Fasting serum nonesterified fatty acids were transformed into fatty acid methyl esters as described previously [5 ,31 (link)]. Heptadecanoic acid (C17:0) was used as an internal standard. Fasting serum glucose, total cholesterol, triacylglycerol, high-density lipoprotein cholesterol, and low-density lipoprotein cholesterol levels were calculated with an automatic analyzer (Hitachi-7100, Hitachi, Tokyo, Japan). All kits were purchased from Biosino Biotechnology (Beijing, China). Serum insulin concentrations were measured using a mouse/rat insulin enzyme-linked immunosorbent assay kit (Cat. No. EZRMI-13K, Millipore, Burlington, MA, USA).
No ezrmi 13k
Manufactured by Merck Group
Sourced in United States
The EZRMI-13K is a laboratory instrument designed for routine analysis applications. It features a compact design and provides reliable performance for various laboratory tasks. The core function of this product is to assist researchers and scientists in their analytical workflows, but a detailed description without extrapolation or interpretation cannot be provided.
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Lab products found in correlation
2 protocols using no ezrmi 13k
1
Serum Fatty Acid and Lipid Analysis
2
Glucose Tolerance and Insulin Levels in Sod2 Mice
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Sod2+/++ mice were kindly provided by Dr. Robia Pautler (Baylor College of Medicine, Houston, TX, USA). They were bred in a C57BL6/J genetic background, carrying a human Sod2 transgene under the transcriptional control of the human β-actin promoter [19 (link)]. Mice were maintained under controlled environmental conditions (12:12 light:dark) with ad libitum access to food and drinking water. Experiments and procedures were conducted in accordance with European Directive 2012/63/UE and were approved by the Ethical Committee Board for Animal Experiments at the University of Oviedo (Experimental protocol PROAE 03/2016)
To measure glucose tolerance, 18-week-old mice fasted overnight (nmale = 5, nfemale = 3) received an intraperitoneal injection of glucose (2 mg/g body weight) and blood glucose was determined after 15, 30, 60, and 120 min from the tail vein using a One Touch Ultra EasyTM glucometer (Johnson&Johnson).
Insulin levels were measured in the plasma of overnight-fasted mice. Blood was extracted postmortem, collected in EDTA-treated tubes, and immediately centrifuged at 3000× g for 10 min at RT. Samples were frozen at −80 °C until analysis. Insulin levels were measured by ELISA following the manufacturer’s instructions (Millipore, catalogue no. EZRMI-13K).
To measure glucose tolerance, 18-week-old mice fasted overnight (nmale = 5, nfemale = 3) received an intraperitoneal injection of glucose (2 mg/g body weight) and blood glucose was determined after 15, 30, 60, and 120 min from the tail vein using a One Touch Ultra EasyTM glucometer (Johnson&Johnson).
Insulin levels were measured in the plasma of overnight-fasted mice. Blood was extracted postmortem, collected in EDTA-treated tubes, and immediately centrifuged at 3000× g for 10 min at RT. Samples were frozen at −80 °C until analysis. Insulin levels were measured by ELISA following the manufacturer’s instructions (Millipore, catalogue no. EZRMI-13K).
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