Acaricides used in this study were commercial formulations of bifenazate (Floramite, 240 g l−1 SC) and acequinocyl (Cantack 164 g l−1 SC), etoxazole (Borneo, 120 g l−1 SC), hexythiazox (Nissorun, 250 g l−1 SC) and clofentezine (Apollo, 500 g l−1 SC), abamectin (Vertimec 18 g l−1 EC), milbemectin (Milbeknock 10 g l−1 EC), bifenthrin (Talstar 100 g l−1 EC), fluvalinate (Mavrik 240 g l−1 EW) and analytical grade fenpropathrin (Sigma Aldrich).
Fenpropathrin
Manufactured by Merck Group
Sourced in United States
Fenpropathrin is a synthetic pyrethroid insecticide used for pest control. It is a clear to pale yellow liquid with a slight odor. Fenpropathrin is designed to be effective against a variety of insect pests.
Automatically generated - may contain errors
Lab products found in correlation
Deltamethrin, by Merck Group (3 mentions)
Bifenthrin, by Merck Group (3 mentions)
Cyhalothrin, by Merck Group (2 mentions)
Technical grade permethrin, by Merck Group (2 mentions)
Cypermethrin, by Merck Group (2 mentions)
Dimethoate, by Merck Group (2 mentions)
Imidacloprid, by Merck Group (2 mentions)
Chromatographic grade acetonitrile, by Thermo Fisher Scientific (1 mentions)
Fenvalerate, by Merck Group (1 mentions)
P nitrophenyl esters, by Merck Group (1 mentions)
E coli dh5α, by Thermo Fisher Scientific (1 mentions)
Pmd19 t, by Takara Bio (1 mentions)
β cypermethrin, by Merck Group (1 mentions)
Hplc grade acetonitrile, by Thermo Fisher Scientific (1 mentions)
Permethrin, by Merck Group (1 mentions)
Diazinon, by Merck Group (1 mentions)
Atrazine, by Merck Group (1 mentions)
Chlorpyrifos, by Merck Group (1 mentions)
Profenofos, by Merck Group (1 mentions)
Lindane, by Merck Group (1 mentions)
6 protocols using fenpropathrin
1
Acaricide Efficacy Evaluation Protocol
2
Pesticide Biodegradation Assay Protocol
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Technical-grade permethrin, deltamethrin, bifenthrin, fenpropathrin, cyhalothrin, and beta-cypermethrin were obtained from Sigma-Aldrich, USA. Chromatographic grade acetonitrile was purchased from Fisher Scientific, USA. All other reagents used here were obtained from Sigma-Aldrich, USA. The chemicals were dissolved in acetone or dimethyl sulfoxide (DMSO) at a stock concentration of 10 g·L−1, and stored in dark bottles at 4°C prior to use.
The mineral salt medium (MSM) containing 2 g (NH4)2SO4, 0.2 g MgSO4·7H2O, 0.01 g CaCl2·2H2O, 0.001 g FeSO4·7H2O, 1.5 g Na2HPO4·12H2O, and 1.5 g KH2PO4 per liter was used for biodegradation assays in the study. The Luria-Bertani medium (LB) containing 10 g tryptone, 5 g yeast extract and 10 g NaCl per liter was used for incubating Acinetobacter baumannii ZH-14. Both of the media were autoclaved at 121°C for 20 min. The pH of the media was adjusted to 7.0.
The mineral salt medium (MSM) containing 2 g (NH4)2SO4, 0.2 g MgSO4·7H2O, 0.01 g CaCl2·2H2O, 0.001 g FeSO4·7H2O, 1.5 g Na2HPO4·12H2O, and 1.5 g KH2PO4 per liter was used for biodegradation assays in the study. The Luria-Bertani medium (LB) containing 10 g tryptone, 5 g yeast extract and 10 g NaCl per liter was used for incubating Acinetobacter baumannii ZH-14. Both of the media were autoclaved at 121°C for 20 min. The pH of the media was adjusted to 7.0.
3
Isolation and Characterization of Carboxylesterases
Check if the same lab product or an alternative is used in the 5 most similar protocols
P. synxantha PS1 was isolated from oil well-produced water (Shengli Oilfield, Shandong Province, China). E. coli DH5α (Invitrogen, USA) and plasmid pMD19-T (TaKaRa, Japan) were used for gene cloning and sequencing of AT-containing and AT-truncated carboxylesterases. Plasmid pET-28a (Novagen, USA) was used as a vector to construct the protein expression plasmid in E. coli BL21 (DE3). The substrates fenpropathrin, cypermethrin, fenvalerate, bifenthrin, and p-nitrophenyl esters were purchased from Sigma (St. Louis, MO, USA). All other chemicals were of analytical grade and purchased from local markets.
