Dylight 680 800

Manufactured by Thermo Fisher Scientific

Sourced in Canada

DyLight 680/800 is a fluorescent dye designed for use in various biological applications, such as labeling proteins, nucleic acids, and other molecules. The dye is available in two different wavelengths, 680 nm and 800 nm, which can be detected using appropriate instrumentation.

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Lab products found in correlation

Odyssey clx imaging system, by Thermo Fisher Scientific (1 mentions) Ipfl00010, by Merck Group (1 mentions) Ab2907, by Merck Group (1 mentions) Anti gapdh, by Santa Cruz Biotechnology (1 mentions) Odyssey scanning system, by LI COR (1 mentions) Anti tubulin, by Merck Group (1 mentions) Odyssey blocking buffer, by LI COR (1 mentions) Halt protease and phosphatase inhibitor cocktail, by Thermo Fisher Scientific (1 mentions)

Spelling variants of this product

DyLight 800 (57 citations) DyLight 680 (42 citations) DyLight 680/800 conjugated secondary antibodies (3 citations) DyLight 800/680 secondary antibodies (2 citations)

2 protocols using dylight 680 800

Co-immunoprecipitation protocol for protein analysis

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At 3 days postinfection (at MOI 0.1), cells were trypsinised and rinsed in ice cold PBS before being lysed in ice-cold lysis buffer [43 (link)]. IPs were set up overnight at 4°C with the antibody and protein lysate in a ratio of 3 μg of antibody per mg of protein lysate. The immunoprecipitated proteins were subjected to SDS-PAGE and then transferred to PVDF membranes (Merck Millipore, IPFL00010), which were then incubated with their respective biotinylated primary antibodies (0.5 to 1.0 mg/ml). Bands were visualised (LI-COR Odyssey CLx imaging system) using labelled streptavidin (DyLight 680/800, Thermo Fisher Scientific, 21848/21851).

Rihn S.J., Merits A., Bakshi S., Turnbull M.L., Wickenhagen A., Alexander A.J., Baillie C., Brennan B., Brown F., Brunker K., Bryden S.R., Burness K.A., Carmichael S., Cole S.J., Cowton V.M., Davies P., Davis C., De Lorenzo G., Donald C.L., Dorward M., Dunlop J.I., Elliott M., Fares M., da Silva Filipe A., Freitas J.R., Furnon W., Gestuveo R.J., Geyer A., Giesel D., Goldfarb D.M., Goodman N., Gunson R., Hastie C.J., Herder V., Hughes J., Johnson C., Johnson N., Kohl A., Kerr K., Leech H., Lello L.S., Li K., Lieber G., Liu X., Lingala R., Loney C., Mair D., McElwee M.J., McFarlane S., Nichols J., Nomikou K., Orr A., Orton R.J., Palmarini M., Parr Y.A., Pinto R.M., Raggett S., Reid E., Robertson D.L., Royle J., Cameron-Ruiz N., Shepherd J.G., Smollett K., Stewart D.G., Stewart M., Sugrue E., Szemiel A.M., Taggart A., Thomson E.C., Tong L., Torrie L.S., Toth R., Varjak M., Wang S., Wilkinson S.G., Wyatt P.G., Zusinaite E., Alessi D.R., Patel A.H., Zaid A., Wilson S.J, & Mahalingam S. (2021). A plasmid DNA-launched SARS-CoV-2 reverse genetics system and coronavirus toolkit for COVID-19 research. PLoS Biology, 19(2), e3001091.

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Quantifying CRT Expression by Western Blot

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Western blotting was employed to determine the total expression of CRT. Briefly, cells were lysed in radioimmunoprecipitation assay buffer with Halt protease and phosphatase inhibitor cocktail (Thermo Fisher Scientific) augmented with 1 mM PMSF. The prepared lysates were separated (25 μg) on 10% SDS-PAGE and transferred to nitrocellulose membranes. The membranes were blocked (Odyssey blocking buffer-Licor, Lincoln) and incubated with anti-CRT primary antibody (Abcam Ab2907 and Ab92516), anti-tubulin (Sigma, Canada), and anti-GAPDH (Santa Cruz Biotechnology Inc) primary antibodies overnight at 4 °C, and with IR-conjugated secondary antibody, DyLight 680/800 (Thermo Fisher Scientific) for 1 h. The stained membranes were scanned using the Odyssey scanning system (Licor).

Bharadwaj A.G., Okura G.C., Woods J.W., Allen E.A., Miller V.A., Kempster E., Hancock M.A., Gujar S., Slibinskas R, & Waisman D.M. (2023). Identification and characterization of calreticulin as a novel plasminogen receptor. The Journal of Biological Chemistry, 300(1), 105465.

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