anesthetized by isoflurane. Each group of OA mice (n = 3) was IA injected with 50 μL of 400 μg/mL TKCP, CAPP or TKP, and the normal mice (n = 3) were also IA injected with TKCP as control. The images were captured by an In-vivo Multispectral Imaging Systems (Bruker, Germany) at 0, 1, 2, 4, 7 and 14 days.
In addition, the fluorescence intensity of the TKCP was also investigated. The mice were randomly sorted into three groups (n = 3): 0.05 mg MIA + inhibitor group, IA injection of 0.05 mg MIA concomitant with 5 mM NAC; 0.05 mg MIA group, IA injection of 0.05 mg MIA; 0.1 mg MIA group, IA injection of 0.1 mg MIA [44 (link), 45 (link)]. Finally, the mice were sacrificed and the macroscopic evaluations of joints were performed.
In addition, the fluorescence intensity of the TKCP was also investigated. The mice were randomly sorted into three groups (n = 3): 0.05 mg MIA + inhibitor group, IA injection of 0.05 mg MIA concomitant with 5 mM NAC; 0.05 mg MIA group, IA injection of 0.05 mg MIA; 0.1 mg MIA group, IA injection of 0.1 mg MIA [44 (link), 45 (link)]. Finally, the mice were sacrificed and the macroscopic evaluations of joints were performed.
3
In Vivo Cathepsin K Imaging in Osteoclasts
4
Radioactive Copper-64 Labeling of αSTn and αSTn-IgG4 Targeting Molecules
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All solvents were purchased from commercial sources (Sigma−Aldrich, Fluka, VWR, Fisher Scientific). The no carrier added (NCA) [64Cu]Cu2+ was produced at the Helmholtz-Zentrum Dresden-Rossendorf on a TR-Flex (Advanced Cyclotron Systems Inc.) by 64Ni(p,n)64Cu nuclear reaction and prepared as reported previously [27 (link)]. For labeling of the αSTn and αSTn-IgG4 TMs with 64Cu, [64Cu]CuCl2 (200 MBq in 0.01 M HCl, 0.3 M NH4OAc, pH 5.0) was added to 9.2 ± 6.7 nmol of the TMs and tempered at 38 °C for 30 min. Labeling yield and radiochemical purity were determined using radio thin-layer chromatography (radio-ITLC). The radio-ITLC was carried out on SG stripes (Merck) using 0.1 M citrate, 0.01 M EDTA. The developed chromatograms were analyzed by autoradiography using the In-vivo Multispectral Imaging System (Bruker).
5
Fluorescence Imaging of Tumor Targeting
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The TIP-1 mAb 2C6F3 or the normal mouse IgG (NM-IgG) was labeled with Alexa Fluor 750 as per manufacturer's instructions (Thermo Fisher). Tumors were induced by injecting LLC (0.5 × 106) or GL261 (1 × 106) cells in both the hind limbs of nude mice. The right hind limb tumors were irradiated with 3 fractions of 3 Gy over a course of 24 h while the tumors on the left hind limb were used as the sham-irradiated controls. The tumor-bearing mice were then injected with 50 μg of Alexa Flour-750 labeled 2C6F3 (2C6F3-AF750) or normal mouse IgG (IgG-AF750) via the tail vein. For optical imaging, the mice were anesthetized with 2% isoflurane and imaged using the In-Vivo Multispectral Imaging System (Bruker Biospin). Fluorescence was detected using 730 nm excitation and 790 nm emission filters with 60 s acquisition time, F-stop 2.4, and 2 × 2 binning. ROI analysis was performed using NIH ImageJ image processing software and mean fluorescence intensity values reported as arbitrary units (a.u.).
6
In Vivo Fluorescence Imaging of Nanodrug Retention
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To analyze the nanodrug retention time in vivo, we used an in vivo Multispectral Imaging System (Bruker, Germany) to detect the fluorescence signals of PCFMN and PFMN labeled with DID in the knee OA model. After IA injection, fluorescence imaging was conducted (n = 3). Images were obtained at corresponding time points (0, 1, 3,7, 19 d).
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