Hla a2 fitc

Manufactured by BioLegend

Sourced in United Kingdom

HLA-A2-FITC is a fluorescently-labeled antibody that binds to the HLA-A2 allele, a common human leukocyte antigen. It is used to identify and quantify cells expressing HLA-A2 by flow cytometry.

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FITC anti-human HLA-A2 (4 citations) FITC anti-human HLA-A2 antibody (4 citations) FITC-conjugated anti-HLA-A2 (3 citations) Anti-HLA-A2–FITC (clone BB7.2; (3 citations) Anti-HLA-A2 FITC antibody (2 citations)

2 protocols using hla a2 fitc

Multicolor Flow Cytometry Immunophenotyping

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The following antihuman antibodies were used for cell staining: CD3-PECy7 or CD3-AF700, CD4-FITC, CD8-APC, CD45-PE, CD56-PE, iNKT TCR (Vα24-Jα18 TCR)-APC, CD45RA-FITC, CD62L-PECy7, TIM3-FITC, PD1-APC, LAG3-PECy7, HLA-A2-FITC, HLA-A3-APC, HLA-B7-PECy7, and Annexin V-FITC or Annexin V-Pacific Blue and were purchased from BioLegend. Data acquisition was performed using either a BD Accuri C6 Flow cytometer (BD Biosciences) or an Attune NXT cytometer (Thermo Fisher). Flow cytometry data were analyzed using FlowJo software (Tree Star).

Fang K.K., Lee J., Khatri I., Na Y, & Zhang L. (2023). Targeting T-cell malignancies using allogeneic double-negative CD4-CAR-T cells. Journal for Immunotherapy of Cancer, 11(9), e007277.

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Differential Immunophenotyping of NK Cell Subsets

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The following antibodies were used. From eBioscience (San Diego, CA): CD3-APC eFluor 780 (clone SK7); CD16-FITC (eBioCB16); CD19-APC eFluor 780 (HIB19); CD45-PE (HI30); CD94-FITC (DX22); Eomes-PE eFluor 610 (WD1928); Granzyme K-PerCP eFluor 710 (G3H69); HLA-A3-FITC (GAP.A3); IFN gamma-Alexa Fluor 488 (4S.B3); S1PR1-eFluor 660 (SW4GYPP); T-bet-PECy7 (4B10); TNF alpha-APC (Mab11). From Biolegend (London, UK): CCR5-APC (J418F1); CD49a-FITC (TS2/7); CD69-APC (FN50); CD103-FITC (Ber-ACT8); CX3CR1-FITC (2A9-1); CXCR6-PerCP Cy5.5 (K041E5); CXCR6-APC (K041E5); GM-CSF-PE (BDV-21C11); Granzyme B-FITC (GB11); HLA-A2-FITC (BB7.2); KIR2DL1/S1/S3/S5-APC (HP-MA4); KIR2DL2/L3-APC (DX27); KIR3DL1-APC (DX9); Perforin-APC (dG9). From BD (Oxford, UK): CD56-BV510 (NCAM16.2), LIF-PE (1F10). Dead cells were excluded using Fixable Viability Dye eFluor 450 (eBioscience). Intracellular staining was carried out using Human FoxP3 Buffer (BD) according to the manufacturer’s instructions. Data were acquired on a Fortessa II (BD) and analysed using FlowJo (Treestar, Ashland, OR). Cells were sorted on an Aria (BD). Eomes^lo NK cells were isolated by sorting on live cells (propidium iodide negative, Tonbo Biosciences, San Diego, CA), singlets, scatter, CD3^- CD56⁺ CXCR6^- CD16⁺. Eomes^hi NK cells were isolated by sorting on live cells, singlets, scatter, CD3^- CD56⁺ CXCR6⁺.

Cuff A.O., Robertson F.P., Stegmann K.A., Pallett L.J., Maini M.K., Davidson B.R, & Male V. (2016). Eomeshi NK Cells in Human Liver Are Long-Lived and Do Not Recirculate but Can Be Replenished from the Circulation. The Journal of Immunology Author Choice, 197(11), 4283-4291.

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