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Rg217050

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RG217050 is a lab equipment product manufactured by OriGene. It is a device designed for laboratory use, but no further details about its core function can be provided in a concise, unbiased, and factual manner.

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Lab products found in correlation

Hct116, by OriGene (3 mentions) Pcmv6 ac gfp tagged cloning vector, by OriGene (3 mentions) Hct116, by American Type Culture Collection (2 mentions) Mccoy s 5a medium, by American Type Culture Collection (2 mentions) Atcc ccl 247, by American Type Culture Collection (1 mentions)

4 protocols using rg217050

1

Establishing DCLK1 Overexpression in HCT116 Cells

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Human colorectal carcinoma cell line HCT116 cells were purchased from ATCC and were maintained in McCoy’s 5A medium (ATCC® 30–2007™) supplemented with 10% FBS in 37 °C incubator with 5% CO2. Isogenic DCLK1 over-expressed cells were established by transfecting human DCLK1 variant 1 cDNA, which is fused with a turboGFP gene at C-terminal (OriGene, Cat # RG217050) into HCT116 cells. In order to avoid the clonal variance, different clones were selected. Control HCT116 cells were established by transfecting pCMV6-AC-GFP Tagged Cloning Vector (Origene, Cat # PS100010) into HCT116 cells. Both DCLK1 over-expressed cells and control HCT116 cells were selected (400ug/ml) and maintained (250ug/ml) using Geneticin.
Li L., Mei H, & Commey A.N. (2019). Application of RNA Sequencing to identify transcriptome modification by DCLK1 in Colorectal Cancer Cells. Cancer gene therapy, 27(9), 691-701.
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2

Establishment of DCLK1-overexpressing Colorectal Cancer Cells

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Human colorectal carcinoma cell line HCT116 cells were purchased from ATCC (ATCC® CCL-247™) and were maintained in McCoy’s 5A medium (ATCC® 30–2007™) supplemented with 10% FBS in 37°C incubator with 5% CO2. Isogenic DCLK1 over-expressed cells (DCLK1+) were established by transfecting human DCLK1 variant 1 cDNA, which is fused with a turboGFP gene at C-terminal (OriGene, Cat #RG217050) into HCT116 cells. In order to avoid the clonal variance, different DCLK1 over-expressed clones were selected. Control HCT116 cells (WT) were established by transfecting pCMV6-AC-GFP Tagged Cloning Vector (Origene, Cat #PS100010) into HCT116 cells. Both DCLK1 over-expressed cells and control HCT116 cells were selected (400 µg/ml) and maintained (250 µg/ml) using Geneticin (G418).
Li L., Jones K, & Mei H. (2019). Doublecotin-Like Kinase 1 Increases Chemoresistance of Colorectal Cancer Cells through the Anti-Apoptosis Pathway. Journal of stem cell research & therapy, 9(3), 447.
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3

DCLK1 Variant 1 Overexpression in Oral Cells

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Plasmid pCMV6-AC-GFP containing human DCLK1 variant 1 ORF (NM_004734) which is fused with a turboGFP at C-terminal (OriGene Technologies, Cat# RG217050) and control empty vector were transfected into OKF6 and NOK-SI cells with jetOPTIMUS DNA Transfection Reagent (Polyplus transfection, Cat# 76299-630).
Broner E.C., Trujillo J.A., Korzinkin M., Subbannayya T., Agrawal N., Ozerov I.V., Zhavoronkov A., Rooper L., Kotlov N., Shen L., Pearson A.T., Rosenberg A.J., Savage P.A., Mishra V., Chatterjee A., Sidransky D, & Izumchenko E. (2021). Doublecortin-Like Kinase 1 (DCLK1) Is a Novel NOTCH Pathway Signaling Regulator in Head and Neck Squamous Cell Carcinoma. Frontiers in Oncology, 11, 677051.
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4

Establishing DCLK1 Overexpression in HCT116 Cells

Check if the same lab product or an alternative is used in the 5 most similar protocols
Human colorectal carcinoma cell line HCT116 cells were purchased from ATCC and were maintained in McCoy’s 5A medium (ATCC® 30–2007™) supplemented with 10% FBS in 37 °C incubator with 5% CO2. Isogenic DCLK1 over-expressed cells were established by transfecting human DCLK1 variant 1 cDNA, which is fused with a turboGFP gene at C-terminal (OriGene, Cat # RG217050) into HCT116 cells. In order to avoid the clonal variance, different clones were selected. Control HCT116 cells were established by transfecting pCMV6-AC-GFP Tagged Cloning Vector (Origene, Cat # PS100010) into HCT116 cells. Both DCLK1 over-expressed cells and control HCT116 cells were selected (400ug/ml) and maintained (250ug/ml) using Geneticin.
Li L., Mei H, & Commey A.N. (2019). Application of RNA Sequencing to identify transcriptome modification by DCLK1 in Colorectal Cancer Cells. Cancer gene therapy, 27(9), 691-701.
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