P53 antibody sc 126

Manufactured by Santa Cruz Biotechnology

Sourced in United States

The P53 antibody (sc-126) is a mouse monoclonal antibody that recognizes the p53 protein. The p53 protein is a tumor suppressor that plays a key role in regulating cell division and preventing the development of cancer. This antibody can be used to detect the p53 protein in various experimental applications.

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Lab products found in correlation

Propidium iodide, by Thermo Fisher Scientific (3 mentions) Rapamycin r0395, by Merck Group (2 mentions) β actin antibody a1978, by Merck Group (2 mentions) 03 gp62 c, by Santa Cruz Biotechnology (2 mentions) Sc 28359, by Santa Cruz Biotechnology (2 mentions) Hif2α antibody nb100 122, by Novus Biologicals (2 mentions) Atg7 antibody 8558, by Cell Signaling Technology (2 mentions) Beclin 1 antibody b6186, by Merck Group (2 mentions) Lamp1 antibody, by BD (2 mentions) Gfp antibody sc 8334, by Santa Cruz Biotechnology (2 mentions) Ha antibody prb 101p, by Fortrea (2 mentions) Bafilomycin a1 b 1080, by LC Laboratories (2 mentions) Hoechst 33342, by Thermo Fisher Scientific (2 mentions) Trypan blue solution, by Merck Group (1 mentions) Cep 1347, by Bio-Techne (1 mentions) Phospho pkcδ thr505, by Cell Signaling Technology (1 mentions) Marcks, by Cell Signaling Technology (1 mentions) Phospho pkc pan βii ser660, by Cell Signaling Technology (1 mentions) G 6976, by Merck Group (1 mentions) Phospho pkcα β 2 thr638 641, by Cell Signaling Technology (1 mentions)

5 protocols using p53 antibody sc 126

Antibody Sources and Reagents for Cell Signaling

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LC3B antibody was prepared by the Eissa laboratory as previously described (43 (link)). p62 antibody was from American Research Products, Inc (03-GP62-C) or Santa Cruz Biotechnology, Inc (sc-28359). HIF2α antibody (NB100-122) was from Novus Biologicals. VHL antibody (2738), S6 antibody (2117), P-S6 antibody (2211), beclin 1 antibody (3495), LDHA antibody (3558), Lamin A antibody (2032), ATG5 antibody (8540) and ATG7 antibody (8558) were from Cell Signaling Technology. ATG7 antibody (LS-B330) for immunohistochemistry was from LifeSpan Bioscences. Beclin 1 antibody (B6186) for immunohistochemistry, β-actin antibody (A1978) and rapamycin (R0395) were from Sigma. HA antibody (PRB-101P) was from Covance. LAMP1 antibody was form BD Biosciences Pharmingen (555798). GFP antibody (sc-8334) and p53 antibody (sc-126) were from Santa Cruz Biotechnology. Chromotek-GFP-Trap Resin Agarose (ACT-CM-GFA0050) was from Allele Biotechnology. Bafilomycin A1 (B-1080) was from LC Laboratories. MG132 (474790) was from Calbiochem.

Liu X.D., Yao J., Tripathi D.N., Ding Z., Xu Y., Sun M., Zhang J., Bai S., German P., Hoang A., Zhou L., Jonasch D., Zhang X., Conti C.J., Efstathiou E., Tannir N.M., Eissa N.T., Mills G.B., Walker C.L, & Jonasch E. (2014). Autophagy mediates HIF2α degradation and suppresses renal tumorigenesis. Oncogene, 34(19), 2450-2460.

