Acetyl p53 lys379

Manufactured by Cell Signaling Technology

Sourced in Germany

Acetyl-p53 Lys379 is a lab equipment product that detects acetylation of p53 at lysine 379. It is used in research applications to study the regulation of p53 by post-translational modifications.

Automatically generated - may contain errors

Lab products found in correlation

Phospho p44 42 mapk erk1 2 thr202 tyr204, by Cell Signaling Technology (3 mentions) Histone h3, by Abcam (2 mentions) Stat3 79d7, by Cell Signaling Technology (2 mentions) Histone h3 acetyl k27, by Abcam (2 mentions) Supersignal west femto kit, by Thermo Fisher Scientific (2 mentions) P44 42 mapk erk1 2, by Cell Signaling Technology (2 mentions) Pvdf membrane, by Merck Group (2 mentions) β actin, by Abcam (2 mentions) Phospho stat3 tyr705, by Cell Signaling Technology (2 mentions) Horseradish peroxidase hrp, by GE Healthcare (2 mentions) Total p53, by Cell Signaling Technology (2 mentions) Gapdh, by Cell Signaling Technology (2 mentions) Sirt1, by Merck Group (2 mentions) Spike in antibody, by Active Motif (1 mentions) H4k16ac, by Abcam (1 mentions) Anti mouse igg hrp, by GE Healthcare (1 mentions) Ab1791, by Abcam (1 mentions) Ab1191, by Abcam (1 mentions) Ab4441, by Abcam (1 mentions) Ab18192, by Abcam (1 mentions)

Spelling variants of this product

Acetyl-p53 (18 citations) Anti-Acetyl-p53-Lys379 (3 citations)

7 protocols using acetyl p53 lys379

Western Blot Analysis of Cellular Proteins

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Whole cell lysates were prepared from 7 x 10⁵ cells in 2 x SDS-DTT sample buffer. Lysates were run on SDS-PAGE gels and transferred to PVDF membranes (Millipore) using standard protocols. Membranes were probed with the following primary antibodies at 1:1000 dilution unless otherwise stated: Acetyl-p53 Lys379 (1:500) (2570; Cell Signalling), total p53 (1C12; Cell Signalling), Phospho-p44/42 MAPK (Erk1/2) Thr202/Tyr204 (9101; Cell Signalling), p44/42 MAPK (Erk1/2) (9102; Cell Signalling), Phospho-Stat3 (Tyr705) (9131; Cell Signaling), Stat3 (79D7) (4904; Cell Signalling), Histone H3 (acetyl K27) (ab4729, Abcam), Histone H3 (ab1791, Abcam), Beta Actin (ab8227, Abcam). Membranes were probed with secondary antibodies conjugated to horseradish peroxidase (HRP) (GE Healthcare; 1:5000) and proteins detected using SuperSignal West Femto kit (Thermo Scientific).

Horton S.J., Giotopoulos G., Yun H., Vohra S., Sheppard O., Bashford-Rogers R., Rashid M., Clipson A., Chan W.I., Sasca D., Yiangou L., Osaki H., Basheer F., Gallipoli P., Burrows N., Erdem A., Sybirna A., Foerster S., Zhao W., Sustic T., Harrison A.P., Laurenti E., Okosun J., Hodson D., Wright P., Smith K.G., Maxwell P., Fitzgibbon J., Du M.Q., Adams D.J, & Huntly B.J. (2017). Early loss of Crebbp confers malignant stem cell properties on lymphoid progenitors. Nature cell biology, 19(9), 1093-1104.

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Chromatin Modification Antibody Validation

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H3K27ac (39,133, Active Motif Lot # 31814008), H3K27ac (39,685, Active Motif, no. 14517014), H3K18ac (ab1191, Abcam, no. GR3211480–1), H3K27me3 (9756, Cell Signaling), H3K9ac (ab4441, Abcam), H4K16ac (39,167, Active Motif), H3 general (ab1791, Abcam, no. GR177884–2), Spike-In antibody (61,686, Active Motif, Lot# 00419007), p300 (sc-584, Santa Cruz, Lot # F3016), Gapdh (2118, Cell Signaling, Lot # 10), CBP(D6C5) (7389S, Cell Signaling), p53(CM5) (NCL-L-p53-CM5p, Leica Biosystems), PKCs p2056 (ab18192, Abcam), KAP1/TRIM29 pS824 (A300-767A, Bethyl), Acetyl-p53 (Lys379) (2570, Cell Signaling), anti-mouse IgG-HRP (NA93V, GE, no.9773218), anti-rabbit IgG-HRP (170–6515, Bio-Rad, no. 350003248).

Martire S., Nguyen J., Sundaresan A, & Banaszynski L.A. (2020). Differential contribution of p300 and CBP to regulatory element acetylation in mESCs. BMC Molecular and Cell Biology, 21, 55.

