Fizz 1 antibody

The general reagents were purchased from Sigma-Aldrich (UK), unless stated otherwise. RPMI 1640, lipopolysaccharide (LPS), Gentamicin, MTT and NaNO₂, sodium nitroprusside (SNP), penicillin–streptomycin solution, and metformin were procured from Sigma-Aldrich. Recombinant mouse IFNγ cytokine is from eBiosciences (San Diego, CA, USA). CD11b+ human and Mouse MACS Microbeads and LC Columns are from Miltenyi Biotec. Primary antibodies including rabbit polyclonal NOS-2, rabbit polyclonal xIAP, cIAP-1, cIAP-2, LAMP-2, CD-206, rabbit polyclonal β-actin, and mouse monoclonal β-actin are from Santa Cruz biotechnology. Mouse monoclonal arginase-1 is from BD Biosciences. Rabbit monoclonal STAT1 and 3, pp38MAPK, and pNF-kB p65 are from Cell Signalling Technology. Ym-1 antibody was purchased from Stem cell technologies; Fizz-1 antibody was from Abcam. HRP-linked anti-mouse IgG and anti-rabbit IgG are from Cell Signalling Technology. TNFα and IL-10 ELISA kits were purchased from R&D system (Darmstadt, Germany).

RPMI 1640, Lipopolysaccharide (LPS), Penicillin Streptomycin solution, NaNO2, were purchased from Sigma (Taufkirchen, Germany). The Luminex kit was purchased from Bio-Rad Laboratories GmbH. Postfach 450133, D-80901 München, Germany, Sulphanilamide and N-(naphthyl) ethylene-diaminedihydrochloride were purchased from E-Merck (Darmstadt, Germany). Recombinant mouse TNF and IFN cytokines, Rabbit polyclonal anti-iNOs and anti-arginase-1 and anti-CD14 antibodies were purchased from BD transductions (Heidelberg, Germany). Rabbit anti-mouse pp65 (RelA), pp50 (nf-kb) antibodies were purchased from Cell signaling. Anti Actin antibody was purchased from Sigma and VEGFR2 antibody was purchased from Novus Biological. Rabbit anti-Ym-1 antibody was purchased from Stem cell technology; Fizz-1 antibody was purchased from Ab-cam. Anti-HIF-1 and 2 antibodies were purchased from Novus Biological Actin antibody was purchased from Sigma GmBH. Goat anti-rabbit HRP and Mouse HRP conjugated secondary antibodies were purchased from Sigma and Santa Cruz respectively.