Hek293t cells

Human embryonic kidney 293 T (HEK293T) and Jurkat T cells were purchased from the American Type Culture Collection (Manassas, VA, USA). HEK293T cells were cultured in a 10% CO₂ incubator at 37 °C in Dulbecco’s modified Eagle’s medium (DMEM, Welgene, Gyeongsan, Korea). The culture medium contained 10% fetal bovine serum (FBS, Welgene) and 1% penicillin/streptomycin (P/S, Hyclone). Jurkat T cells were cultured in a 5% CO₂ incubator at 37 °C, in RPMI1640 medium (Gibco, Thermo Fisher Scientific) supplemented with 10% FBS and 1% P/S.

The human lung cancer A549 cells, colorectal cancer DLD-1 cells, colon cancer RKO cells, embryonic kidney HEK293T cells, normal skin Detroit-551 cells, and murine Lewis lung carcinoma LLC cells were purchased from the American Type Culture Collection (Manassas, VA, USA). The DLD-1 cells, RKO cells, Detroit-551 cells, LLC cells, and HEK293T cells were maintained in Dulbecco’s modified Eagle’s medium (Welgene, Gyeongsan, Korea). The A549 cells were cultured in RPMI 1640 medium (HyClone™; GE Healthcare Life Sciences, Logan, UT, USA). All culture media were supplemented with 10% heat-inactivated fetal bovine serum (Welgene) and antibiotics (100 U/mL penicillin and 100 μg/mL streptomycin; Thermo Fisher Scientific, Rockford, IL, USA). The cells were maintained in a humidified 5% CO₂ incubator at 37 °C.