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Nis elements d package 3

Manufactured by Nikon
Sourced in Japan

NIS-Elements D Package 3.00 is Nikon's comprehensive software solution for image acquisition, processing, and analysis. It provides a versatile platform for managing and utilizing digital imaging data from a variety of Nikon microscopes and cameras.

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2 protocols using nis elements d package 3

1

Fungal Species Identification Protocol

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Species identification was based on morphological characters of the conidiomata and ascomata produced on infected plant tissues. Cross-sections were prepared by hand using a double-edge blade under a Leica stereomicroscope (M205 FA; Wetzlar, Germany). Photomicrographs were captured with a Nikon Eclipse 80i microscope equipped with a Nikon digital sight DS-Ri2 high definition color camera, using differential interference contrast (DIC) illumination and the Nikon software NIS-Elements D Package 3.00 (Tokyo, Japan). Measurements of ascospores and conidia are reported as maximum and minimum in parentheses and the range representing the mean ± standard deviation of the number of measurements given in parentheses. Cultural characteristics of isolates incubated on PDA in the dark at 25 C were recorded.
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2

Morphological Identification of Fungal Species

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Species identification was based on the morphological characters of apothecia and conidiomata produced on natural substrates. Cross-sections were prepared manually using a double-edged blade under a Leica stereomicroscope (M205 FA). Photomicrographs were captured with a Nikon Eclipse 80i microscope equipped with a Nikon digital sight DS-Ri2 high-definition colour camera, using differential interference contrast (DIC) illumination and the Nikon software, NIS-Elements D Package 3.00. Measurements of ascospores and conidia are reported as the maximum and minimum in parentheses and the range representing the mean ± standard deviation of the number of measurements is given in parentheses. Cultural characteristics of isolates incubated on MEA in the dark at 25 °C were recorded.
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