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Zombie live dead kit

Manufactured by BioLegend
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The Zombie Live/Dead kit is a fluorescence-based cell viability assay that can distinguish between live and dead cells. It utilizes a membrane-impermeable dye to stain dead cells and a membrane-permeable dye to stain all cells. This allows for the quantification of live and dead cell populations.

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Lab products found in correlation

Live dead kit, by Thermo Fisher Scientific (2 mentions) Lsrii flow cytometer, by BD (1 mentions) Facsaria cell sorter, by BD (1 mentions)

Close spelling variants of this product

Live/Dead Zombie

2 protocols using zombie live dead kit

1

Flow Cytometry Staining Protocol

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For flow cytometry experiments (human and mouse), cells were pre-incubated with 2.4G2 mAb to block FcγR binding and then stained with panels of cell surface marker antibodies for 20 minutes on ice. Cells were washed 2x with FACS buffer and stained with LIVE/DEAD kit (Invitrogen) or Zombie LIVE/DEAD kit (BioLegend) to exclude dead cells. Intracellular staining was carried out using the FoxP3/Transcription factor Fix/Perm Kit (Tonbo). All samples were acquired with a LSRII flow cytometer (Becton Dickinson) and analyzed with FlowJo software version 9.6.2 or 10.6.1 (Tree Star).
Kansler E.R., Dadi S., Krishna C., Nixon B.G., Stamatiades E.G., Liu M., Kuo F., Zhang J., Zhang X., Capistrano K., Blum K.A., Weiss K., Kedl R.M., Cui G., Ikuta K., Chan T.A., Leslie C.S., Hakimi A.A, & Li M.O. (2022). Cytotoxic Innate Lymphoid Cells Sense Cancer Cell-Expressed Interleukin-15 to Suppress Human and Murine Malignancies. Nature immunology, 23(6), 904-915.
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2

FACS Sorting of Immune Cells

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Immune cells isolated from either human or mouse tissues were resuspended in MACS buffer (1x PBS with 1% FBS and 100 U/ml Penicillin G and 0.1 mg/ml Streptomycin) with appropriate cell surface marker antibodies and incubated for 20 minutes on ice. Cells were washed 2x in MACS buffer and stained with LIVE/DEAD kit (Invitrogen) or Zombie LIVE/DEAD kit (BioLegend) to exclude dead cells. Cells were sorted using the BD FACS Aria Cell Sorter. Samples were collected in RPMI with 10% FBS and processed immediately after sorting.
Kansler E.R., Dadi S., Krishna C., Nixon B.G., Stamatiades E.G., Liu M., Kuo F., Zhang J., Zhang X., Capistrano K., Blum K.A., Weiss K., Kedl R.M., Cui G., Ikuta K., Chan T.A., Leslie C.S., Hakimi A.A, & Li M.O. (2022). Cytotoxic Innate Lymphoid Cells Sense Cancer Cell-Expressed Interleukin-15 to Suppress Human and Murine Malignancies. Nature immunology, 23(6), 904-915.
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