Dp71 digital ccd camera

Manufactured by Olympus

Sourced in Japan, United States

The DP71 is a digital CCD camera designed for microscopy applications. It features a high-resolution sensor and offers advanced image capture capabilities. The DP71 is capable of producing detailed, high-quality micrographs for various scientific and research purposes.

Automatically generated - may contain errors

Lab products found in correlation

Szx10 stereomicroscope, by Olympus (1 mentions) Spe confocal microscope, by Leica (1 mentions) Analysis five, by Olympus (1 mentions) Bx50 microscope, by Olympus (1 mentions) Micrometer, by Mitutoyo (1 mentions)

Close spelling variants of this product

DP71 CCD camera DP71 digital camera Digital CCD camera DP71 CCD DP71 digital DP71 camera CCD camera Digital camera

4 protocols using dp71 digital ccd camera

Visualizing β-galactosidase Activity in Whole Mounts

Cited 1 time

Check if the same lab product or an alternative is used in the 5 most similar protocols

Visualization of β-galactosidase activity with X-gal and endogenous GFP or YFP fluorescence in whole mounts has been described previously^{10 (link),33 (link)}. For X-gal staining, whole mount tissues were fixed for 30 min on ice with 4% PFA in PBS and washed with buffer A (2 mM MgCl₂ and 5 mM EDTA in PBS) twice for 5 min at room temperature. After incubation with buffer B (2 mM MgCl₂, 0.01% sodium deoxycholate and 0.02% Nonidet P40 in PBS) for 30 min, β-galactosidase activity was visualized with buffer C (5 mM potassium ferricyanide, 5 mM potassium ferrocyanide and 1 mg/ml X-gal in buffer B) at 37 °C overnight. Images of X-gal staining were acquired on an Olympus SZX10 stereomicroscope with a DP71 digital CCD camera. Fluorescence wholemount images were collected as z-stacks and projected into a single image using a Leica SPE confocal microscope.

Enomoto T., Nishida H., Iwata T., Fujita A., Nakayama K., Kashiwagi T., Hatanaka Y., Kondo H., Kajitani R., Itoh T., Ohmoto M., Matsumoto I, & Hirota J. (2019). Bcl11b controls odorant receptor class choice in mice. Communications Biology, 2, 296.

+ Open protocol

+ Expand

Quantifying Arterial Medial Calcification

Check if the same lab product or an alternative is used in the 5 most similar protocols

One piece (0.3–1 cm long) of the radial artery was collected during AVF surgery, and it was immediately fixed in 4% neutral formaldehyde. After fixation, the radial arteries were embedded in paraffin and cut with a rotating microtome at 2-μm thickness. The 2-μm-thick sections were stained with alizarin red S. Positive results indicated by an orange-red color were shown under the light microscope. An experienced pathologist evaluated the stained sections and examined them using an Olympus microscope (Olympus, Tokyo, Japan) in bright-field mode. The images were recorded using an Olympus DP-71 digital CCD camera, which was under software control, and medial calcification was graded by the pathologist on a semi-quantitative scale (0 = none; 1 = mild; 2 = moderate; and 3 = severe).

Chen Z., Zhou Y, & Yang T. (2021). Histopathological assessment of radial artery calcification in patients with end-stage kidney disease. Renal Failure, 43(1), 362-370.

+ Open protocol

+ Expand

TNCB-Induced Allergic Ear Inflammation Model

Check if the same lab product or an alternative is used in the 5 most similar protocols

Mice were sensitized by the application of 150 μL of 5% TNCB in an acetone-ethanol mixture (1:3 ratio) to the shaved abdomen (positive groups). Unsensitized mice fed with HFD or a normal diet (ND) for 8 weeks were used as negative controls. Four days later, all the mice were challenged on both ears with 10 μL of 0.4% TNCB in olive oil–acetone mixture (1:1 ratio). The ear thickness was measured prior to testing with a micrometer (Mitutoyo, Tokyo, Japan), by an observer unaware of the experimental groups and then again at 24 hours after challenge. Ear thickness was calculated as (Ear thickness [μm] 24 hours after challenge) — (Ear thickness [μm] before challenge). The ear swelling was expressed in μm ± standard error of the mean (SEM). Sections were examined under an Olympus BX50 microscope (Olympus, Japan). Images were recorded using a DP-71 digital CCD camera (Olympus, Japan) coupled to an IBM PC-class computer equipped with the AnalySIS-FIVE (Soft Imaging System GmbH, Münster, Germany) image analysis system.

Majewska-Szczepanik M., Kowalczyk P., Marcińska K., Strzępa A., Lis G.J., Susan Wong F., Szczepanik M, & Wen L. (2022). Obesity aggravates contact hypersensitivity reaction in mice. Contact dermatitis, 87(1), 28-39.

+ Open protocol

+ Expand

Quantitative Spinal Cord Inflammation Analysis

Check if the same lab product or an alternative is used in the 5 most similar protocols

The spinal cord sections were examined using Olympus BX50 brightfield/epifluorescence microscope (Olympus, Tokyo, Japan). All low magnification images were recorded with the use of Olympus DP71 digital CCD camera, stored as TIFF files and processed for quantitative analysis using ImageJ software (NIH, Bethesda, MD, USA).
In each image, inflammatory lesions characterized by high density of cells were marked (example in Figure 2a), and the percentage of immunopositive areas was assessed in the lesions. Only in the case of CD45, a general marker of inflammation, the percentage of immunopositive areas was estimated for the whole spinal cord cross-sectional area (Figure 2e).
A total of at least 20 sections were analyzed per experimental group (n = 5) and phase.

Pyka-Fościak G., Lis G.J, & Litwin J.A. (2022). Adhesion Molecule Profile and the Effect of Anti-VLA-4 mAb Treatment in Experimental Autoimmune Encephalomyelitis, a Mouse Model of Multiple Sclerosis. International Journal of Molecular Sciences, 23(9), 4637.

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!