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Murine Cytokine Signaling Pathway Assay

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Murine recombinant tumor necrosis factor-α (TNF-α) (#575204) and interleukin-1β (IL-1β) (#575102) were purchased from Biolegend. GSK126 was obtained from MedChemExpress. SB 203580 and PD98059 were purchased from AdooQ Bioscience.
The following primary antibodies were used : Lamin A/C (#2032), GAPDH (#2118), β-Actin (#4967), p65 (#6956), phospho-p65 (Ser536) (#3033), IKKβ (#2678), p53 (Rodent Specific) (#32532), phospho-p53 (Ser15) (#9284), IκBα (#4812), phospho-IκBα (#2859), NF-κB1 p105/p50 (#13586), NF-κB2 p100/p52 (#4882), phospho-p44/42 MAPK (Erk1/2) (Thr202/Tyr204) (#4370), phospho-p38 MAPK (Thr180/Tyr182) (#4511), phospho-SAPK/JNK (Thr183/Tyr185) (#4668), DYKDDDDK Tag (#2368), phospho-histone H2A.X (Ser139) (#9718), and acetyl-p53 (Lys379) (#2570), CDK6 (#3136), Ezh2 (#5246), RelB (#4922), all purchased from Cell Signaling Technology, and p21 (#sc-6246), which was purchased from Santa Cruz. Phospho-p53 (Ser20) (# PA5–104741), phospho-p53 (Ser37) (#HY-P80843) and phospho-RelA/p65 (Ser276) (#NB100–82086) antibodies were purchased from Invitrogen, MedChemExpress and Novus Biologicals, respectively.

Harada M., Su-Harada K., Kimura T., Ono K, & Ashida N. (2024). Sustained activation of NF-κB through constitutively active IKKβ leads to senescence bypass in murine dermal fibroblasts. Cell Cycle, 23(3), 308-327.

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Evaluating Protein Expression and Antioxidant Activity

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SLAB51 formulation was provided by Mendes S.A. (Lugano, Switzerland). PVDF membranes for Western blotting analyses were purchased from Millipore (Milano, Italy). Proteins immobilized on films were detected with the enhanced chemiluminescence (ECL) system (Amersham Pharmacia Biotech, Milano, Italy). SIRT1 (Merck Millipore, Darmstadt, Germany) and acetyl-p53(Lys379) antibodies were obtained from Cell Signaling Technology (Danvers, Massachusetts, USA). All other antibodies were from Santa Cruz Biotechnology (Heidelberg, Germany). The molecular probes’ protein molecular weight standards (6–205 kDa) (Sigma Aldrich, Italy) were used for molar mass calibration (it includes myosin 205 kDa, β-galactosidase 116 kDa, phosphorylase b 97 kDa, fructose-6-phosphate kinase 80 kDa, albumin 66 kDa, glutamic dehydrogenase 55 kDa, ovalbumin 45 kDa, carbonic anhydrase 30 kDa, trypsin inhibitor 21 kDa, lysozyme 14 kDa, aprotinin 6.5 kDa). Superoxide dismutase assay kit, reagents for antioxidant enzyme activities and protease inhibitors N-p-tosyl-phenylalanyl chloromethyl ketone (TPCK) and 4-(2-aminoethyl) benzenesulfonyl fluoride hydrochloride (AEBSF or Pefabloc) were from Sigma-Aldrich S.r.L. (Milano, Italy).

Bonfili L., Cecarini V., Cuccioloni M., Angeletti M., Berardi S., Scarpona S., Rossi G, & Eleuteri A.M. (2018). SLAB51 Probiotic Formulation Activates SIRT1 Pathway Promoting Antioxidant and Neuroprotective Effects in an AD Mouse Model. Molecular Neurobiology, 55(10), 7987-8000.

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Cardioprotective Mechanisms of Compound C

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H9c2 cells (rat cardiomyoblasts), obtained from ATCC (Manassas, VA), and were cultured in DMEM supplemented with 10% fetal bovine serum under an atmosphere of 95% air/5% CO₂ at 37°C. Compound C (Merck) was added to the medium for the times and at the doses indicated. The antibodies used included Sirt1, 8-OHdG goat polyclonal (Millipore), MnSOD (Upstate Biotechnology), Sirt1 rabbit polyclonal, Trx1, Troponin C mouse monoclonal (Santa Cruz), p53, Acetyl-p53 (Lys379), AMPKα and phospho-AMPKα(Thr172) (Cell Signaling), Nampt (Bethyl), Bcl-xL (Pharmingen), Bax (Abcam), FoxO1 rabbit monoclonal (Epitomics), and tubulin (Sigma).

Lu T.M., Tsai J.Y., Chen Y.C., Huang C.Y., Hsu H.L., Weng C.F., Shih C.C, & Hsu C.P. (2014). Downregulation of Sirt1 as aging change in advanced heart failure. Journal of Biomedical Science, 21(1), 57.

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Western Blot Analysis of Cellular Proteins

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Protein Expression Analysis Protocol

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Protein extraction and immunoblot analysis were performed using the standard protocol. Antibodies used were as follows: anti GAPDH (Millipore MAB374), Anti-ERG (Abcam ab92513), Anti-AR (Active Motif 39781), anti-PMEPA1 (ABNOVA H00056937-M01), Anti-PSA (DakoCytomation A05662), anti-p53 DO1 (Santa Cruz biotech, sc126), anti-SIRT1 and Acetyl-p53(Lys379) (Cell Signaling 2493 and 2570).

Kumar P., Sharad S., Petrovics G., Mohamed A., Dobi A., Sreenath T.L., Srivastava S, & Biswas R. (2016). Loss of miR-449a in ERG-associated prostate cancer promotes the invasive phenotype by inducing SIRT1. Oncotarget, 7(16), 22791-22806.

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