The quantitative analysis was performed by gas chromatography with flame ionization detector (Focus GC-FID, Thermo Scientific™). Sample solutions in hexane (2 μL/1000 μL) were prepared and 1.0 μL were injected under the following conditions: silica capillary column DB-5 MS (30 m × 0.25 mm × 0.25 μm), carrier gas: nitrogen (flow rate: 1 2 mL/min) injection mode split (20:1) temperature of column 60 to 240°C (range of 3°C/min) and injector and detector temperatures 250°C. For qualitative analysis of the essential oils gas chromatography-mass spectrometry (DSQ II GC-MS, Thermo Scientific™) was used. The analysis conditions for the injector and column were the same for GC-FID. The ionization source is electron impact (70 eV); transfer line temperature 200°C, helium carrier gas. The structural identification was made by comparison of their mass spectra and retention index to existing data in system libraries NIST 2011 and Adams 2007 [20 ,21 ].
Dsq 2 gc ms
Manufactured by Thermo Fisher Scientific
Sourced in United States
The DSQ II GC-MS is a gas chromatograph-mass spectrometer (GC-MS) system designed for analytical applications. It combines gas chromatography for sample separation and mass spectrometry for compound identification and quantification. The system enables the analysis of complex mixtures of volatile and semi-volatile organic compounds.
Automatically generated - may contain errors
Lab products found in correlation
Focus gc fid, by Thermo Fisher Scientific (1 mentions)
Q tof micro, by Waters Corporation (1 mentions)
Dc alufolien kieselgel 60 f254, by Merck Group (1 mentions)
Mercury plus 300, by Agilent Technologies (1 mentions)
400 mr ddr2, by Agilent Technologies (1 mentions)
Silicagel kieselgel 60, by Merck Group (1 mentions)
Ltq orbitrap velos, by Thermo Fisher Scientific (1 mentions)
5 protocols using dsq 2 gc ms
1
Quantitative and Qualitative Analysis of Essential Oils
2
GC/MS Metabolite Profiling Protocol
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Metabolite profiling was performed with a Thermo Scientific DSQ II GC/MS as exactly described in Lisec et al.69 (link) (please see also supporting information ). Metabolite extraction, analysis and identification were exactly as described69 (link).
3
Litsea cubeba Essential Oil Characterization
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Botrytis cinerea were obtained from China Microbial Culture Collection and maintained in slants of nutrient agar at 4 °C. Ethyl acetate, glutaric acid, osmium acid, and monometallic sodium orthophosphate were domestic analytical pure. Litsea cubeba fruits produced in Yongshun County, Hunan Province, China, and LCEO were extracted by using steam distillation. Gas chromatography-mass spectrometry (Thermo DSQII GC-MS, USA) was used to determine the LCEO components and content. These ingredients mainly include geranial (35.24%), neral (33.63%), limonene (9.05%), citronellal (3.14%), cineole (1.84%), linalool (1.80%), β-myrcene (1.69%), 1-caryophyllene (1.64%), α-pinene (1.22%), geraniol (1.10%), β-pinene (0.83%), nerol (0.81%), α-terpineol (0.61%), sabinene (0.48%), camphene (0.41%), caryophyllene oxide (0.29%), methyl-2-isopropenyl-4-hexenal (0.27%), β-elemene (0.27%), borneol (0.07%).
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Botrytis cinerea were obtained from China Microbial Culture Collection and maintained in slants of nutrient agar at 4 °C. Ethyl acetate, glutaric acid, osmium acid, and monometallic sodium orthophosphate were domestic analytical pure. Litsea cubeba fruits produced in Yongshun County, Hunan Province, China, and LCEO were extracted by using steam distillation. Gas chromatography-mass spectrometry (Thermo DSQII GC-MS, USA) was used to determine the LCEO components and content. These ingredients mainly include geranial (35.24%), neral (33.63%), limonene (9.05%), citronellal (3.14%), cineole (1.84%), linalool (1.80%), β-myrcene (1.69%), 1-caryophyllene (1.64%), α-pinene (1.22%), geraniol (1.10%), β-pinene (0.83%), nerol (0.81%), α-terpineol (0.61%), sabinene (0.48%), camphene (0.41%), caryophyllene oxide (0.29%), methyl-2-isopropenyl-4-hexenal (0.27%), β-elemene (0.27%), borneol (0.07%).
