In the in vivo T cell subsets depletion study, InVivoMAb anti-mouse CD4 (YTS191, BE0119, BioXCell), CD8α (YTS169.4, BE0117 BioXcell) antibodies were intraperitoneally injected at a dose of 200 μg per mouse. To neutralize Cxcl10 in vivo, 50 µg of anti-mouse Cxcl10 (134013, MAB466, R&D systems) was intraperitoneally injected into mice every 3 days for 2 weeks.
Mab466
Manufactured by R&D Systems
MAB466 is a monoclonal antibody that recognizes human CD25. CD25 is the alpha subunit of the interleukin-2 receptor complex, which is expressed on activated T cells and regulatory T cells. This antibody is intended for research use only.
Automatically generated - may contain errors
Lab products found in correlation
Mab006, by R&D Systems (2 mentions)
Invivomab, by BioXCell (1 mentions)
Be0119, by BioXCell (1 mentions)
Be0117, by BioXCell (1 mentions)
Mab478, by R&D Systems (1 mentions)
Af 492 na, by R&D Systems (1 mentions)
1 step ultra tmb, by Thermo Fisher Scientific (1 mentions)
Streptavidin hrp, by BD (1 mentions)
Maxisorp plate, by Thermo Fisher Scientific (1 mentions)
Bovine serum albumin (bsa), by Thermo Fisher Scientific (1 mentions)
Multiskan ms, by Thermo Fisher Scientific (1 mentions)
Duoset elisa kit, by R&D Systems (1 mentions)
5 protocols using mab466
1
In Vivo T Cell Depletion and Cxcl10 Neutralization
2
In Vivo Immunomodulation Strategies
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To neutralize CCL5, CXCL9, and CXCL10 in vivo, anti-mouse CCL5 (R&D Systems, MAB478, 50 μg per mouse), anti-mouse CXCL9 (R&D Systems, AF-492-NA, 50 μg per mouse), anti-mouse CXCL10 (R&D Systems, MAB466, 50 μg per mouse) or a mixture of these antibodies were intraperitoneally injected twice weekly.
For CD40/CD40L signal blockade in vivo, an anti-mouse CD40L monoclonal antibody (BioXCell, MR-1, 300 μg per mouse) was intraperitoneally administered twice weekly.
To deplete CD4+ T cells or CD8+ T cells, mice were injected intraperitoneally with 400 μg of an anti-mouse CD4 antibody (BioXCell, GK1.5) or an anti-mouse CD8 antibody (BioXCell, YST-169.4) every 3 days.
For CD40/CD40L signal blockade in vivo, an anti-mouse CD40L monoclonal antibody (BioXCell, MR-1, 300 μg per mouse) was intraperitoneally administered twice weekly.
To deplete CD4+ T cells or CD8+ T cells, mice were injected intraperitoneally with 400 μg of an anti-mouse CD4 antibody (BioXCell, GK1.5) or an anti-mouse CD8 antibody (BioXCell, YST-169.4) every 3 days.
3
CXCL10 Neutralization Inhibits Concanavalin A
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4
CXCL10 Deficiency Impacts NASH
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CXCL10-/- and C57BL/6 wildtype (WT) mice were randomly fed with MCD diet to induce NASH or the corresponding control diet supplemented with choline bitartrate (2g/kg) and DL-methionine (3g/kg) (ICN Biomedicals, Costa Mesa, CA) for 4 weeks. CXCL10-/- and WT mice were also fed with high-fat high-cholesterol diet (HFHC) (Specialty feeds, Glen Forrest, WA, Australia) or normal chow for 8 weeks. In another experiment, WT mice were administered with anti-CXCL10 monoclonal antibodies (mAb) (MAB466, R&D systems, Minneapolis, MN) or control mAb (MAB006, R&D systems) by intraperitoneal injection (50 μg per mouse) for 10 days after induction of steatohepatitis by feeding mice with the MCD diet for 3 weeks. Animals were housed in a temperature-controlled room under a 12h light /12h dark cycle and had free access to food and water. All animal studies were performed in accordance with guidelines approved by the Animal Experimentation Ethics Committee of the Chinese University of Hong Kong.
5
ELISA-based Detection of Chemokines
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Example 9
For detection of mCXCL10(1-77), Maxisorp Plates® (Nunc) were coated with 4 μg/ml of α-mCXCL10 capturing antibody (MAB466, R&D) in PBS and incubated over night at 4° C. Plates were washed twice with 300□l of PBS. Blocking was done with BSA 1% (proteinase free, Gibco), in PBS for 2 h at room temperature. Plates were washed 3 times with 300 μl of 0.05% Tween-20 in PBS. Tumor and plasma samples were diluted in BSA 1% and incubated for 2 h, at room temperature. To obtain a standard curve, and to control for the cross-reactivity of the detection antibody, dilutions of recombinant mCXCL10 (Peprotech) or DPP4-digested mCXCL10(3-77) were incubated in parallel. For detection of mCXCL10(1-77), biotinylated α-mCXCL10(1-77) (AbDSerotec, 0.5 μg/ml, clone AbD17185.1), Streptavidin-HRP (BD Biosciences) and 1-Step Ultra TMB (Thermo Scientific) were used. Enzymatic reactions were stopped with HCL 1N and plates were read with 450 nm in a Lab-systems Multiskan MS (Thermo) reader. For detection of total mCXCL10, the mCXCL10 DuoSet® ELISA kit (R&D) or a combination of capturing α-mCXCL10 (MAB466) and biotinylated α-mCXCL10 (BAF466, both from R&D) were used, unless otherwise indicated. Detection of mDPP4, mCCL22 and mCXCL12 was done with the DuoSet® ELISA kit (R&D). Detection of mVEGF, mCCL2 and mCCL3 was done with a multiplex kit (Invitrogen).
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