Bone marrow cells were prepared from the femurs of mice, and erythrocytes were depleted using ammonium chloride lysis buffer and then cocultured with CFSE‐labelled BaF3‐mock or BaF3‐RAE1ε cells at a ratio of 10:1 (bone marrow cells: BaF3 cells) in the presence of GM‐CSF (10 ng/ml) for 5 days. 10 μg/ml neutralizing anti‐NKG2D antibody (R&D Systems) was added into BaF3‐RAE1ε/bone marrow cells coculture system every 2 days as indicated. Resulting cells were stained and analysed using flow cytometry.
Neutralizing anti nkg2d antibody
Manufactured by R&D Systems
Sourced in United States
Neutralizing anti-NKG2D antibody is a laboratory reagent used to block the interaction between NKG2D receptor and its ligands. NKG2D is an activating receptor expressed on the surface of natural killer (NK) cells, CD8+ T cells, and other immune cells. This antibody neutralizes the NKG2D receptor, preventing its interaction with target cells expressing NKG2D ligands.
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Lab products found in correlation
2 protocols using neutralizing anti nkg2d antibody
1
Inhibiting NKG2D Modulates Bone Marrow Responses
2
BALB/c Mice Tumor Modeling with Anti-NKG2D
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BALB/c mice were injected subcutaneously with 4 × 106 BaF3‐mock or BaF3‐RAE1ε cells resuspended in PBS at day 0. In some groups, mice were injected with neutralizing anti‐NKG2D antibody (250 μg/mouse; R&D Systems, Minneapolis, MN, USA) or isotype‐matched control mAb (250 μg/mouse) through tail vein every 3 days. As indicated in some experiments, BALB/c mice were injected subcutaneously with 2 × 106 CT26 cells resuspended in PBS. At day 28 after injection, mice were killed for sorting MDSCs.
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