300b lr

Manufactured by Aurora Scientific

Sourced in Canada

The 300B-LR is a precision scientific instrument designed for laboratory applications. It features a stable and reliable power supply with regulated voltage and current output. The 300B-LR is a compact and versatile device suitable for a range of experimental setups and research environments.

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Lab products found in correlation

Model 300c lr, by Aurora Scientific (1 mentions) Model e2 12, by Natus (1 mentions)

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Model 300B-LR

5 protocols using 300b lr

Measuring Maximal Contractile Properties of Murine Extensor Digitorum Longus Muscle

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Mice were anesthetized with sodium pentobarbital (75 mg/kg body mass). EDL muscles (15.10 ± 0.12 mg; 13.36 ± 0.04 mm; n = 208) were removed and mounted on a dual-mode muscle lever system (300B-LR; Aurora Scientific Inc.) with 5–0 suture in a 1.2 mL bath assembly filled with oxygenated (95:5% O₂:CO₂) Krebs-Ringer bicarbonate buffer maintained at 25 °C. Muscles were adjusted to their anatomical optimal length (L_o) based on resting tension [35 (link)]. Muscles remained quiescent in the bath for 5 min before performing maximal isometric tetanic contractions every 2 min. The muscle was stimulated to contract using maximal voltage (150 V) for 200 ms at 175 Hz until force plateaued within 5 mN from one contraction to the next (381 ± 4 mN; 15.80 ± 0.16 N/cm²). Maximal rate of tetanic contraction (+ dP/dt) and relaxation (− dP/dt) were calculated from maximal isometric tetanic force (P_o) and muscle length was measured from myotendinous junction to myotendinous junction using digital calipers.

Lindsay A., Baumann C.W., Rebbeck R.T., Yuen S.L., Southern W.M., Hodges J.S., Cornea R.L., Thomas D.D., Ervasti J.M, & Lowe D.A. (2020). Mechanical factors tune the sensitivity of mdx muscle to eccentric strength loss and its protection by antioxidant and calcium modulators. Skeletal Muscle, 10, 3.

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Isolation and Measurement of Soleus Muscle

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Soleus muscle isolation and preparation have previously been described (Moran et al., 2005 (link)). Briefly, the soleus muscle was placed in a 1.2 mL bath filled with oxygenated Krebs-Ringer buffer maintained at 25°C. The proximal muscle tendon was attached by 6–0 suture to a dual-mode muscle lever system (300B-LR; Aurora Scientific Inc.). Measures of peak isometric, concentric, and eccentric forces during tetanic stimulation were obtained as previously described (Greising et al., 2011b (link)).

Cabelka C.A., Baumann C.W., Lindsay A., Norton A., Blixt N.C., Le G., Warren G.L., Mansky K.C., Novotny S.A, & Lowe D.A. (2021). Tissue selective effects of bazedoxifene on the musculoskeletal system in female mice. The Journal of endocrinology, 248(2), 181-191.

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Isometric Ankle Dorsiflexion Torque Measurement

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In vivo peak isometric torque of the ankle dorsiflexors was assessed as previously described (Baltgalvis, Call, Nikas, & Lowe, 2009 (link)). Briefly, anaesthesia was induced using an induction chamber and 5% isoflurane in oxygen. Anaesthesia was maintained using 1.5% isoflurane at an oxygen flow rate of 0.4 l min⁻¹. The left hindlimb was depilated and aseptically prepared and the foot placed in a foot-plate attached to a servomotor (Model 300C-LR; Aurora Scientific, Aurora, Ontario, Canada). Platinum–iridium needles (Model E2–12; Grass Technologies, West Warwick, RI, USA) were placed on either side of the left common peroneal nerve to elicit contraction of the dorsi-flexors muscles. Peak isometric torque was defined as the greatest torque measured by a 300B-LR servomotor (Aurora Scientific) during a 200 ms stimulation using 1 ms square-wave pulses at 300 Hz and increasing voltage from 3.0 to 9.0 V (models S48 and SIU5; Grass Technologies). All torque values were normalized by mouse body mass.

Southern W.M., Nichenko A.S., Qualls A.E., Portman K., Gidon A., Beedle A.M, & Call J.A. (2020). Mitochondrial dysfunction in skeletal muscle of fukutin-deficient mice is resistant to exercise- and 5-aminoimidazole-4-carboxamide ribonucleotide-induced rescue. Experimental physiology, 105(10), 1767-1777.

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Measuring Muscle Contractile Properties

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Extensor digitorum longus (EDL) and soleus (SOL) muscles were excised and analyzed for force-generating capacities in vivo, as previously described [51 (link),52 (link),53 (link)]. Muscles were mounted on a dual-mode muscle lever system (300B-LR; Aurora Scientific Inc., Aurora, ON, Canada) in a 0.38-mL bath filled with Krebs Ringer bicarbonate that was maintained at 25 °C. Contractile characteristics tested included the following: peak twitch force, peak isometric tetanic force, time-to-peak twitch force and twitch half-relaxation time, and the maximal rates of tetanic contraction and relaxation.

Nichenko A.S., Sorensen J.R., Southern W.M., Qualls A.E., Schifino A.G., McFaline-Figueroa J., Blum J.E., Tehrani K.F., Yin H., Mortensen L.J., Thalacker-Mercer A.E., Greising S.M, & Call J.A. (2021). Lifelong Ulk1-Mediated Autophagy Deficiency in Muscle Induces Mitochondrial Dysfunction and Contractile Weakness. International Journal of Molecular Sciences, 22(4), 1937.

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Measuring Mouse Muscle Contractility

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Intact EDL muscles were dissected from anesthetized mice and placed in a tissue bath filed with Krebs-Ringer bicarbonate buffer. The distal tendon was statically attached to the bottom of the bath and the proximal tendon to a lever. Contractions were induced using a Grass S48 stimulator delivered through a SIU5D stimulus isolation unit (Grass Telefactor), and forces measured using a dual-mode muscle lever system (300B-LR; Aurora Scientific Inc.) and TestPoint software (Capital Equipment), as described in ref. ^{34 (link)}.

Bosnakovski D., Chan S.S., Recht O.O., Hartweck L.M., Gustafson C.J., Athman L.L., Lowe D.A, & Kyba M. (2017). Muscle pathology from stochastic low level DUX4 expression in an FSHD mouse model. Nature Communications, 8, 550.

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