Bacterial alkaline phosphatase type 3 e coli

PhIP-HCl was purchased from the Nard Institute (Osaka, Japan), and its purity was confirmed to be >99% by HPLC. AA was obtained from Sigma Aldrich (St. Louis, MO, United States). Nuclease P1 was purchased from Wako Pure Chemical Industries (Osaka, Japan). Phosphodiesterase I was purchased from Worthington. Nicotinamide, bovine spleen Phosphodiesterase II, DNase I, and bacterial alkaline phosphatase type III (E. coli) were purchased from Sigma Aldrich (St. Louis, MO, United States). Difco™ Nutrient Broth and Cofactor-I were acquired from Becton, Dickinson and Company (Sparks, MD, United States), and Oriental Yeast Co., Ltd (Tokyo, Japan), respectively. The S9 mix was purchased from the IEDA Trading Corporation (Tokyo, Japan). All other chemicals used in the study were of analytical grade and were purchased from Wako Pure Chemical Industries.

Bovine spleen phosphodiesterase II (SPD) and micrococcal nuclease (MN) were purchased from Worthington Biochemical Corp. (Lakewood, NJ, USA). Bacterial alkaline phosphatase Type III (E. coli), 2′-deoxycytidine, 2′-deoxythymidine, 2′-deoxyadenosine, 2′-deoxyGuanosine and 2′,3′-dideoxyinosine were purchased from Sigma-Aldrich Co. (St. Louis, MO, USA). Benzo[a]pyrene (>98%), adenosine (>98%), phosphate buffered saline(-) [PBS(-)], methanol (LC/MS grade), and dimethyl sulfoxide (DMSO) were purchased from Wako Chemical (Osaka, Japan). Guanosine (>98%), cytidine (>98%) and uridine (>98%) were purchased from Tokyo Chemical Industry Co (Tokyo, Japan).