Clkjrk

Manufactured by Bloomington Drosophila Stock Center

The ClkJrk is a laboratory equipment used for genetic research on circadian rhythms in Drosophila. It is a mutant allele of the Clock (Clk) gene, which plays a crucial role in regulating the internal biological clock of fruit flies. The ClkJrk mutation disrupts the normal function of the Clk gene, leading to alterations in the fly's circadian rhythm. This equipment is used by researchers to study the molecular mechanisms underlying the regulation of biological clocks in Drosophila.

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2 protocols using clkjrk

Drosophila Circadian Rhythm Mutants

Cited 2 times

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The following strains were used in this study: w¹¹¹⁸, Clk^Jrk, Pdf-Gal4, UAS-TrpA1, tubulin-Gal80^ts, Δ263b-Gal4/TM6B, Δ263a-Gal4/CyO, Bereft²⁴ [50 (link)], UAS-Bx and Bx loss-of-function mutation (Bx^hdpR26) [60 (link)]; UAS-mCD8::GFP, Bx-Gal4, Repo-Gal4, UAS-263b, and miR-263b^KO (obtained from the Bloomington Stock Center). All flies were raised on standard cornmeal/agar medium at 25 °C under 12-h:12-h LD cycle.

Nian X., Chen W., Bai W., Zhao Z, & Zhang Y. (2019). miR-263b Controls Circadian Behavior and the Structural Plasticity of Pacemaker Neurons by Regulating the LIM-Only Protein Beadex. Cells, 8(8), 923.

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Drosophila Rearing and Genetic Stocks

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Fly stocks were reared on standard cornmeal media (per 1L H₂0: 12g agar, 29g Red Star yeast, 71g cornmeal, 92g molasses, 16mL methyl paraben 10% in EtOH, 10mL propionic acid 50% in H₂0) at 25°C with 60% relative humidity and entrained to a daily 12hr light, 12hr dark schedule. Canton-S flies were from Gero Miesenböck (University of Oxford), w¹¹¹⁸ flies were from David Krantz (UCLA), LexAOP-CD8::GFP; UAS-flp, PBac{y[+mDint2]w[+mc]=brp(FRT.Stop)V5–2A-LexA-VP16} [52 (link)] was from Lawrence Zipursky (UCLA), yw,per⁰¹ [39 (link)] and yw; tim⁰¹ [38 (link)] were supplied by Amita Sehgal (University of Pennsylvania), and UAS-hiw^ΔRING [59 (link)] was from Aaron Diantonio (Washington University in St. Louis). UAS-hiw^RNAi [87 (link)] was ordered from the Vienna Drosophila Resource Center. w⁺, per⁰¹ [39 (link)], w⁺;tim⁰¹ [38 (link)], cyc⁰¹ [37 (link)], Clk^JRK [36 (link)], OR22a-GAL4 [20 (link)], UAS-kir2.1::GFP [44 (link)], tubP-GAL80^TS [46 (link)], UAS-CD8::GFP [88 (link),89 (link)], para^sbl−1 and para^sbl−2 [47 (link)], orco-GAL4 [45 (link)], hiw^ΔN [60 (link)], hiw^ND8 [59 (link)], and UAS-bsk^DN were obtained from the Bloomington Drosophila Stock Center.

Singh P, & Donlea J.M. (2020). Bidirectional regulation of sleep and synapse pruning after neural injury. Current biology : CB, 30(6), 1063-1076.e3.

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