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Antibody Sources and Reagents for Cell Signaling

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Molecular Signaling Pathway Inhibitors

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CEP-1347 was purchased from TOCRIS Bioscience (Bristol, UK). Gö6976, Crystal Violet (C.I. 42555), and Trypan Blue solution (0.4%) were obtained from Merck KGaA (Darmstadt, Germany). Propidium iodide and Hoechst33342 were supplied by Thermo Fisher Scientific (Waltham, MA, USA). An antibody against MDM4 (A700-000-T) was purchased from BETHYL (FORTIS LIFE SCIENCES, Waltham, MA, USA). An antibody against MDM2 (AF1244) was purchased from R&D Systems (Minneapolis, MN, USA). A p53 antibody (sc-126) was from Santa Cruz Biotechnology, Inc. (Santa Cruz, CA, USA). Antibodies against GAPDH (#5174), p21 Waf1/Cip1 (cyclin-dependent kinase inhibitor 1A, CDKN1A) (#2947), Myristoylated alanine-rich protein kinase C (PKC) substrate (MARCKS) (#5607), Phospho-MARCKS (Ser152/156) (#2741), Phospho-PKC (pan) (βII Ser660) (#9371), Phospho-PKCα/β II (Thr638/641) (#9375), Phospho-PKCδ (Thr505) (#9374), and Phospho-PKCθ (Thr538) (#9377) were from Cell Signaling Technology, Inc. (Beverly, MA, USA).

Togashi K., Suzuki S., Mitobe Y., Nakagawa-Saito Y., Sugai A., Takenouchi S., Sugimoto M., Kitanaka C, & Okada M. (2023). CEP-1347 Dually Targets MDM4 and PKC to Activate p53 and Inhibit the Growth of Uveal Melanoma Cells. Cancers, 16(1), 118.

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Co-immunoprecipitation of p53 with anti-iASPP

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The co-immunoprecipitation was performed using an ImmunoCruz^TM IP/WB Optima E System (Santa Cruz, UK), as previously described35 (link). 1 mg whole cell lysates were pre-cleared with irrelevant rabbit IgG (Life Technologies) for 2 hours and subsequently incubated overnight with anti-iASPP antibody (sc-53864; Santa Cruz), whereas rabbit IgG was used as the negative control. The bound proteins were analyzed by using Western blotting with p53 antibody (sc-126; Santa Cruz).

Dong P., Xiong Y., Watari H., Hanley S.J., Konno Y., Ihira K., Suzuki F., Yamada T., Kudo M., Yue J, & Sakuragi N. (2016). Suppression of iASPP-dependent aggressiveness in cervical cancer through reversal of methylation silencing of microRNA-124. Scientific Reports, 6, 35480.

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Evaluating Cancer Cell Signaling Pathways

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The cisplatin modified DNA (ab103261) and γH2A.X (ab26350) antibodies were from Abcam (Cambridge, USA). The phospho-STAT3 S727 (#9134), phospho-Akt S473 (#9271), phospho-NF-κB S536 (#3033), pERK T202/Y204 (#9101), pATF2 T71 (#5112), (P70S6K (#2708), phospho-P70S6K T389 (#9205), p21 (#2947), cleaved caspase 3 (#9661) and cleaved caspase 7 (#9491) antibodies were from Cell Signaling (MA, United States). The γH2A.X (S139) antibody (AB26350) was from Abcam (MA, USA). The p53 antibody (sc-126) was from Santa Cruz Biotechnology (TX, USA). The p63 antibody (NB100-691) was from Novus Biologicals (CO, USA). The actin monoclonal antibody (AC-15) was from Sigma-Aldrich (MO, United States). The SignalSilence p70/85 S6 Kinase siRNA was from Cell Signaling (MA, United States). The plasmids for FUCCI live cell imaging, mVenus-hGeminin(1/110) and mCherry-hCdt1(30/120), were a kind gift from Dr Atsushi Miyawaki (Riken, Japan). Dactolisib (NZP-BEZ235), MK2206, S3I-201, UO126 and SC75741 were all from Selleck Chem (MA, USA).

Hastings J.F., Gonzalez Rajal A., Latham S.L., Han J.Z., McCloy R.A., O'Donnell Y.E., Phimmachanh M., Murphy A.D., Nagrial A., Daneshvar D., Chin V., Watkins D.N., Burgess A, & Croucher D.R. (2020). Analysis of pulsed cisplatin signalling dynamics identifies effectors of resistance in lung adenocarcinoma. eLife, 9, e53367.

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