Log In Sign up
The largest database of trusted experimental protocols

The Delta-LacZ is a reporter gene that can be used to visualize gene expression patterns in Drosophila tissues. It encodes a truncated version of the beta-galactosidase enzyme, which catalyzes the hydrolysis of lactose into glucose and galactose. When the Delta-LacZ gene is expressed, the enzymatic activity of the truncated beta-galactosidase can be detected using histochemical staining techniques.

Automatically generated - may contain errors

Lab products found in correlation

Dl lacz, by Bloomington Drosophila Stock Center (2 mentions) Uas reaper, by Bloomington Drosophila Stock Center (1 mentions)

3 protocols using delta lacz

1

Fly Genetics: Lineage Tracing

Cited 1 time
Check if the same lab product or an alternative is used in the 5 most similar protocols
Fly stocks used in this study: w; Esg-Gal4, Tub-Gal80-ts, UAS-GFP ; UAS-Flp, Act>CD2>Gal4 (Esg FO) (Jiang et al., 2009 (link)), w; Esg-Gal4, Tub-Gal80-ts, UAS-GFP (Esgts) (Jiang and Edgar, 2009 (link)), Delta-LacZ (Dl-LacZ, Bloomington 11651), Gbe+Su(H)-lacZ (Su(H)-LacZ, Furriols and Bray, 2001 (link)). Flies were maintained at 25°C, on medium containing agar 0.6% (w/v), malt 6.5% (w/v), semolina 3.2% (w/v), baker’s yeast 1.8% (w/v), nipagin 2.4%, propionic acid 0.7%.
Viitanen A., Gullmets J., Morikka J., Katajisto P., Mattila J, & Hietakangas V. (2021). An image analysis method for regionally defined cellular phenotyping of the Drosophila midgut. Cell Reports Methods, 1(5), 100059.
+ Open protocol
+ Expand
2

Fly Genetic Toolkit for Stem Cell Research

Cited 2 times
Check if the same lab product or an alternative is used in the 5 most similar protocols
Fly stocks used in this study are as follows: w; esg-Gal4, Tub-Gal80ts, UAS-GFP ; UAS-Flp, Act>CD2>Gal4 (esgtsF/O) (25 (link)), w; esg-Gal4, Tub-Gal80ts, UAS-GFP (52 (link)), esg-Gal4, Tub-Gal80ts, Su(H)GBE-Gal80, 2xYFP (31 (link)), Delta-Gal4 (26 (link)), Delta-LacZ (Bloomington 11651), Gbe+Su(H)-lacZ [Su(H)-LacZ] (53 (link)), Raptor-RNAi (Bloomington 31529), Akt-RNAi (Bloomington 33615), Notch-RNAi (Bloomington 27988), UAS-GFP-E2F1; UAS-mRFP1-NLS-CycB (Fly-FUCCI) (29 (link)), UAS-Reaper (Bloomington 5824). Flies were maintained at 25°C, on medium containing agar 0.6% (w/v), malt 6.5% (w/v), semolina 3.2% (w/v), baker’s yeast 1.8% (w/v), nipagin 2.4%, and propionic acid 0.7%. In experiments using the temperature sensitive Gal80ts, flies were reared at +18°C and then kept at +29°C to inactivate the Gal80ts protein allowing the UAS-Gal4–driven transgene expression. The exact timings of the dietary treatment and temperature switch in each experiment are provided in the figure panels.
Mattila J., Viitanen A., Fabris G., Strutynska T., Korzelius J, & Hietakangas V. (2024). Stem cell mTOR signaling directs region-specific cell fate decisions during intestinal nutrient adaptation. Science Advances, 10(6), eadi2671.
+ Open protocol
+ Expand
3

Drosophila Intestinal Stem Cell Assay

Check if the same lab product or an alternative is used in the 5 most similar protocols
Fly stocks used in this study: w; Esg-Gal4, Tub-Gal80-ts, UAS-GFP ; UAS-Flp, Act>CD2>Gal4 (Esg FO) (Jiang et al., 2009) , w; Esg-Gal4, Tub-Gal80-ts, UAS-GFP (Esg ts ) (Jiang and Edgar, 2009) , Delta-LacZ (Dl-LacZ, Bloomington 11651), Gbe+Su(H)-lacZ (Su(H)-LacZ, Furriols and Bray, 2001) . Flies were maintained at 25°C, on medium containing agar 0.6% (w/v), malt 6.5% (w/v), semolina 3.2% (w/v), baker's yeast 1.8% (w/v), nipagin 2.4%, propionic acid 0.7%.
Viitanen A., Gullmets J., George J., Katajisto P., Mattila J., & Hietakangas V. (2021). LAM – an image analysis method for regionally defined organ-wide cellular phenotyping of the Drosophila midgut.
+ Open protocol
+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required

Sign up now

Revolutionizing how scientists
search and build protocols!