+ Expand
P. synxantha PS1 was isolated from oil well-produced water (Shengli Oilfield, Shandong Province, China). E. coli DH5α (Invitrogen, USA) and plasmid pMD19-T (TaKaRa, Japan) were used for gene cloning and sequencing of AT-containing and AT-truncated carboxylesterases. Plasmid pET-28a (Novagen, USA) was used as a vector to construct the protein expression plasmid in E. coli BL21 (DE3). The substrates fenpropathrin, cypermethrin, fenvalerate, bifenthrin, and p-nitrophenyl esters were purchased from Sigma (St. Louis, MO, USA). All other chemicals were of analytical grade and purchased from local markets.
4
Biodegradation of Synthetic Pyrethroids
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
D-phenothrin (98% purity) was obtained from Jiangsu Yangnong Chemical Group Co., Ltd., China. Technical-grade permethrin, cyhalothrin, β-cypermethrin, deltamethrin, fenpropathrin, and bifenthrin were purchased from Sigma–Aldrich, United States and high-performance liquid chromatography (HPLC)-grade acetonitrile was purchased from Fisher Scientific, United States. All other chemicals and solvents were of analytical grade. SPs were dissolved in dimethyl sulfoxide (DMSO) or acetone at a stock concentration of 10 g⋅L-1, and stored in dark bottles at 4°C.
Mineral salt medium (MSM) containing (g⋅L-1) (NH4)2SO4, 2; MgSO4⋅7H2O, 0.2; CaCl2⋅2H2O, 0.01; FeSO4⋅7H2O, 0.001; Na2HPO4⋅12H2O, 1.5; and KH2PO4, 1.5; and Luria-Bertani (LB) medium containing (g⋅L-1) tryptone, 10; yeast extract, 5; and NaCl, 10 were used in this study. Both culture media were adjusted to pH 7.3 and sterilized at 121°C for 20 min.
Mineral salt medium (MSM) containing (g⋅L-1) (NH4)2SO4, 2; MgSO4⋅7H2O, 0.2; CaCl2⋅2H2O, 0.01; FeSO4⋅7H2O, 0.001; Na2HPO4⋅12H2O, 1.5; and KH2PO4, 1.5; and Luria-Bertani (LB) medium containing (g⋅L-1) tryptone, 10; yeast extract, 5; and NaCl, 10 were used in this study. Both culture media were adjusted to pH 7.3 and sterilized at 121°C for 20 min.
5
Pesticide Residue Analysis Protocol
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Pesticide reference standards including malathion, diazinon, profenofos, chlorpyrifos, chlorpyrifos-methyl, omethoate, dimethoate, cypermethrin, deltamethrin, fenpropathrin, permethrin, tetramethrin, cyfluthrin, fenvlarate, lamdacyhalothrin , lindane, heptachlor, aldrin, heptachlor epoxide, mixture endosulfan, dieldrin, endrin, o,P-DDT, carbaryl, carbofuran, bendiocarb, atrazine, imidacloprid, thiomethoxam and pendimethalin were purchased from Sigma Aldrich GmbH (Augsburg, Germany), Supelco and Bayer Crop Science , with certified purity ranging from 95% to 99%. Acetonitrile, acetone, sodium chloride, toluene, acetic acid and anhydrous magnesium sulfate were obtained from Scharlau (Barcelona, Spain). QuEChERS Finisterre micro centrifuge tube containing two or more of primary secondary amine (PSA), anhydrous magnesium sulfate (MgSO4) and graphed carbon black (GCB) with details of TR-Q2035 (150mg MgSO4, 25mg PSA, 2.5mg GCB) and TR-Q2015 (150mg MgSO4, 25mg PSA) were obtained from Teknokroma (Barcelona, Spain). Piperonyl Butoxide (PPB) with certified purity 95% used as internal standard was obtained from Sigma Aldrich GmbH.
All the organic solvents used were high performance liquid chromatography (HPLC) grade.
All the organic solvents used were high performance liquid chromatography (HPLC) grade.
6
Insecticide Preparation and Dilution Protocol
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Tested insecticides were analytical grade and included Bifenthrin (99.8%), Dimethoate (99.8%), fenpropathrin (99.1%), imidacloprid (99.9%), cyantraniliprole (98.0%), sulfoxaflor (99.5%), flupyradifurone (99.5%), and spinetoram (a mixture of 76.2% spinetoram J and 21.0% spintoram L) representing several insecticide classes (Table 1). Dimethoate, fenpropathrin, and imidacloprid were obtained from Sigma-Aldrich (St. Louis, Missouri). Bifenthrin, cyantraniliprole, sulfoxaflor, flupyradifurone, and spinetoram were obtained from Chem Service, Inc. (West Chester, Pennsylvania). Each insecticide was stored according to the manufacturer's recommendation. Serial dilutions of technical insecticide in acetone (ACS grade, 99.5%, Fisher Scientific, Hampton, New Hampshire) were made to generate test concentrations ranging from 0.0001 to 10,000 ng/µL. All insecticide solutions were kept at -20 °C for a maximum of 30 d and new solutions were prepared thereafter.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!