Total RNAs were extracted from paired HCC and non-cancerous tissues or cultured cells using Trizol reagent (Invitrogen, Carlsbad, CA, USA) following the manufacturer’s protocol. RT and qPCR kits (Takara, Dalian, China) were used to determine the expression of CCAT2 in tissue samples and cultured cells. qRT-PCR was done using SYBR1 Premix Ex Taq II (Tli RNaseH Plus) (Takara) on ABI 7900HT (Applied Biosystems, Foster City, CA, USA). The primers used in this study are shown in Table 1 . The relative expression of CCAT2 was normalized to reference glyceraldehyde-3-phosphate dehydrogenase (GAPDH) and was calculated.
Rt and qpcr kits
Manufactured by Takara Bio
Sourced in China
Takara Bio's RT (Reverse Transcription) and qPCR (Quantitative Polymerase Chain Reaction) kits are designed for the detection and quantification of RNA molecules. These kits provide the necessary reagents and protocols for the efficient conversion of RNA into cDNA (complementary DNA) and the subsequent amplification and real-time monitoring of the target sequences.
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Lab products found in correlation
3 protocols using rt and qpcr kits
1
Quantifying CCAT2 Expression in HCC
2
Quantification of LINC00261 Expression
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Total RNAs were extracted from tumorous and adjacent normal tissues or cultured cells using Trizol reagent (Invitrogen) following the manufacturer’s protocol. RT and qPCR kits (Takara, Dalian, China) were used to evaluate the expression of LINC00261 in tissue samples and cultured cells. The primers used in this study are shown in Additional file 4: Table S1 . Real-time PCR was performed in triplicate, and the relative expression of LINC00261 was calculated using the comparative cycle threshold (2−ΔΔCT) method with glyceraldehyde-3-phosphate dehydrogenase (GAPDH) as the endogenous control to normalize the data.
3
Quantification of FENDRR Expression
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Total RNAs were extracted from tumorous and adjacent normal tissues or cultured cells using Trizol reagent (Invitrogen) following the manufacturer’s protocol. RT and qPCR kits (Takara, Dalian, China) were used to evaluate the expression of FENDRR in tissue samples and cultured cells. The primers used in this study are shown in Additional file 5 : Table S1. Real-time PCR was performed in triplicate, and the relative expression of FENDRR was calculated using the comparative cycle threshold (CT) (2−ΔΔCT) method with glyceraldehyde-3-phosphate dehydrogenase (GAPDH) as the endogenous control to normalize the data.
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