Neonatal human epidermal keratinocytes (HEKn; Thermo Fisher Scientific) were cultured in EpiLife medium (Thermo Fisher Scientific) supplemented with HuMedia-KG (Kurabo, Osaka, Japan) and grown to subconfluence. The culture medium was then replaced with EpiLife medium containing 6, 10, 20, or 40 mmol/l glucose, and the cells were cultured for an additional 3 days. After the cells were washed with PBS (-), samples were collected for further analyses.
Humedia kg
Manufactured by Kurabo
Sourced in Japan, United States
The HuMedia-KG is a laboratory equipment designed for the cultivation and maintenance of human cell lines. It provides a controlled environment for the growth and propagation of various human cell types. The HuMedia-KG offers precise temperature, humidity, and gas concentration regulation to support optimal cell culture conditions.
Automatically generated - may contain errors
Lab products found in correlation
Epilife medium, by Thermo Fisher Scientific (2 mentions)
Fetal bovine serum (fbs), by Kurabo (1 mentions)
Insulin, by Kurabo (1 mentions)
Gentamicin, by Kurabo (1 mentions)
Amphotericin b, by Kurabo (1 mentions)
Hydrocortisone, by Kurabo (1 mentions)
Mepi500ca, by Thermo Fisher Scientific (1 mentions)
Normal human epidermal keratinocytes, by Kurabo (1 mentions)
4 protocols using humedia kg
1
Glucose-Induced Keratinocyte Response
2
Normal Keratinocyte Culture and Treatment
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Normal human epidermal keratinocytes (NHKCs; Kurabo, Osaka, Japan) were cultured in HuMedia-KG supplemented with insulin (10 μg/mL), hEGF (0.1 ng/mL), hydrocortisone (0.5 μg/mL), gentamicin (50 μg/mL), amphotericin B (50 μg/mL), and fetal bovine serum (0.4% v/v; Kurabo). Cells were cultured at 37°C in a 5% CO2 atmosphere. Upon reaching 80% confluence, cells were treated with FICZ (10 nM), or DMBA (1 μM) for 4 h.
3
Glycogen Treatment of Keratinocytes
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Normal human epidermal keratinocyte cells were obtained from Kurabo Industries Ltd. (Osaka, Japan). Keratinocytes were cultured in EpiLifeTM medium with 60 μM calcium (MEPI500CA, Thermo Fisher Scientific Inc., Waltham, MA, USA) supplemented with or without growth factors of Humedia-KG (KK6150, Kurabo Industries Ltd.). The cells were seeded in 6-well dishes at a density of 1 × 105 cells/well and culture was carried out according to the manufacturer’s instructions, with exchange of the culture medium every 2 days. Sub-confluent cells were treated with or without 0.2, 1, or 2 % glycogen for 24 h or 7 days.
4
Paprika Oil Effects on Keratinocytes
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Normal human epidermal keratinocytes were obtained from Kurabo (Osaka, Japan) . Keratinocytes were cultured at 37℃ in EpiLife™ medium (MEPI500CA, Thermo Fisher Scientific, Waltham, MA, USA) supplemented with growth factors (Humedia-KG, Kurabo) . Commercially available red paprika xanthophyll oil was used (PapriX-oil) for the treatment of keratinocytes. The paprika oil was diluted to each concentration with dimethyl sulfoxide. At sub-confluence, cultured keratinocytes were stimulated with 0.05％, 0.005％, and 0.0005％ paprika oil. After 24 h, cells were collected and used for qRT-PCR and Western blot analyses.
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