Carboxymethyl β cyclodextrin

DOPE (1,2-dioleoyl-sn-glycero-3-phospho ethanol amine and DOTAP (1,2-dioleoyl-3-trimethylammonium-propane) were purchased from Lipoid (Germany). Carboxymethyl-β-cyclodextrin, Pluronic-F12, cholesterol, DMEM, Fetal bovine serum (FCS), L- Glutamine and Opti-MEM I Reduced-Serum Medium were purchased from Sigma Aldrich (UK). pc DNA3.1-GFP and maxi prep kit were purchased from thermofisher scientific, UK, COS7 and SH-SY5Y cell lines were obtained from ATCC, UK. E coli, ampicillin containing agar plated, Luria-Bertaini and agarose were also provided by University of Sunderland. TransIT-LT1 liposomal transfection reagents were purchased from Mirus Bio LLC Madison, (USA), Promega QuantiFluor® ONE dsDNA System was purchased from Promega (UK).

Standard vinblastine sulfate (VB) working standard (99.65% purity) was kindly obtained from the National Organization of Drug Development and Research. Analytical grade reagents and solvents were employed in the study. Poly(vinyl)chloride (PVC), phosphomolybdic acid (PM), phosphotungstic acid (PT), sodium tetraphenylborate (TPB), ammonium reineckate (RK), potassium tetrakis (TKS), nitrophenyl octyl ether (NPOE), dioctyl phthalate (DOP), dibutyl phthalate (DBP), dibutyl sebacate (DBS), nitrophenyl phenyl ether (NPPE), hydroxypropyl-β-cyclodextrin (HPBCD), β-cyclodextrin (BCD), carboxymethyl-β-cyclodextrin (CMBCD), calix-[8]-arene (CX8) and tetrahydrofuran (THF) were obtained from Sigma-Aldrich. Sodium hydroxide, potassium dihydrogen phosphate, and hydrochloric acid were obtained from (El-Nasr Pharmaceutical Chemical Company, Cairo, Egypt). A 50 mM KH₂PO₄ solution adjusted to pH 4.0 ± 0.1 was used as a buffer. Plasma samples were purchased from Vacsera Co. (Giza, Egypt).