Acridine orange

Cell culture media components such as DMEM, RPMI-1640 medium, Ham’s F12 K medium, penicillin, streptomycin, Hi-Sep Lymphocyte separation medium, HiKaryo XL RPMI medium, MTT, acridine orange, BSA, skimmed milk, prestained protein marker and tween 20 were purchased from HiMedia Laboratories, Mumbai, India. DMSO, trypan blue, Hoechst 33258, DCFH-DA, Rhodamine-123, Tris base, TRI reagent, Oligo(dT)-18 mer primers, Protease/phosphatase Inhibitor Cocktail and KBr were purchased from Sigma-Aldrich, USA. Fetal bovine serum, Dulbecco’s Phosphate Buffered Saline (DPBS), Trypsin–EDTA, Alexa Fluor 488 Annexin V/Dead Cell Apoptosis Kit, Fluo-3 AM, propidium iodide and Geltrex LDEV-Free Reduced Growth Factor Basement Membrane Matrix were purchased from Thermo Fisher Scientific, USA. M-MuLV reverse transcriptase enzyme, RNase A, dNTP mix and agarose were purchased from Genei Laboratories Pvt. Ltd, Bangalore, India. Triton X-100, EDTA, glycine, ethidium bromide, BCIP, NBT, silica gel for column chromatography (60–120 meshes), vanillin, sulphuric acid and solvents such as petroleum ether, chloroform, acetone, methanol (all HPLC grade) were procured from Sisco Research Laboratories Pvt. Ltd., Mumbai, India. Antibodies were procured from Cell Signaling Technology, Inc., USA and Sigma-Aldrich, USA. Primers sets were purchased from Eurofins Genomics India, Bangalore, India.

Malic acid, 1, 12-Dodecanedioic acid (DDA), Pluronic 127 were purchased from Sigma. Curcumin and Glycerol were purchased from TCI and Himedia, respectively. Primary (caspase-9 and GAPDH) and secondary antibodies were procured from Santa Cruz, US. Other chemicals used in animal cell culture studies, such as Dulbecco’s modified Eagle’s medium (DMEM), Fetal bovine serum (FBS), Trypsin-EDTA were purchased from Gibco, Thermo Fisher Scientific, India. Phosphate buffer saline, Acridine orange, Ethidium bromide and DAPI were obtained from Himedia, India. MCF-7 and MDA-MB-231 cell lines were procured from NCCS, Pune, India.

Farnesiferol-C was procured from Sigma Aldrich, USA. MTT dye (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide), Minimum Essential Media (MEM), iPurATM Total RNA Miniprep Purification Kit, RNase A, antibiotic–antimycotic solution, fetal bovine serum (FBS), acridine orange, and ethidium bromide were purchased from Himedia, India. Rhodamine (Rh)-123 and Hoechst 33342 were obtained from Sigma (St. Louis, MO, USA).