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Spexin

Manufactured by Phoenix Pharmaceuticals
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Sourced in United States

Spexin is a laboratory instrument used for the detection and quantification of specific molecules or compounds within a sample. It utilizes advanced spectroscopic techniques to provide accurate and reliable analytical data.

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Lab products found in correlation

Fatty acid free bovine serum albumin, by Roche (2 mentions) Collagenase type 1, by Merck Group (2 mentions) Secondary antibodies for western blot, by Merck Group (1 mentions) Anti β actin a2228, by Merck Group (1 mentions) Anti erk1 2 sc 514302, by Santa Cruz Biotechnology (1 mentions) Deoxycholate, by Merck Group (1 mentions) Lithocholate, by Merck Group (1 mentions) Taurodeoxycholate, by Merck Group (1 mentions) Taurolithocholate, by Merck Group (1 mentions) Cholate, by Merck Group (1 mentions) Taurocholate, by Merck Group (1 mentions) Glycochenodeoxycholic acid, by Merck Group (1 mentions) Glycocholate, by Merck Group (1 mentions) Tauroursodeoxycholate, by Merck Group (1 mentions) Chenodeoxycholate, by Merck Group (1 mentions) Taurochenodeoxycholate, by Merck Group (1 mentions) Ursodeoxycholate, by Merck Group (1 mentions) Glycodeoxycholate, by Merck Group (1 mentions)

4 protocols using spexin

1

Galanin Receptor Antagonists in Myogenesis

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Media and supplements were from Corning (Tewksbury, MA, USA). Spexin was purchased from Phoenix Pharmaceuticals. Galanin receptor antagonists were purchased from Tocris Bio-techne. Antibodies: anti-myogenin (sc-12732), anti-Myh (sc-376157) and anti-MyoD (sc-377460), anti-vinculin (sc-73614), anti-GALR2 (AF1463a), anti-GALR3 (AP16443), anti-phospho-ERK1/2 (sc-7383) and anti- ERK1/2 (sc-514302) were from Santa Cruz Biotechnology (Dallas, TX, USA) or Abgent (San Diego, CA, USA); anti-βactin A2228 and all secondary antibodies for Western blot were from Sigma-Aldrich (Taufkirchen, Germany). Unless otherwise stated all other reagents were from Sigma-Aldrich.
Leciejewska N., Pruszyńska-Oszmałek E., Mielnik K., Głowacki M., Lehmann T.P., Sassek M., Gawęda B., Szczepankiewicz D., Nowak K.W, & Kołodziejski P.A. (2021). Spexin Promotes the Proliferation and Differentiation of C2C12 Cells In Vitro—The Effect of Exercise on SPX and SPX Receptor Expression in Skeletal Muscle In Vivo. Genes, 13(1), 81.
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2

Radiolabeled Oleic Acid and Spexin Protocols

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9,10-[3H]-Oleic acid (OA) was purchased from NEN Life Science Products (Boston, MA, USA), type I collagenase from Sigma (St. Louis, MO, USA), and fatty acid free bovine serum albumin (BSA) from Boehringer Mannheim (Indianapolis, IN, USA).
Two preparations of Spexin were used: the first from Phoenix Pharmaceuticals (Burlingame, CA) and the second, a custom synthesis product from the Ferring Research Institute (San Diego, CA). Both were >99% pure by HPLC and ID'd by LC/MS.
Walewski J.L., Ge F., Lobdell H I.V., Levin N., Schwartz G.J., Vasselli J., Pomp A., Dakin G, & Berk P.D. (2014). Spexin is a Novel Human Peptide that Reduces Adipocyte Uptake of Long Chain Fatty Acids and Causes Weight Loss in Rodents with Diet-induced Obesity. Obesity (Silver Spring, Md.), 22(7), 1643-1652.
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3

Radiolabeled Oleic Acid and Spexin Protocols

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9,10-[3H]-Oleic acid (OA) was purchased from NEN Life Science Products (Boston, MA, USA), type I collagenase from Sigma (St. Louis, MO, USA), and fatty acid free bovine serum albumin (BSA) from Boehringer Mannheim (Indianapolis, IN, USA).
Two preparations of Spexin were used: the first from Phoenix Pharmaceuticals (Burlingame, CA) and the second, a custom synthesis product from the Ferring Research Institute (San Diego, CA). Both were >99% pure by HPLC and ID'd by LC/MS.
Walewski J.L., Ge F., Lobdell H I.V., Levin N., Schwartz G.J., Vasselli J., Pomp A., Dakin G, & Berk P.D. (2014). Spexin is a Novel Human Peptide that Reduces Adipocyte Uptake of Long Chain Fatty Acids and Causes Weight Loss in Rodents with Diet-induced Obesity. Obesity (Silver Spring, Md.), 22(7), 1643-1652.
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4

Bile Acid Standards for Mass Spectrometry

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A series of bile acid standards, containing cholate (CA), chenodeoxycholate (CDCA), ursodeoxycholate (UDCA), deoxycholate (DCA), lithocholate (LCA), glycocholate (GCA), glycochenodeoxycholic acid (GCDCA), glycodeoxycholate (GDCA), taurocholate (TCA), taurodeoxycholate (TDCA), tauroursodeoxycholate (TUDCA), taurohyodexoycholate (THDCA), taurochenodeoxycholate (TCDCA), taurolithocholate (TLCA) were purchased from Sigma-Aldrich (St. Louis, MO, USA). Tauro-β-muricholate (TβMCA) was purchased from Santa Cruz Biotechnology (Santa Cruz, CA, USA). As internal standard (IS), deoxycholic acid-2,2,4,4-d4 (DCA-d4) were obtained from CDN isotopes (Pointe-Claire, Quebec, Canada). All organic reagents for mass spectrometric analysis were HPLC grade purchased from Sigma-Aldrich (St. Louis, MO, USA). And spexin used for drug treatment was purchased from Phoenix Pharmaceuticals (Belmont, CA, USA). M871 was purchased from R&D Systems (Minneapolis, MN, USA). SNAP37889 was purchased from Key Organics Ltd (Camelford, Cornwall, UK).
Lin C.Y., Zhao L., Huang T., Lu L., Khan M., Liu J., Zhong L.L., Cai Z.W., Fan B.M., Wong A.O, & Bian Z.X. (2018). Spexin Acts as Novel Regulator for Bile Acid Synthesis. Frontiers in Physiology, 9, 378.
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