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Dmem f12 powder

Manufactured by Thermo Fisher Scientific
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DMEM/F12 powder is a cell culture medium formulation used to support the growth and maintenance of a variety of cell types in vitro. It provides essential nutrients, salts, and other components required for cell proliferation and survival.

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Lab products found in correlation

Zidovudine, by Merck Group (1 mentions) Bleomycin, by Cayman Chemical (1 mentions) Chloramphenicol, by Merck Group (1 mentions) Aztreonam, by Cayman Chemical (1 mentions) Dna from salmon testes, by Merck Group (1 mentions) Acridine orange, by Merck Group (1 mentions) Hepes buffer, by Merck Group (1 mentions) Sybr safe dna stain, by Thermo Fisher Scientific (1 mentions) Micrococcal nuclease, by Merck Group (1 mentions) Dnase 1, by Merck Group (1 mentions) Etoac, by Merck Group (1 mentions) Trypan blue staining kit, by Beyotime (1 mentions) Inverted phase contrast microscope, by Olympus (1 mentions) Pkh26, by Merck Group (1 mentions) Effectene, by Qiagen (1 mentions) Facscalibur flow cytometer, by BD (1 mentions)

3 protocols using dmem f12 powder

1

DNA Manipulation and Cell Culture Protocols

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Acridine orange, chloramphenicol, 4′,6-diamidino-2-phenylindole (DAPI), DNA from salmon testes, HEPES buffer, LB EZ Mix agar, and zidovudine were from Sigma-Aldrich (now Millipore Sigma). DNase I, from the bovine pancreas, and micrococcal nuclease, were also both from Sigma-Aldrich. Aztreonam and bleomycin were from Cayman Chem, Ann Arbor, MI. Agarose for DNA gels was SeaKem LE agarose from Lonza Co., Rockland, ME. Antibiotic MIC Strips were from Liofilchem, Waltham, MA.
DMEM/F12 powder was from the Gibco Division of Thermo-Fisher, Grand Island, NY, USA. The composition of this medium is available at https://www.thermofisher.com/us/en/home/technical-resources/media-formulation.329.html, accessed on 16 March 2023. The DMEM/F-12 was supplemented with 18 mM NaHCO3 and 25 mM of additional HEPES, pH 7.4. Sybr Safe DNA stain, 100 bp DNA ladders, and 10 X loading dye (“Blue Juice”) were from the Invitrogen Division of Thermo-Fisher.
Crane J.K, & Catanzaro M.N. (2023). Role of Extracellular DNA in Bacterial Response to SOS-Inducing Drugs. Antibiotics, 12(4), 649.
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2

Evaluation of Anti-Inflammatory Effects

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Dichloromethane (DCM with p.a grade and purity of purchased from Merck), methanol (MeOH p.a, Merck), Ethyl acetate (EtOAc p.a, Merck), n-butanol (BuOH p.a, Merck), dimethyl sulfoxide p.a, (Merck), Dulbecco’s modified Eagle’s medium (DMEM/F12) powder Gibco Life Technologies, 10% Fetal Bovine Serum (FBS), 1% penisilin-streptomicin, Lipopolysaccharide LPS E. coli O111:B4 (List Biological Laboratory, Inc.), Griess Reagent Kit for Nitrite Determination G-7921 (Thermo Fisher Scientific).
Tanod W.A., Yanuhar U., M.a.f.t.u.c.h., Putra M.Y, & Risjani Y. (2019). Screening of NO Inhibitor Release Activity from Soft Coral Extracts Origin Palu Bay, Central Sulawesi, Indonesia. Anti-Inflammatory & Anti-Allergy Agents in Medicinal Chemistry, 18(2), 126-141.
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3

Isolation and Characterization of Umbilical Cord-Derived Cells

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Umbilical cords were collected from full-term, healthy neonates during caesarean sections. Informed consent was obtained before enrolment in the study. The following equipment was used: an inverted phase contrast microscope (Olympus, Tokyo, Japan), atomic force microscope (Thermomicroscopes, Sunnyvale, CA, USA), FACSCalibur flow cytometer (BDBiosciences, San Jose, CA, USA) and PhiliPCM-10 transmission electron microscope (Philips, Amsterdam, Holland). The following reagents were used: DMEM/F12 powder (GIBCO BRL, Gaithersburg, MD, USA), alizarin red and oil red O staining solutions (Guangzhou Sijia Biotech Co., Ltd., Guangzhou, China), Gd-DTPA injection (Bayer, Leverkusen, North Rhine-Westphalia, Germany), Effectene (Qiagen, Hilden, North Rhine-Westphalia, Germany), trypan blue staining kit (Beyotime Institute of Biotechnology), PKH26 (Sigma, St. Louis, MO, USA), and 1.5T HDXT MR (GE Healthcare, Milwaukee, WI, USA).
Shuai H., Shi C., Lan J., Chen D, & Luo X. (2015). Double labelling of human umbilical cord mesenchymal stem cells with Gd-DTPA and PKH26 and the influence on biological characteristics of hUCMSCs. International journal of experimental pathology, 96(1).
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