4
NMR and Mass Spectrometry Characterization
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NMR spectra were recorded on Varian Mercury
Plus 300 (299.97 MHz for 1H, 75.44 MHz for 13C, and 282.23 MHz for 19F) or Agilent 400-MR DDR2 (399.94
MHz for 1H, 100.58 MHz for 13C, and 376.29 MHz
for 19F) at 298 K unless otherwise indicated. Chemical
shifts δ are given in parts per million, using residual solvent
as an internal standard. 19F NMR and 31P NMR
chemical shifts were measured relative to CCl3F and H3PO4, respectively. Coupling constants J are reported in hertz. High-resolution mass spectra were obtained
on Q-Tof Micro (Waters), equipped with a quadrupole, TOF analyzers,
and an MCP detector or LTQ Orbitrap Velos (Thermo Fisher Scientific).
Gas chromatography–mass spectrometry (GC–MS) spectra
were obtained on GC–MS DSQ II (Thermo). Thin layer chromatography
analyses were carried out on DC Alufolien Kieselgel 60 F254 (Merck).
Preparative column chromatography separations were performed on silica
gel Kieselgel 60 of 0.040–0.063 mm (Merck). Melting points
were measured on a Boetius melting point apparatus and are uncorrected.
Starting materials, reagents, and substrates were obtained from commercial
suppliers and used without further purification. The solvents were
purified and dried using standard procedures. For synthesis of reagents,
see theSupporting Information .
Plus 300 (299.97 MHz for 1H, 75.44 MHz for 13C, and 282.23 MHz for 19F) or Agilent 400-MR DDR2 (399.94
MHz for 1H, 100.58 MHz for 13C, and 376.29 MHz
for 19F) at 298 K unless otherwise indicated. Chemical
shifts δ are given in parts per million, using residual solvent
as an internal standard. 19F NMR and 31P NMR
chemical shifts were measured relative to CCl3F and H3PO4, respectively. Coupling constants J are reported in hertz. High-resolution mass spectra were obtained
on Q-Tof Micro (Waters), equipped with a quadrupole, TOF analyzers,
and an MCP detector or LTQ Orbitrap Velos (Thermo Fisher Scientific).
Gas chromatography–mass spectrometry (GC–MS) spectra
were obtained on GC–MS DSQ II (Thermo). Thin layer chromatography
analyses were carried out on DC Alufolien Kieselgel 60 F254 (Merck).
Preparative column chromatography separations were performed on silica
gel Kieselgel 60 of 0.040–0.063 mm (Merck). Melting points
were measured on a Boetius melting point apparatus and are uncorrected.
Starting materials, reagents, and substrates were obtained from commercial
suppliers and used without further purification. The solvents were
purified and dried using standard procedures. For synthesis of reagents,
see the
5
Zearalenone Biodegradation Quantification
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Sample was prepared by modifying Mirocha et al. method [37 (link)]. Zearalenone (ZEA) working solution (32 µg mL−1) in acetonitrile was prepared for zearalenone biodegradation through GC-MS technique. The ZEA sample was treated with fresh bacterial cultures and incubated for 72 h at 37 °C. Working solution (500 µL) and 500 µL of nutrient broth having microbial culture were put in Eppendorf tubes and were kept in shaking incubator for 72 h at 37 °C. After 72 h, the sample was centrifuged at 12,000 rpm.
The culture supernatant containing ZEA (1.5 mL) was taken and acetonitrile+water was added in 86:14 v/v ratio. The mixture was kept for 24 h after capping. The extract (1.5 mL) was passed through a minicolumn syringe containing C-18:aluminium oxide in 1:3 w/w ratio. The elute (1 mL) was evaporated and dried after passing through dram tube for 1 h with nitrogen (Sigma-Aldrich, St. Louis, MO, USA). Then, 20 µL TMS reagent was added to the dried toxin. The vial was shaken, and the reaction was allowed to take place for 15 min. Isooctane 180 µL was added and then 200 µL sterilized distilled water was added to the reaction mixture. The mixture was vortexed to get a clear layer of isooctane. The transparent layer from above was transferred to a GC vial for analysis through GC-MS instrument “Thermo Scientific” (GC-MS) DSQ II Waltham, MA USA 02451.
The culture supernatant containing ZEA (1.5 mL) was taken and acetonitrile+water was added in 86:14 v/v ratio. The mixture was kept for 24 h after capping. The extract (1.5 mL) was passed through a minicolumn syringe containing C-18:aluminium oxide in 1:3 w/w ratio. The elute (1 mL) was evaporated and dried after passing through dram tube for 1 h with nitrogen (Sigma-Aldrich, St. Louis, MO, USA). Then, 20 µL TMS reagent was added to the dried toxin. The vial was shaken, and the reaction was allowed to take place for 15 min. Isooctane 180 µL was added and then 200 µL sterilized distilled water was added to the reaction mixture. The mixture was vortexed to get a clear layer of isooctane. The transparent layer from above was transferred to a GC vial for analysis through GC-MS instrument “Thermo Scientific” (GC-MS) DSQ II Waltham, MA USA 02451.
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