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Tauroursodeoxycholic acid

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Tauroursodeoxycholic acid is a bile acid that is commonly used in laboratory research. It is a white to off-white crystalline powder with a molecular formula of C₂₆H₄₅NO₆S and a molecular weight of 515.71 g/mol. Tauroursodeoxycholic acid is soluble in water, methanol, and ethanol.

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Lab products found in correlation

Glycoursodeoxycholic acid, by Steraloids (3 mentions) Taurocholic acid, by Steraloids (3 mentions) Glycodeoxycholic acid, by Steraloids (3 mentions) Taurodeoxycholic acid, by Steraloids (3 mentions) Taurohyocholic acid, by Steraloids (2 mentions) Hyocholic acid, by Steraloids (2 mentions) Taurohyoxydeoxycholic acid, by Steraloids (2 mentions) Ursodeoxycholic acid, by Steraloids (2 mentions) Hyodeoxycholic acid, by Steraloids (2 mentions) Chenodeoxycholic acid, by Steraloids (2 mentions) Optima grade acetonitrile, by Thermo Fisher Scientific (2 mentions) Tauro beta muricholic acid, by Steraloids (2 mentions) Tauro omega muricholic acid, by Steraloids (2 mentions) Alpha muricholic acid, by Steraloids (2 mentions) Beta muricholic acid, by Steraloids (2 mentions) Ammonium acetate, by Merck Group (2 mentions) Deoxycholic acid, by Steraloids (1 mentions) Deoxycholic acid, by Merck Group (1 mentions) Ursodeoxycholic acid, by Merck Group (1 mentions) Hyodeoxycholic acid, by Merck Group (1 mentions)

3 protocols using tauroursodeoxycholic acid

1

Quantitative Bile Acid Analysis

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Optima grade acetonitrile and water were purchased from Fisher Scientific (Loughborough, UK). Ammonium acetate was purchased from Sigma-Aldrich (Dorset, UK). Formic acid was purchased from Fluka (Buchs, Switzerland). Bile acid standards, including tauro-alpha-muricholic aicd (T-α-MCA), tauro-beta-muricholic acid (T-β-MCA), tauro-omega-muricholic acid (T-ω-MCA), alpha-muricholic acid (α-MCA), beta-muricholic acid (β-MCA), taurohyocholic acid (THCA), glycoursodeoxycholic acid (GUDCA), tauroursodeoxycholic acid (TUDCA), hyocholic acid (HCA), taurohyoxydeoxycholic acid (THDCA), taurocholic acid (TCA), cholic acid (CA), ursodeoxycholic acid (UDCA), hyodeoxycholic acid (HDCA), glycodeoxycholic acid (GDCA), taurodeoxycholic acid (TDCA), chenodeoxycholic acid (CDCA), and deoxycholic acid (DCA) were purchased from Steraloids, Inc. (Newport, RI). Standard solutions of these 20 bile acids were prepared at concentrations of 0.5, 10, 50, 100, 500, 1500, 5000, and 10 000 nM in a solution of 20% (v/v) acetonitrile and water.
Poland J.C., Schrimpe-Rutledge A.C., Sherrod S.D., Flynn C.R, & McLean J.A. (2019). Utilizing Untargeted Ion Mobility-Mass Spectrometry To Profile Changes in the Gut Metabolome Following Biliary Diversion Surgery. Analytical chemistry, 91(22), 14417-14423.
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2

Bile Acid Profiling with LC-MS

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Optima grade acetonitrile and water were purchased from Fisher Scientific (Loughborough, UK). Ammonium acetate was purchased from Sigma-Aldrich (Dorset, UK). Formic acid was purchased from Fluka (Buchs, Switzerland). Bile acid standards were purchased from Steraloids, Inc. (Newport, RI) and included tauro-alpha-muricholic acid (T-α-MCA), tauro-beta-muricholic acid (T-β-MCA), tauro-omega-muricholic acid (T-ω-MCA), alpha-muricholic acid (α-MCA), beta-muricholic acid (β-MCA), taurohyocholic acid (THCA), glycoursodeoxycholic acid (GUDCA), tauroursodeoxycholic acid (TUDCA), hyocholic acid (HCA), taurohyoxydeoxycholic acid (THDCA), taurocholic acid (TCA), cholic acid (CA), ursodeoxycholic acid (UDCA), hyodeoxycholic acid (HDCA), glycodeoxycholic acid (GDCA), taurodeoxycholic acid (TDCA), chenodeoxycholic acid (CDCA), and deoxycholic acid (DCA).
Poland J.C., Leaptrot K.L., Sherrod S.D., Flynn C.R, & McLean J.A. (2020). Collision Cross Section Conformational Analyses of Bile Acids via Ion Mobility–Mass Spectrometry. Journal of the American Society for Mass Spectrometry.
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3

Quantifying Bile Acid Profiles

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BA standards including cholic acid (CA), chenodeoxycholic acid (CDCA), deoxycholic acid (DCA), lithocholic acid (LCA), ursodeoxycholic acid (UDCA) and hyodeoxycholic acid (HDCA) were purchased from Sigma-Aldrich Chemicals Co (Steinheim, Germany). α-muricholic acid (α-MCA), β-muricholic acid (β-MCA), ω-muricholic acid (ω-MCA), tauro-cholic acid (T-CA), tauro-chenodeoxycholic acid (T-CDCA), tauro-deoxycholic acid (T-DCA), tauro-lithocholic acid (T-LCA), tauro-ursodeoxycholic acid (T-UDCA), tauro-α-muricholic acid (T-α-MCA), tauro- β-muricholic acid (T-β-MCA), glyco-cholic acid (G-CA), glyco-chenodeoxycholic acid (G-CDCA), glyco-deoxycholic acid (G-DCA), glyco-lithocholic acid (G-LCA), glyco-ursodeoxycholic acid (G-UDCA) and deuterated internal standards (IS) lithocholic acid-2,2,4,4-D4 (LCA-D4), deoxycholic acid-2,2,4,4-D4 (DCA-D4) and glycochenodeoxycholic acid-2,2,4,4-D4 (GCDCA-D4) were obtained from Steraloids, Inc. (Newport, RI, USA). Methanol HPLC grade was from Merck (Darmstadt, Germany). Ultra-pure reagent water was purified by a Milli-Q gradient system (Millipore, Bedford, MA, USA). Acetonitrile LC-MS grade was from VWR and formic acid LC-MS grade was purchased from Sigma Aldrich Chemicals Co.
Ghaffarzadegan T., Essén S., Verbrugghe P., Marungruang N., Hållenius F.F., Nyman M, & Sandahl M. (2019). Determination of free and conjugated bile acids in serum of Apoe(−/−) mice fed different lingonberry fractions by UHPLC-MS. Scientific Reports, 9, 3800.
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